<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2022 Workiva-->
<!--r:285fa4de-18b4-4cbb-be23-ead68a6c10b3,g:f89a3579-bc58-41d2-a53f-1ab5ec74300b-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://asana.com/role/Cover" xlink:type="simple" xlink:href="asan-20220131.xsd#Cover"/>
  <link:definitionLink xlink:role="http://asana.com/role/Cover" xlink:type="extended" id="ifb1fc68fb69941d1a3958e2553c3fb2b_Cover">
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_a29d2e14-1dbd-4268-8373-7bf5f717046c" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_DocumentType_a29d2e14-1dbd-4268-8373-7bf5f717046c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentAnnualReport_2b226191-106d-4608-b2f4-b5bc7125b625" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentAnnualReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_DocumentAnnualReport_2b226191-106d-4608-b2f4-b5bc7125b625" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_6078c828-742e-4a97-ab9d-db22edeb7ea8" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_DocumentTransitionReport_6078c828-742e-4a97-ab9d-db22edeb7ea8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_f6b78943-ce58-4f17-be60-40e1ad6b1dd9" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_DocumentPeriodEndDate_f6b78943-ce58-4f17-be60-40e1ad6b1dd9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_4fdccf66-3bad-4ee4-aebb-c86aefb824e6" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_CurrentFiscalYearEndDate_4fdccf66-3bad-4ee4-aebb-c86aefb824e6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_b02e4e09-99f5-499a-b45e-221e283d1d51" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityFileNumber_b02e4e09-99f5-499a-b45e-221e283d1d51" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_beb196d7-ad07-47c9-b3fe-ee410adb9089" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityRegistrantName_beb196d7-ad07-47c9-b3fe-ee410adb9089" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_8a17542b-5c99-4459-a069-26eee774aec5" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityIncorporationStateCountryCode_8a17542b-5c99-4459-a069-26eee774aec5" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_cc09ea29-92a4-465f-b73b-0cc13b88193e" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityTaxIdentificationNumber_cc09ea29-92a4-465f-b73b-0cc13b88193e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_53c2b22e-9408-41df-a84c-97e39667bf11" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityAddressAddressLine1_53c2b22e-9408-41df-a84c-97e39667bf11" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_ae3248f5-9992-4607-8ccd-91100d43e9a7" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityAddressCityOrTown_ae3248f5-9992-4607-8ccd-91100d43e9a7" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_16943605-eaed-49a7-ad41-1a78c1efcb16" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityAddressStateOrProvince_16943605-eaed-49a7-ad41-1a78c1efcb16" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_0e6d2414-19b0-4cf7-9c8a-e1fab530003b" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityAddressPostalZipCode_0e6d2414-19b0-4cf7-9c8a-e1fab530003b" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_bf6fac47-f5b2-4714-b95f-a462a3130326" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_CityAreaCode_bf6fac47-f5b2-4714-b95f-a462a3130326" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_cbe715be-996f-42ee-ba8a-789e89401c7c" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_LocalPhoneNumber_cbe715be-996f-42ee-ba8a-789e89401c7c" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_8fc593db-416a-4815-8249-7200f84ffc86" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_Security12bTitle_8fc593db-416a-4815-8249-7200f84ffc86" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_96613277-ddc5-4bb4-b9ff-8d8b7f88f2fc" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_TradingSymbol_96613277-ddc5-4bb4-b9ff-8d8b7f88f2fc" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_ba530053-c5fc-41e4-9dc1-8ac12434e227" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_SecurityExchangeName_ba530053-c5fc-41e4-9dc1-8ac12434e227" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityWellKnownSeasonedIssuer_5c4d7b7b-c99c-4091-91f3-5a6c4730155b" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityWellKnownSeasonedIssuer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityWellKnownSeasonedIssuer_5c4d7b7b-c99c-4091-91f3-5a6c4730155b" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityVoluntaryFilers_b61ecc69-ff83-4673-a29e-8ab3d8934d7b" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityVoluntaryFilers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityVoluntaryFilers_b61ecc69-ff83-4673-a29e-8ab3d8934d7b" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_9c89b8ec-12f0-4de6-806d-3a58d7503456" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityCurrentReportingStatus_9c89b8ec-12f0-4de6-806d-3a58d7503456" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_f9654198-44c1-4f48-82a7-d8b62aa868d8" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityInteractiveDataCurrent_f9654198-44c1-4f48-82a7-d8b62aa868d8" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_06f6208f-0c86-4f38-8b26-91af1e1fa8d5" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityFilerCategory_06f6208f-0c86-4f38-8b26-91af1e1fa8d5" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_3a0ff209-8f62-4ff0-8f83-903e83c5dab3" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntitySmallBusiness_3a0ff209-8f62-4ff0-8f83-903e83c5dab3" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_b907870f-7c26-49b4-b68f-ac6938819d92" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityEmergingGrowthCompany_b907870f-7c26-49b4-b68f-ac6938819d92" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_IcfrAuditorAttestationFlag_0e36364c-8ea8-498e-9604-009d73463b2b" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_IcfrAuditorAttestationFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_IcfrAuditorAttestationFlag_0e36364c-8ea8-498e-9604-009d73463b2b" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_5619f57c-847d-41c7-9b30-cf101f36491d" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityShellCompany_5619f57c-847d-41c7-9b30-cf101f36491d" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityPublicFloat_b7f1335c-b4f4-46e9-b783-e51c29108a87" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityPublicFloat"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityPublicFloat_b7f1335c-b4f4-46e9-b783-e51c29108a87" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_b31ff9d3-1bec-4997-adaa-cdeefcc07c83" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_b31ff9d3-1bec-4997-adaa-cdeefcc07c83" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentsIncorporatedByReferenceTextBlock_7e8dde37-4c23-43be-bbbe-35ebc2bb78f8" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentsIncorporatedByReferenceTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_DocumentsIncorporatedByReferenceTextBlock_7e8dde37-4c23-43be-bbbe-35ebc2bb78f8" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_273a9a88-3728-485e-92d2-d7bf62a3bd3c" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_EntityCentralIndexKey_273a9a88-3728-485e-92d2-d7bf62a3bd3c" xlink:type="arc" order="30"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_e949fe5c-8566-4249-9c94-f11135e054a1" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_DocumentFiscalYearFocus_e949fe5c-8566-4249-9c94-f11135e054a1" xlink:type="arc" order="31"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_b996e9d2-1bb5-4724-8b8a-2729d64f4017" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_DocumentFiscalPeriodFocus_b996e9d2-1bb5-4724-8b8a-2729d64f4017" xlink:type="arc" order="32"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_99fc84ce-05bc-40bb-b551-2b3f021fa8ac" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_AmendmentFlag_99fc84ce-05bc-40bb-b551-2b3f021fa8ac" xlink:type="arc" order="33"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationTable_5a217fda-f3b9-4c7d-9d76-45cd6f8defb7" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentInformationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_DocumentInformationLineItems_8845e386-fb40-48da-9acc-41cbc123d88f" xlink:to="loc_dei_DocumentInformationTable_5a217fda-f3b9-4c7d-9d76-45cd6f8defb7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_8c5a5ee9-bcb9-4d59-80c4-ab7ff672e4da" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_DocumentInformationTable_5a217fda-f3b9-4c7d-9d76-45cd6f8defb7" xlink:to="loc_us-gaap_StatementClassOfStockAxis_8c5a5ee9-bcb9-4d59-80c4-ab7ff672e4da" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_8c5a5ee9-bcb9-4d59-80c4-ab7ff672e4da_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_8c5a5ee9-bcb9-4d59-80c4-ab7ff672e4da" xlink:to="loc_us-gaap_ClassOfStockDomain_8c5a5ee9-bcb9-4d59-80c4-ab7ff672e4da_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_c8f61d95-e270-4063-9ac6-8f52a73f6443" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_8c5a5ee9-bcb9-4d59-80c4-ab7ff672e4da" xlink:to="loc_us-gaap_ClassOfStockDomain_c8f61d95-e270-4063-9ac6-8f52a73f6443" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_f0586cc7-05cb-4d3d-83d2-3d2379a7e238" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_c8f61d95-e270-4063-9ac6-8f52a73f6443" xlink:to="loc_us-gaap_CommonClassAMember_f0586cc7-05cb-4d3d-83d2-3d2379a7e238" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_b6936a3c-39eb-4b67-a30c-71315e38bf02" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_c8f61d95-e270-4063-9ac6-8f52a73f6443" xlink:to="loc_us-gaap_CommonClassBMember_b6936a3c-39eb-4b67-a30c-71315e38bf02" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/ConsolidatedStatementsofRedeemableConvertiblePreferredStockandStockholdersEquityDeficit" xlink:type="simple" xlink:href="asan-20220131.xsd#ConsolidatedStatementsofRedeemableConvertiblePreferredStockandStockholdersEquityDeficit"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConsolidatedStatementsofRedeemableConvertiblePreferredStockandStockholdersEquityDeficit" xlink:type="extended" id="if7387c25188b4761a1263e0e1f96b007_ConsolidatedStatementsofRedeemableConvertiblePreferredStockandStockholdersEquityDeficit">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_d9465dbc-baf5-4a15-a853-77461f9de73f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_d9465dbc-baf5-4a15-a853-77461f9de73f" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_0686f12b-be4e-46d5-aa10-c216f474ba05" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_0686f12b-be4e-46d5-aa10-c216f474ba05" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_28e8771f-f982-4283-a2e6-6e616c741a8c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_StockholdersEquity_28e8771f-f982-4283-a2e6-6e616c741a8c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_a39d40b0-ff88-4d0b-8144-88a75e8f73c3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_a39d40b0-ff88-4d0b-8144-88a75e8f73c3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_c1479651-47f0-4308-9a37-2c874c76344c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_c1479651-47f0-4308-9a37-2c874c76344c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions_9c57c8ae-38ba-4fa2-bbd9-4e78800f716f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions_9c57c8ae-38ba-4fa2-bbd9-4e78800f716f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodShares_6ca7a042-14ee-45e5-8e5d-d9ea4f2af7f9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockRepurchasedDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodShares_6ca7a042-14ee-45e5-8e5d-d9ea4f2af7f9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodValue_7344dfa4-4ffd-47bf-be34-2ad2f24d6a2a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockRepurchasedDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodValue_7344dfa4-4ffd-47bf-be34-2ad2f24d6a2a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_4ac94e48-b6a2-43eb-9924-59c11e804808" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_4ac94e48-b6a2-43eb-9924-59c11e804808" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_a4f92f08-6e21-42b4-a3fe-2c2d9f7e6399" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_a4f92f08-6e21-42b4-a3fe-2c2d9f7e6399" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_5847496f-eae8-48a1-aa10-967e90b687b2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_5847496f-eae8-48a1-aa10-967e90b687b2" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_50bf0b12-fbe1-4035-9f5c-1a2c2842f6e7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_50bf0b12-fbe1-4035-9f5c-1a2c2842f6e7" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_74f6c0f1-1259-4650-9c0f-76a09e222646" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_74f6c0f1-1259-4650-9c0f-76a09e222646" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_70b02148-24b3-4e70-b4ee-066540173b35" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_70b02148-24b3-4e70-b4ee-066540173b35" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax_32e79445-7a43-490b-9f3b-69b99b29bc22" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax_32e79445-7a43-490b-9f3b-69b99b29bc22" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_CapitalContributionOnIssuanceOfConvertibleNoteRelatedParty_63d6877f-37f2-415c-bc12-eaf265bc437e" xlink:href="asan-20220131.xsd#asan_CapitalContributionOnIssuanceOfConvertibleNoteRelatedParty"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_asan_CapitalContributionOnIssuanceOfConvertibleNoteRelatedParty_63d6877f-37f2-415c-bc12-eaf265bc437e" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent_8551c970-cc9f-42c1-bd67-b0ed71892ee6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent_8551c970-cc9f-42c1-bd67-b0ed71892ee6" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_b8edb1e6-8804-4bfa-8f23-755b436143e1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6d069e08-57d3-4905-bd70-061b29d96462" xlink:to="loc_us-gaap_NetIncomeLoss_b8edb1e6-8804-4bfa-8f23-755b436143e1" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_716091a3-9ee4-4b00-a29d-5126e2ac5e76" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_86adb7a4-4e7b-417b-b813-a2e5ba8d524a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_572c7a1c-35f3-43e4-bfb0-721013b7ce98" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncreaseDecreaseInTemporaryEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_d9465dbc-baf5-4a15-a853-77461f9de73f" xlink:to="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_572c7a1c-35f3-43e4-bfb0-721013b7ce98" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesOutstanding_b4348de2-4b9c-4ae6-a4d5-53d55fd9d324" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TemporaryEquitySharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_572c7a1c-35f3-43e4-bfb0-721013b7ce98" xlink:to="loc_us-gaap_TemporaryEquitySharesOutstanding_b4348de2-4b9c-4ae6-a4d5-53d55fd9d324" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_0571c630-eec1-4b3c-ba84-3143b511de8e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TemporaryEquityCarryingAmountAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_572c7a1c-35f3-43e4-bfb0-721013b7ce98" xlink:to="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_0571c630-eec1-4b3c-ba84-3143b511de8e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_d644897c-57f4-4d21-b6fa-72909b3b2d98" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_572c7a1c-35f3-43e4-bfb0-721013b7ce98" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_d644897c-57f4-4d21-b6fa-72909b3b2d98" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationsOfTemporaryToPermanentEquity_5607c8bd-f2f6-4a5f-9253-8b383414718e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ReclassificationsOfTemporaryToPermanentEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_572c7a1c-35f3-43e4-bfb0-721013b7ce98" xlink:to="loc_us-gaap_ReclassificationsOfTemporaryToPermanentEquity_5607c8bd-f2f6-4a5f-9253-8b383414718e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesOutstanding_2e9f5115-807f-4ac5-bc82-11e2aacc9183" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TemporaryEquitySharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_10837307-9590-4fc7-9f9f-fa53303b4d50" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TemporaryEquityCarryingAmountAttributableToParent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_4d08eca5-56da-49ae-9393-d9c287c3bcb6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_d9465dbc-baf5-4a15-a853-77461f9de73f" xlink:to="loc_us-gaap_StatementTable_4d08eca5-56da-49ae-9393-d9c287c3bcb6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_2aad2005-3458-44ca-b211-86d6be7d80c6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_4d08eca5-56da-49ae-9393-d9c287c3bcb6" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_2aad2005-3458-44ca-b211-86d6be7d80c6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_2aad2005-3458-44ca-b211-86d6be7d80c6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_2aad2005-3458-44ca-b211-86d6be7d80c6" xlink:to="loc_us-gaap_EquityComponentDomain_2aad2005-3458-44ca-b211-86d6be7d80c6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_9dcff9f9-b388-42f9-9341-9e08b5a97c39" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_2aad2005-3458-44ca-b211-86d6be7d80c6" xlink:to="loc_us-gaap_EquityComponentDomain_9dcff9f9-b388-42f9-9341-9e08b5a97c39" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_808fdb7b-ec45-430c-9fdc-9e0f963a3d76" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_9dcff9f9-b388-42f9-9341-9e08b5a97c39" xlink:to="loc_us-gaap_CommonStockMember_808fdb7b-ec45-430c-9fdc-9e0f963a3d76" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_62418ab5-8366-455c-ac76-030f1ecdea0c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_9dcff9f9-b388-42f9-9341-9e08b5a97c39" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_62418ab5-8366-455c-ac76-030f1ecdea0c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_d8323946-beb3-41c6-bb34-a46aec8dafc6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_9dcff9f9-b388-42f9-9341-9e08b5a97c39" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_d8323946-beb3-41c6-bb34-a46aec8dafc6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_87bc335a-82ea-45f8-bdca-70db84c43997" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_9dcff9f9-b388-42f9-9341-9e08b5a97c39" xlink:to="loc_us-gaap_RetainedEarningsMember_87bc335a-82ea-45f8-bdca-70db84c43997" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_c0d1cfd8-69f9-4ef6-8fbb-a4027c66b588" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_4d08eca5-56da-49ae-9393-d9c287c3bcb6" xlink:to="loc_us-gaap_StatementClassOfStockAxis_c0d1cfd8-69f9-4ef6-8fbb-a4027c66b588" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_c0d1cfd8-69f9-4ef6-8fbb-a4027c66b588_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_c0d1cfd8-69f9-4ef6-8fbb-a4027c66b588" xlink:to="loc_us-gaap_ClassOfStockDomain_c0d1cfd8-69f9-4ef6-8fbb-a4027c66b588_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_b2af73a2-069d-4f74-bc29-f0ecd9121dcd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_c0d1cfd8-69f9-4ef6-8fbb-a4027c66b588" xlink:to="loc_us-gaap_ClassOfStockDomain_b2af73a2-069d-4f74-bc29-f0ecd9121dcd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RedeemableConvertiblePreferredStockMember_24764921-2643-4354-af30-e16117017d16" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RedeemableConvertiblePreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_b2af73a2-069d-4f74-bc29-f0ecd9121dcd" xlink:to="loc_us-gaap_RedeemableConvertiblePreferredStockMember_24764921-2643-4354-af30-e16117017d16" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesNarrativeDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesNarrativeDetails" xlink:type="extended" id="ia1e797800aa7453b864b9840385606ef_BasisofPresentationandSummaryofSignificantAccountingPoliciesNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfessionalFees_85c216ff-2851-40c2-9d52-c90a34157be2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProfessionalFees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:to="loc_us-gaap_ProfessionalFees_85c216ff-2851-40c2-9d52-c90a34157be2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_7e8ecfe0-7d82-4c11-a608-3383ef99c658" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_7e8ecfe0-7d82-4c11-a608-3383ef99c658" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdvertisingExpense_6577a91f-8339-490d-8eca-c419ee4c4d68" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdvertisingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:to="loc_us-gaap_AdvertisingExpense_6577a91f-8339-490d-8eca-c419ee4c4d68" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCash_7dc00785-35df-4fa2-9cd3-a3c953020782" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:to="loc_us-gaap_RestrictedCash_7dc00785-35df-4fa2-9cd3-a3c953020782" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTerm_6c027a0f-e467-4c8c-9844-e7331d5ac473" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTerm_6c027a0f-e467-4c8c-9844-e7331d5ac473" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MarketableSecuritiesRealizedGainLoss_f1346e6b-2e76-431c-90ce-73517cd7aef8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_MarketableSecuritiesRealizedGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:to="loc_us-gaap_MarketableSecuritiesRealizedGainLoss_f1346e6b-2e76-431c-90ce-73517cd7aef8" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_da2f7f1f-59ea-45da-b17b-dff835fa8402" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_da2f7f1f-59ea-45da-b17b-dff835fa8402" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_72033610-4d96-4b52-bba2-bc34f9db30de" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_72033610-4d96-4b52-bba2-bc34f9db30de" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairmentOfLongLivedAssetsHeldForUse_ab3ab713-0358-4487-bddc-c60d4cb6a5c2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ImpairmentOfLongLivedAssetsHeldForUse"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:to="loc_us-gaap_ImpairmentOfLongLivedAssetsHeldForUse_ab3ab713-0358-4487-bddc-c60d4cb6a5c2" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_b57edcb9-0991-4521-8d45-7d1c45caea6f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3bd2fb66-ad06-42ab-8cdc-3a1f75a3062c" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_b57edcb9-0991-4521-8d45-7d1c45caea6f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_0b6ca192-6377-4a5a-a62d-774fb3d7d1dc" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_b57edcb9-0991-4521-8d45-7d1c45caea6f" xlink:to="loc_srt_RangeAxis_0b6ca192-6377-4a5a-a62d-774fb3d7d1dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_0b6ca192-6377-4a5a-a62d-774fb3d7d1dc_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_0b6ca192-6377-4a5a-a62d-774fb3d7d1dc" xlink:to="loc_srt_RangeMember_0b6ca192-6377-4a5a-a62d-774fb3d7d1dc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_cb3ed089-4b98-4598-bd2a-9c2a82251063" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_0b6ca192-6377-4a5a-a62d-774fb3d7d1dc" xlink:to="loc_srt_RangeMember_cb3ed089-4b98-4598-bd2a-9c2a82251063" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_4ddb2070-6a42-4ad7-a352-dbd5b00b939f" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_cb3ed089-4b98-4598-bd2a-9c2a82251063" xlink:to="loc_srt_MinimumMember_4ddb2070-6a42-4ad7-a352-dbd5b00b939f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_2594f6cf-4297-477a-9e4f-648cb2d8cd24" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_cb3ed089-4b98-4598-bd2a-9c2a82251063" xlink:to="loc_srt_MaximumMember_2594f6cf-4297-477a-9e4f-648cb2d8cd24" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MajorCustomersAxis_2bb5d025-daa9-45b2-9171-0dd461c5ae1b" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_b57edcb9-0991-4521-8d45-7d1c45caea6f" xlink:to="loc_srt_MajorCustomersAxis_2bb5d025-daa9-45b2-9171-0dd461c5ae1b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_2bb5d025-daa9-45b2-9171-0dd461c5ae1b_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MajorCustomersAxis_2bb5d025-daa9-45b2-9171-0dd461c5ae1b" xlink:to="loc_srt_NameOfMajorCustomerDomain_2bb5d025-daa9-45b2-9171-0dd461c5ae1b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_b2fea5f3-edaa-4c8e-867f-a8295f515d98" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MajorCustomersAxis_2bb5d025-daa9-45b2-9171-0dd461c5ae1b" xlink:to="loc_srt_NameOfMajorCustomerDomain_b2fea5f3-edaa-4c8e-867f-a8295f515d98" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_CustomerOneMember_e88d3e33-8a27-4d8c-8357-d6abcd24fa58" xlink:href="asan-20220131.xsd#asan_CustomerOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_b2fea5f3-edaa-4c8e-867f-a8295f515d98" xlink:to="loc_asan_CustomerOneMember_e88d3e33-8a27-4d8c-8357-d6abcd24fa58" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_25cabc97-2092-4674-8c81-c2bc13051e8e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_b57edcb9-0991-4521-8d45-7d1c45caea6f" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_25cabc97-2092-4674-8c81-c2bc13051e8e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_25cabc97-2092-4674-8c81-c2bc13051e8e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_25cabc97-2092-4674-8c81-c2bc13051e8e" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_25cabc97-2092-4674-8c81-c2bc13051e8e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_ef1522d9-1642-4b23-abe1-58dae7c25ecc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_25cabc97-2092-4674-8c81-c2bc13051e8e" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_ef1522d9-1642-4b23-abe1-58dae7c25ecc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerMember_7b913089-99bd-4461-82ea-8185b3b61610" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueFromContractWithCustomerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_ef1522d9-1642-4b23-abe1-58dae7c25ecc" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerMember_7b913089-99bd-4461-82ea-8185b3b61610" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_785207af-deff-48cc-906a-3fe1f33f8710" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_b57edcb9-0991-4521-8d45-7d1c45caea6f" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_785207af-deff-48cc-906a-3fe1f33f8710" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_785207af-deff-48cc-906a-3fe1f33f8710_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_785207af-deff-48cc-906a-3fe1f33f8710" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_785207af-deff-48cc-906a-3fe1f33f8710_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_e7335d92-849e-4b86-b466-fd21c0a35208" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_785207af-deff-48cc-906a-3fe1f33f8710" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_e7335d92-849e-4b86-b466-fd21c0a35208" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerConcentrationRiskMember_ee2b4db1-2d10-4bda-9634-528adef234c1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CustomerConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_e7335d92-849e-4b86-b466-fd21c0a35208" xlink:to="loc_us-gaap_CustomerConcentrationRiskMember_ee2b4db1-2d10-4bda-9634-528adef234c1" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesPropertyAndEquipmentDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesPropertyAndEquipmentDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesPropertyAndEquipmentDetails" xlink:type="extended" id="i28eac741c6ef4ad2b47aea3b3f0fbc84_BasisofPresentationandSummaryofSignificantAccountingPoliciesPropertyAndEquipmentDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_29cb340c-b679-420a-a065-901172290a1b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_c9a3788e-5cf0-42c5-a23b-f42380ce2c1e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_29cb340c-b679-420a-a065-901172290a1b" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_c9a3788e-5cf0-42c5-a23b-f42380ce2c1e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_f4d12026-d278-4446-af99-1149c4d448c8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_29cb340c-b679-420a-a065-901172290a1b" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_f4d12026-d278-4446-af99-1149c4d448c8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_0e562735-9342-4e1d-942d-d4c8ac102264" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_f4d12026-d278-4446-af99-1149c4d448c8" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_0e562735-9342-4e1d-942d-d4c8ac102264" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_0e562735-9342-4e1d-942d-d4c8ac102264_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_0e562735-9342-4e1d-942d-d4c8ac102264" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_0e562735-9342-4e1d-942d-d4c8ac102264_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_3fd16e8e-506b-4863-804e-e2ec42c56b24" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_0e562735-9342-4e1d-942d-d4c8ac102264" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_3fd16e8e-506b-4863-804e-e2ec42c56b24" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerEquipmentMember_5ba7a90a-3378-4ab1-bbff-d04665786bd6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_3fd16e8e-506b-4863-804e-e2ec42c56b24" xlink:to="loc_us-gaap_ComputerEquipmentMember_5ba7a90a-3378-4ab1-bbff-d04665786bd6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_5338d377-3559-4569-835e-d90e9952ecdc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FurnitureAndFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_3fd16e8e-506b-4863-804e-e2ec42c56b24" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_5338d377-3559-4569-835e-d90e9952ecdc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SoftwareAndSoftwareDevelopmentCostsMember_112b2a14-5952-43bb-9870-5410fa7f9b57" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SoftwareAndSoftwareDevelopmentCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_3fd16e8e-506b-4863-804e-e2ec42c56b24" xlink:to="loc_us-gaap_SoftwareAndSoftwareDevelopmentCostsMember_112b2a14-5952-43bb-9870-5410fa7f9b57" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_1cc5fb1e-a6aa-4c80-93d8-46bef20073f1" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_f4d12026-d278-4446-af99-1149c4d448c8" xlink:to="loc_srt_RangeAxis_1cc5fb1e-a6aa-4c80-93d8-46bef20073f1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_1cc5fb1e-a6aa-4c80-93d8-46bef20073f1_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_1cc5fb1e-a6aa-4c80-93d8-46bef20073f1" xlink:to="loc_srt_RangeMember_1cc5fb1e-a6aa-4c80-93d8-46bef20073f1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_89360325-56a6-4bbd-ae3f-43eef388ead8" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_1cc5fb1e-a6aa-4c80-93d8-46bef20073f1" xlink:to="loc_srt_RangeMember_89360325-56a6-4bbd-ae3f-43eef388ead8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_db381b21-d234-47e9-a97a-8421cb64a07d" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_89360325-56a6-4bbd-ae3f-43eef388ead8" xlink:to="loc_srt_MinimumMember_db381b21-d234-47e9-a97a-8421cb64a07d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_3745b589-0d1f-4e9c-8944-755c1f4ecbaa" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_89360325-56a6-4bbd-ae3f-43eef388ead8" xlink:to="loc_srt_MaximumMember_3745b589-0d1f-4e9c-8944-755c1f4ecbaa" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/RevenuePerformanceObligationsDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#RevenuePerformanceObligationsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/RevenuePerformanceObligationsDetails" xlink:type="extended" id="i861943ba5eeb4dc8b7cb1107a8c1ee43_RevenuePerformanceObligationsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_28a1e83a-c4e3-464e-a25b-a56c0ce042e6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_692ee644-e0bf-4b39-966e-78bdc72fdfe4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_28a1e83a-c4e3-464e-a25b-a56c0ce042e6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_692ee644-e0bf-4b39-966e-78bdc72fdfe4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_81aefdfe-0144-45e5-bed3-cd2f423acfa7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_28a1e83a-c4e3-464e-a25b-a56c0ce042e6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_81aefdfe-0144-45e5-bed3-cd2f423acfa7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_4c3bbb42-bbf7-432b-b0d6-f342ae316964" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_28a1e83a-c4e3-464e-a25b-a56c0ce042e6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_4c3bbb42-bbf7-432b-b0d6-f342ae316964" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_2ad9ca56-1e39-494a-a906-0ac83436d33c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_28a1e83a-c4e3-464e-a25b-a56c0ce042e6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_2ad9ca56-1e39-494a-a906-0ac83436d33c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis_c033148d-8d3f-4fca-9045-2a703ded7a96" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_2ad9ca56-1e39-494a-a906-0ac83436d33c" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis_c033148d-8d3f-4fca-9045-2a703ded7a96" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/RevenuePerformanceObligationsDetails_1" xlink:type="simple" xlink:href="asan-20220131.xsd#RevenuePerformanceObligationsDetails_1"/>
  <link:definitionLink xlink:role="http://asana.com/role/RevenuePerformanceObligationsDetails_1" xlink:type="extended" id="ic8b25c7878bd4c0db36e7adf3b7fc913_RevenuePerformanceObligationsDetails_1">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_28a1e83a-c4e3-464e-a25b-a56c0ce042e6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_692ee644-e0bf-4b39-966e-78bdc72fdfe4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_28a1e83a-c4e3-464e-a25b-a56c0ce042e6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_692ee644-e0bf-4b39-966e-78bdc72fdfe4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_81aefdfe-0144-45e5-bed3-cd2f423acfa7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_28a1e83a-c4e3-464e-a25b-a56c0ce042e6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_81aefdfe-0144-45e5-bed3-cd2f423acfa7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_4c3bbb42-bbf7-432b-b0d6-f342ae316964" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_28a1e83a-c4e3-464e-a25b-a56c0ce042e6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_4c3bbb42-bbf7-432b-b0d6-f342ae316964" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_2ad9ca56-1e39-494a-a906-0ac83436d33c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_28a1e83a-c4e3-464e-a25b-a56c0ce042e6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_2ad9ca56-1e39-494a-a906-0ac83436d33c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/FairValueMeasurementsFairValueHierarchyDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#FairValueMeasurementsFairValueHierarchyDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/FairValueMeasurementsFairValueHierarchyDetails" xlink:type="extended" id="i71940cc02c1d4573912a83f8555c45b0_FairValueMeasurementsFairValueHierarchyDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d27a0d94-ba40-4936-a759-9a1acbcdef48" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_e517725a-52d4-4ad0-bd4a-ff4a9a4d349d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d27a0d94-ba40-4936-a759-9a1acbcdef48" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_e517725a-52d4-4ad0-bd4a-ff4a9a4d349d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_88c6d0ca-1d77-42d0-89dc-aab989420ca5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d27a0d94-ba40-4936-a759-9a1acbcdef48" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_88c6d0ca-1d77-42d0-89dc-aab989420ca5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsFairValueDisclosure_2f24ed82-84b1-43ad-ad69-81fb9a5e333b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherAssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d27a0d94-ba40-4936-a759-9a1acbcdef48" xlink:to="loc_us-gaap_OtherAssetsFairValueDisclosure_2f24ed82-84b1-43ad-ad69-81fb9a5e333b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_dcc0cd4f-c28b-426a-8773-ccce74e25baf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d27a0d94-ba40-4936-a759-9a1acbcdef48" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_dcc0cd4f-c28b-426a-8773-ccce74e25baf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_771fe34f-14b1-4fe5-b1f6-afd190f5a76e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d27a0d94-ba40-4936-a759-9a1acbcdef48" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_771fe34f-14b1-4fe5-b1f6-afd190f5a76e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_5311b38a-9a8a-4577-97a9-936fcdfe790b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_771fe34f-14b1-4fe5-b1f6-afd190f5a76e" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_5311b38a-9a8a-4577-97a9-936fcdfe790b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_5311b38a-9a8a-4577-97a9-936fcdfe790b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_5311b38a-9a8a-4577-97a9-936fcdfe790b" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_5311b38a-9a8a-4577-97a9-936fcdfe790b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_d43c0763-fd37-4789-b4e2-a0b92b089a44" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_5311b38a-9a8a-4577-97a9-936fcdfe790b" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_d43c0763-fd37-4789-b4e2-a0b92b089a44" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_1cda9ca2-7b76-4c8f-989b-696a330145b6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_d43c0763-fd37-4789-b4e2-a0b92b089a44" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_1cda9ca2-7b76-4c8f-989b-696a330145b6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_e2dd9195-9a07-4721-992c-2516943cd882" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_d43c0763-fd37-4789-b4e2-a0b92b089a44" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_e2dd9195-9a07-4721-992c-2516943cd882" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_65b3baae-b2bb-40b3-8dc2-517e651cd1af" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_d43c0763-fd37-4789-b4e2-a0b92b089a44" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_65b3baae-b2bb-40b3-8dc2-517e651cd1af" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_656685ed-e0a7-481f-bb05-a68dc3ccd74d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_771fe34f-14b1-4fe5-b1f6-afd190f5a76e" xlink:to="loc_us-gaap_FinancialInstrumentAxis_656685ed-e0a7-481f-bb05-a68dc3ccd74d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_656685ed-e0a7-481f-bb05-a68dc3ccd74d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_656685ed-e0a7-481f-bb05-a68dc3ccd74d" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_656685ed-e0a7-481f-bb05-a68dc3ccd74d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_6be09623-fe93-4ce0-ba4c-b1a83e3b613b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_656685ed-e0a7-481f-bb05-a68dc3ccd74d" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_6be09623-fe93-4ce0-ba4c-b1a83e3b613b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_a91d3435-7e26-46d6-91a5-47c4522a607c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_6be09623-fe93-4ce0-ba4c-b1a83e3b613b" xlink:to="loc_us-gaap_MoneyMarketFundsMember_a91d3435-7e26-46d6-91a5-47c4522a607c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember_11837c54-979a-419f-ad45-2c67d568a69b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_6be09623-fe93-4ce0-ba4c-b1a83e3b613b" xlink:to="loc_us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember_11837c54-979a-419f-ad45-2c67d568a69b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryAndGovernmentMember_23ed6fe1-a4e0-4d7d-8165-39f320114559" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USTreasuryAndGovernmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_6be09623-fe93-4ce0-ba4c-b1a83e3b613b" xlink:to="loc_us-gaap_USTreasuryAndGovernmentMember_23ed6fe1-a4e0-4d7d-8165-39f320114559" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_d5cccfd8-ddc1-4633-a95c-f1193a75e0f6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_6be09623-fe93-4ce0-ba4c-b1a83e3b613b" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_d5cccfd8-ddc1-4633-a95c-f1193a75e0f6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CertificatesOfDepositMember_bf42959b-a382-406e-9338-7f12cffcd553" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CertificatesOfDepositMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_6be09623-fe93-4ce0-ba4c-b1a83e3b613b" xlink:to="loc_us-gaap_CertificatesOfDepositMember_bf42959b-a382-406e-9338-7f12cffcd553" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/FairValueMeasurementsInvestmentsDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#FairValueMeasurementsInvestmentsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/FairValueMeasurementsInvestmentsDetails" xlink:type="extended" id="id2c46f4cdfb84d39a22926ab4b67ca9a_FairValueMeasurementsInvestmentsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_344d6971-c12d-41f2-9b0e-31684a5b2cbb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrentAbstract_f1563e8a-12ee-4004-a410-05c945c8b1d6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetsCurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_344d6971-c12d-41f2-9b0e-31684a5b2cbb" xlink:to="loc_us-gaap_AssetsCurrentAbstract_f1563e8a-12ee-4004-a410-05c945c8b1d6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_647886d0-9dc5-40b6-bd56-37eb7d111be2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_f1563e8a-12ee-4004-a410-05c945c8b1d6" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_647886d0-9dc5-40b6-bd56-37eb7d111be2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_7ab126c0-2bd4-429a-8414-f6035f36b189" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_f1563e8a-12ee-4004-a410-05c945c8b1d6" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_7ab126c0-2bd4-429a-8414-f6035f36b189" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_b435480e-c184-48ea-a741-69830447cc63" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_f1563e8a-12ee-4004-a410-05c945c8b1d6" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_b435480e-c184-48ea-a741-69830447cc63" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_226619b9-dc80-438b-87bf-1d1546f8c4ff" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_f1563e8a-12ee-4004-a410-05c945c8b1d6" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_226619b9-dc80-438b-87bf-1d1546f8c4ff" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermInvestmentsAbstract_0aa08054-6b29-4d75-9326-fc1e72426dbb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermInvestmentsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_344d6971-c12d-41f2-9b0e-31684a5b2cbb" xlink:to="loc_us-gaap_LongTermInvestmentsAbstract_0aa08054-6b29-4d75-9326-fc1e72426dbb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecurities_fccca991-cd35-4807-a70c-71f2a0a64149" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HeldToMaturitySecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_0aa08054-6b29-4d75-9326-fc1e72426dbb" xlink:to="loc_us-gaap_HeldToMaturitySecurities_fccca991-cd35-4807-a70c-71f2a0a64149" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain_044fa21c-330a-4fa2-a650-8ccad62b4bf1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_0aa08054-6b29-4d75-9326-fc1e72426dbb" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain_044fa21c-330a-4fa2-a650-8ccad62b4bf1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss_fafff638-1d04-4d07-a247-d6f5f743f8b1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_0aa08054-6b29-4d75-9326-fc1e72426dbb" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss_fafff638-1d04-4d07-a247-d6f5f743f8b1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesFairValue_0d589837-c6d4-40b5-8164-78f3343c7297" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HeldToMaturitySecuritiesFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_0aa08054-6b29-4d75-9326-fc1e72426dbb" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesFairValue_0d589837-c6d4-40b5-8164-78f3343c7297" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecurities_4ee064a3-7527-4379-934a-8dafd433578e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_344d6971-c12d-41f2-9b0e-31684a5b2cbb" xlink:to="loc_us-gaap_DebtSecurities_4ee064a3-7527-4379-934a-8dafd433578e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_DebtSecuritiesAccumulatedUnrealizedGains_3f413898-8088-44b1-9b0f-cc945f4168f5" xlink:href="asan-20220131.xsd#asan_DebtSecuritiesAccumulatedUnrealizedGains"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_344d6971-c12d-41f2-9b0e-31684a5b2cbb" xlink:to="loc_asan_DebtSecuritiesAccumulatedUnrealizedGains_3f413898-8088-44b1-9b0f-cc945f4168f5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_DebtSecuritiesAccumulatedUnrealizedLoss_cfe2721c-331c-49d6-9c63-2009e3ee1999" xlink:href="asan-20220131.xsd#asan_DebtSecuritiesAccumulatedUnrealizedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_344d6971-c12d-41f2-9b0e-31684a5b2cbb" xlink:to="loc_asan_DebtSecuritiesAccumulatedUnrealizedLoss_cfe2721c-331c-49d6-9c63-2009e3ee1999" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_DebtSecuritiesFairValue_c8d8a84b-b72f-4d47-93dd-dffb06900c02" xlink:href="asan-20220131.xsd#asan_DebtSecuritiesFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_344d6971-c12d-41f2-9b0e-31684a5b2cbb" xlink:to="loc_asan_DebtSecuritiesFairValue_c8d8a84b-b72f-4d47-93dd-dffb06900c02" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_ac94088d-4eb0-4bd6-b64b-40c96e888b65" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_344d6971-c12d-41f2-9b0e-31684a5b2cbb" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_ac94088d-4eb0-4bd6-b64b-40c96e888b65" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_b0cd1ce1-1050-4f43-9459-aa158e338358" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_ac94088d-4eb0-4bd6-b64b-40c96e888b65" xlink:to="loc_us-gaap_FinancialInstrumentAxis_b0cd1ce1-1050-4f43-9459-aa158e338358" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b0cd1ce1-1050-4f43-9459-aa158e338358_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_b0cd1ce1-1050-4f43-9459-aa158e338358" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b0cd1ce1-1050-4f43-9459-aa158e338358_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_1eb93159-4e2d-4744-a704-58a4f4a568d0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_b0cd1ce1-1050-4f43-9459-aa158e338358" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_1eb93159-4e2d-4744-a704-58a4f4a568d0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember_a1571a98-07b7-4b19-9372-a31802fc9e85" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_1eb93159-4e2d-4744-a704-58a4f4a568d0" xlink:to="loc_us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember_a1571a98-07b7-4b19-9372-a31802fc9e85" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryAndGovernmentMember_3e69dab8-009f-4a73-ac1d-8e18e0631991" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USTreasuryAndGovernmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_1eb93159-4e2d-4744-a704-58a4f4a568d0" xlink:to="loc_us-gaap_USTreasuryAndGovernmentMember_3e69dab8-009f-4a73-ac1d-8e18e0631991" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_aef586f5-0595-49f5-b113-4fabdcccea55" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_1eb93159-4e2d-4744-a704-58a4f4a568d0" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_aef586f5-0595-49f5-b113-4fabdcccea55" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CertificatesOfDepositMember_c86b204b-bdc2-4495-99dd-960493e776ee" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CertificatesOfDepositMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_1eb93159-4e2d-4744-a704-58a4f4a568d0" xlink:to="loc_us-gaap_CertificatesOfDepositMember_c86b204b-bdc2-4495-99dd-960493e776ee" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#FairValueMeasurementsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="extended" id="ic9caa1fa36c240a2ab4a36c8f9095914_FairValueMeasurementsNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_16042e3a-893e-4b87-8739-07d6f0979d15" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtFairValueDisclosures_980c0249-78a8-4f4a-a394-08aa91fa73f6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConvertibleDebtFairValueDisclosures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_16042e3a-893e-4b87-8739-07d6f0979d15" xlink:to="loc_us-gaap_ConvertibleDebtFairValueDisclosures_980c0249-78a8-4f4a-a394-08aa91fa73f6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtTerm_0fe1cbbc-ec83-4e9a-9f85-798e0a19257b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebtTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_16042e3a-893e-4b87-8739-07d6f0979d15" xlink:to="loc_us-gaap_LongTermDebtTerm_0fe1cbbc-ec83-4e9a-9f85-798e0a19257b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_0e58396d-a780-4b41-9ea3-8af5f277fef1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_16042e3a-893e-4b87-8739-07d6f0979d15" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_0e58396d-a780-4b41-9ea3-8af5f277fef1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSeniorLongTermDebt_bb4f21c0-1799-4855-b9e9-99ba0af85b5a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromIssuanceOfSeniorLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_16042e3a-893e-4b87-8739-07d6f0979d15" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSeniorLongTermDebt_bb4f21c0-1799-4855-b9e9-99ba0af85b5a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_5840ce96-d030-4dc9-8056-5311f49c781b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_16042e3a-893e-4b87-8739-07d6f0979d15" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_5840ce96-d030-4dc9-8056-5311f49c781b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_6be58f67-d4d0-4bf3-b49d-62ded6527f51" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_16042e3a-893e-4b87-8739-07d6f0979d15" xlink:to="loc_us-gaap_DebtInstrumentTable_6be58f67-d4d0-4bf3-b49d-62ded6527f51" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_51f12560-fe80-4054-809a-8e4ba9525bdb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6be58f67-d4d0-4bf3-b49d-62ded6527f51" xlink:to="loc_us-gaap_DebtInstrumentAxis_51f12560-fe80-4054-809a-8e4ba9525bdb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_51f12560-fe80-4054-809a-8e4ba9525bdb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_51f12560-fe80-4054-809a-8e4ba9525bdb" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_51f12560-fe80-4054-809a-8e4ba9525bdb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_00b3f9e6-048f-46d2-89be-c57ed112ce9d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_51f12560-fe80-4054-809a-8e4ba9525bdb" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_00b3f9e6-048f-46d2-89be-c57ed112ce9d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_January2020ConvertibleNoteMember_0cf40c0b-3656-4d02-bd9e-15123dd0a069" xlink:href="asan-20220131.xsd#asan_January2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_00b3f9e6-048f-46d2-89be-c57ed112ce9d" xlink:to="loc_asan_January2020ConvertibleNoteMember_0cf40c0b-3656-4d02-bd9e-15123dd0a069" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_June2020ConvertibleNoteMember_c4c90ae2-e564-490c-9597-30e646a19aa9" xlink:href="asan-20220131.xsd#asan_June2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_00b3f9e6-048f-46d2-89be-c57ed112ce9d" xlink:to="loc_asan_June2020ConvertibleNoteMember_c4c90ae2-e564-490c-9597-30e646a19aa9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_TermLoanAgreementMember_9fa1babd-aa26-43a3-a126-d0cac621034d" xlink:href="asan-20220131.xsd#asan_TermLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_00b3f9e6-048f-46d2-89be-c57ed112ce9d" xlink:to="loc_asan_TermLoanAgreementMember_9fa1babd-aa26-43a3-a126-d0cac621034d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_0e17f7d4-7898-4e7a-9181-21e53634b833" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6be58f67-d4d0-4bf3-b49d-62ded6527f51" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_0e17f7d4-7898-4e7a-9181-21e53634b833" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_0e17f7d4-7898-4e7a-9181-21e53634b833_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_0e17f7d4-7898-4e7a-9181-21e53634b833" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_0e17f7d4-7898-4e7a-9181-21e53634b833_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_5d51e839-d0d4-49d8-b119-916203ac0ea7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_0e17f7d4-7898-4e7a-9181-21e53634b833" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_5d51e839-d0d4-49d8-b119-916203ac0ea7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_c8e5e8ca-d42e-4013-87cb-911bd5f87af4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_5d51e839-d0d4-49d8-b119-916203ac0ea7" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_c8e5e8ca-d42e-4013-87cb-911bd5f87af4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_90a1f35f-3246-4615-a1bc-44adb5766a4b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_5d51e839-d0d4-49d8-b119-916203ac0ea7" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_90a1f35f-3246-4615-a1bc-44adb5766a4b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_9a16de88-135b-48aa-91e9-4dd55d87b189" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6be58f67-d4d0-4bf3-b49d-62ded6527f51" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_9a16de88-135b-48aa-91e9-4dd55d87b189" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_9a16de88-135b-48aa-91e9-4dd55d87b189_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_9a16de88-135b-48aa-91e9-4dd55d87b189" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_9a16de88-135b-48aa-91e9-4dd55d87b189_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_76811021-cbb9-4bea-b348-84e2f62b36de" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_9a16de88-135b-48aa-91e9-4dd55d87b189" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_76811021-cbb9-4bea-b348-84e2f62b36de" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_b9207255-9f26-4e9c-b7f9-8cf7ad4ef25c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_76811021-cbb9-4bea-b348-84e2f62b36de" xlink:to="loc_us-gaap_SecuredDebtMember_b9207255-9f26-4e9c-b7f9-8cf7ad4ef25c" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/BalanceSheetComponentsPropertyandEquipmentDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#BalanceSheetComponentsPropertyandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BalanceSheetComponentsPropertyandEquipmentDetails" xlink:type="extended" id="ida3a2d39f2e3430b8cd5e49275100123_BalanceSheetComponentsPropertyandEquipmentDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7254494d-ae6b-4340-b3b9-38e281b773ec" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_d03a4a44-1db2-4c8c-95f2-1c58f942eb66" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7254494d-ae6b-4340-b3b9-38e281b773ec" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_d03a4a44-1db2-4c8c-95f2-1c58f942eb66" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_12008268-b1d4-4310-ba9c-de50436c06ea" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7254494d-ae6b-4340-b3b9-38e281b773ec" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_12008268-b1d4-4310-ba9c-de50436c06ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_65037f2d-9d77-4c0d-a0e5-a4ac0255ac9c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7254494d-ae6b-4340-b3b9-38e281b773ec" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_65037f2d-9d77-4c0d-a0e5-a4ac0255ac9c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_9a585b63-36b8-49d6-af8d-4c381533cd32" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7254494d-ae6b-4340-b3b9-38e281b773ec" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_9a585b63-36b8-49d6-af8d-4c381533cd32" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_5b3f9acb-f9a0-4c33-97c4-997f26f963d0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7254494d-ae6b-4340-b3b9-38e281b773ec" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_5b3f9acb-f9a0-4c33-97c4-997f26f963d0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_05f98184-a76c-4229-b46e-c5ddbf27d444" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_5b3f9acb-f9a0-4c33-97c4-997f26f963d0" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_05f98184-a76c-4229-b46e-c5ddbf27d444" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_05f98184-a76c-4229-b46e-c5ddbf27d444_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_05f98184-a76c-4229-b46e-c5ddbf27d444" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_05f98184-a76c-4229-b46e-c5ddbf27d444_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_ed6b610c-d76b-4045-8ffb-ea7e3b6d2f2c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_05f98184-a76c-4229-b46e-c5ddbf27d444" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_ed6b610c-d76b-4045-8ffb-ea7e3b6d2f2c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerEquipmentMember_bd33b36f-a891-4f4c-8d3a-915850674ed9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_ed6b610c-d76b-4045-8ffb-ea7e3b6d2f2c" xlink:to="loc_us-gaap_ComputerEquipmentMember_bd33b36f-a891-4f4c-8d3a-915850674ed9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_eab2bd2f-8313-4d83-a60e-4a6995b47a10" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FurnitureAndFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_ed6b610c-d76b-4045-8ffb-ea7e3b6d2f2c" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_eab2bd2f-8313-4d83-a60e-4a6995b47a10" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_10b85f80-a5fe-41de-9ab9-048b56fe7a53" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_ed6b610c-d76b-4045-8ffb-ea7e3b6d2f2c" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_10b85f80-a5fe-41de-9ab9-048b56fe7a53" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SoftwareAndSoftwareDevelopmentCostsMember_3a054ece-4ca2-42c4-8ec8-b2197adec6b1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SoftwareAndSoftwareDevelopmentCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_ed6b610c-d76b-4045-8ffb-ea7e3b6d2f2c" xlink:to="loc_us-gaap_SoftwareAndSoftwareDevelopmentCostsMember_3a054ece-4ca2-42c4-8ec8-b2197adec6b1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConstructionInProgressMember_b80b3e0b-63b4-47e2-a66c-b86e526c93f5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConstructionInProgressMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_ed6b610c-d76b-4045-8ffb-ea7e3b6d2f2c" xlink:to="loc_us-gaap_ConstructionInProgressMember_b80b3e0b-63b4-47e2-a66c-b86e526c93f5" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/ConvertibleNotesRelatedPartyNarrativeDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#ConvertibleNotesRelatedPartyNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConvertibleNotesRelatedPartyNarrativeDetails" xlink:type="extended" id="i7bf20af1e9a44a399b70436dbf5da8a9_ConvertibleNotesRelatedPartyNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_010b1328-4faf-4645-8b46-08d3f36a03c6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_4f2343c8-9333-4c32-bade-bb00a18fee4c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_010b1328-4faf-4645-8b46-08d3f36a03c6" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_4f2343c8-9333-4c32-bade-bb00a18fee4c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_54f161b5-11b7-4d39-b038-83d1950036bc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_010b1328-4faf-4645-8b46-08d3f36a03c6" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_54f161b5-11b7-4d39-b038-83d1950036bc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_0fa6a272-143c-4c66-96fe-4d6d6aff79b1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_010b1328-4faf-4645-8b46-08d3f36a03c6" xlink:to="loc_us-gaap_LongTermDebt_0fa6a272-143c-4c66-96fe-4d6d6aff79b1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_NumberOfDebtInstrumentsIssuedInThePeriod_ac7039af-602a-4518-b342-993df650fcbe" xlink:href="asan-20220131.xsd#asan_NumberOfDebtInstrumentsIssuedInThePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_010b1328-4faf-4645-8b46-08d3f36a03c6" xlink:to="loc_asan_NumberOfDebtInstrumentsIssuedInThePeriod_ac7039af-602a-4518-b342-993df650fcbe" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtTerm_abaf6272-7401-4579-90f0-1362e71fc2cf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebtTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_010b1328-4faf-4645-8b46-08d3f36a03c6" xlink:to="loc_us-gaap_LongTermDebtTerm_abaf6272-7401-4579-90f0-1362e71fc2cf" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_5b94377e-0cb4-4523-a6a5-8321685d16b1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_010b1328-4faf-4645-8b46-08d3f36a03c6" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_5b94377e-0cb4-4523-a6a5-8321685d16b1" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestPayableCurrentAndNoncurrent_dd89c568-80c7-4a7b-aae5-be36d94ab13a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestPayableCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_010b1328-4faf-4645-8b46-08d3f36a03c6" xlink:to="loc_us-gaap_InterestPayableCurrentAndNoncurrent_dd89c568-80c7-4a7b-aae5-be36d94ab13a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnamortizedDiscount_8f6a9797-cac6-4c22-bed6-a31ce52393ed" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentUnamortizedDiscount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_010b1328-4faf-4645-8b46-08d3f36a03c6" xlink:to="loc_us-gaap_DebtInstrumentUnamortizedDiscount_8f6a9797-cac6-4c22-bed6-a31ce52393ed" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_28f4fcc6-3d4f-4af6-9a8b-d9cd9bfa5376" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_010b1328-4faf-4645-8b46-08d3f36a03c6" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_28f4fcc6-3d4f-4af6-9a8b-d9cd9bfa5376" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_cfd93d20-8fd6-4f66-9517-6cd012b2ade7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_28f4fcc6-3d4f-4af6-9a8b-d9cd9bfa5376" xlink:to="loc_us-gaap_DebtInstrumentAxis_cfd93d20-8fd6-4f66-9517-6cd012b2ade7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_cfd93d20-8fd6-4f66-9517-6cd012b2ade7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_cfd93d20-8fd6-4f66-9517-6cd012b2ade7" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_cfd93d20-8fd6-4f66-9517-6cd012b2ade7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_e0bbd66e-bee4-4463-bba4-7ea1204e54ec" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_cfd93d20-8fd6-4f66-9517-6cd012b2ade7" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_e0bbd66e-bee4-4463-bba4-7ea1204e54ec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_January2020ConvertibleNoteMember_48fbacd1-d226-46a8-9cdb-062d88841092" xlink:href="asan-20220131.xsd#asan_January2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_e0bbd66e-bee4-4463-bba4-7ea1204e54ec" xlink:to="loc_asan_January2020ConvertibleNoteMember_48fbacd1-d226-46a8-9cdb-062d88841092" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_June2020ConvertibleNoteMember_5109bd9d-1212-4b8c-80cf-f5493de3d544" xlink:href="asan-20220131.xsd#asan_June2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_e0bbd66e-bee4-4463-bba4-7ea1204e54ec" xlink:to="loc_asan_June2020ConvertibleNoteMember_5109bd9d-1212-4b8c-80cf-f5493de3d544" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_TermLoanAgreementMember_31c48e64-0a3d-4682-92ad-83b978b7de1e" xlink:href="asan-20220131.xsd#asan_TermLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_e0bbd66e-bee4-4463-bba4-7ea1204e54ec" xlink:to="loc_asan_TermLoanAgreementMember_31c48e64-0a3d-4682-92ad-83b978b7de1e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_1060afe8-710e-4c7a-b3fd-bc62f08581ed" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_28f4fcc6-3d4f-4af6-9a8b-d9cd9bfa5376" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_1060afe8-710e-4c7a-b3fd-bc62f08581ed" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_1060afe8-710e-4c7a-b3fd-bc62f08581ed_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_1060afe8-710e-4c7a-b3fd-bc62f08581ed" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_1060afe8-710e-4c7a-b3fd-bc62f08581ed_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_370e9e84-670c-454c-9223-e19d9e90d016" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_1060afe8-710e-4c7a-b3fd-bc62f08581ed" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_370e9e84-670c-454c-9223-e19d9e90d016" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtMember_4cbe87e1-189d-4b85-9de7-d6b7acb53670" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConvertibleDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_370e9e84-670c-454c-9223-e19d9e90d016" xlink:to="loc_us-gaap_ConvertibleDebtMember_4cbe87e1-189d-4b85-9de7-d6b7acb53670" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_74eeb64e-08fb-45ee-a544-22b5457b39d3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_370e9e84-670c-454c-9223-e19d9e90d016" xlink:to="loc_us-gaap_SecuredDebtMember_74eeb64e-08fb-45ee-a544-22b5457b39d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_e46bfd8c-594f-4556-82a0-b5f021d25a4c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_28f4fcc6-3d4f-4af6-9a8b-d9cd9bfa5376" xlink:to="loc_us-gaap_StatementClassOfStockAxis_e46bfd8c-594f-4556-82a0-b5f021d25a4c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_e46bfd8c-594f-4556-82a0-b5f021d25a4c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_e46bfd8c-594f-4556-82a0-b5f021d25a4c" xlink:to="loc_us-gaap_ClassOfStockDomain_e46bfd8c-594f-4556-82a0-b5f021d25a4c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_99bb1302-37c5-4712-8359-e056c2855e18" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_e46bfd8c-594f-4556-82a0-b5f021d25a4c" xlink:to="loc_us-gaap_ClassOfStockDomain_99bb1302-37c5-4712-8359-e056c2855e18" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_03b1e87e-c82e-40e3-8c18-efb84b6e7af8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_99bb1302-37c5-4712-8359-e056c2855e18" xlink:to="loc_us-gaap_CommonClassBMember_03b1e87e-c82e-40e3-8c18-efb84b6e7af8" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/ConvertibleNotesRelatedPartyNetCarryingAmountofConvertibleNotesDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#ConvertibleNotesRelatedPartyNetCarryingAmountofConvertibleNotesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConvertibleNotesRelatedPartyNetCarryingAmountofConvertibleNotesDetails" xlink:type="extended" id="i66b0a22f6b98485695abd7ea8e9c81d3_ConvertibleNotesRelatedPartyNetCarryingAmountofConvertibleNotesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_b899b71c-ec8a-4e01-a247-2f9f0427926e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_5eabca04-6120-4034-80a7-0cc05421ff32" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b899b71c-ec8a-4e01-a247-2f9f0427926e" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_5eabca04-6120-4034-80a7-0cc05421ff32" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnamortizedDiscount_6f07ee70-bff4-4355-b511-16f440458016" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentUnamortizedDiscount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b899b71c-ec8a-4e01-a247-2f9f0427926e" xlink:to="loc_us-gaap_DebtInstrumentUnamortizedDiscount_6f07ee70-bff4-4355-b511-16f440458016" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestPayableCurrentAndNoncurrent_13325443-227b-4ef2-babf-19d5280dfe2f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestPayableCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b899b71c-ec8a-4e01-a247-2f9f0427926e" xlink:to="loc_us-gaap_InterestPayableCurrentAndNoncurrent_13325443-227b-4ef2-babf-19d5280dfe2f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_3bbb18a4-9c7b-4501-b689-a5710f18b69e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b899b71c-ec8a-4e01-a247-2f9f0427926e" xlink:to="loc_us-gaap_LongTermDebt_3bbb18a4-9c7b-4501-b689-a5710f18b69e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_fb761382-f8e1-444d-90d0-16072b2df701" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b899b71c-ec8a-4e01-a247-2f9f0427926e" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_fb761382-f8e1-444d-90d0-16072b2df701" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_b1d7c2b4-156d-44bd-a32c-32fac78b7f4c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_fb761382-f8e1-444d-90d0-16072b2df701" xlink:to="loc_us-gaap_DebtInstrumentAxis_b1d7c2b4-156d-44bd-a32c-32fac78b7f4c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_b1d7c2b4-156d-44bd-a32c-32fac78b7f4c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_b1d7c2b4-156d-44bd-a32c-32fac78b7f4c" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_b1d7c2b4-156d-44bd-a32c-32fac78b7f4c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_2e7741e7-cb85-459d-bc87-0190692d5c64" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_b1d7c2b4-156d-44bd-a32c-32fac78b7f4c" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_2e7741e7-cb85-459d-bc87-0190692d5c64" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_January2020ConvertibleNoteMember_ad0e1a6d-b6f1-4a25-a912-a7978185e8e9" xlink:href="asan-20220131.xsd#asan_January2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_2e7741e7-cb85-459d-bc87-0190692d5c64" xlink:to="loc_asan_January2020ConvertibleNoteMember_ad0e1a6d-b6f1-4a25-a912-a7978185e8e9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_June2020ConvertibleNoteMember_0287fa57-501a-4e4d-b89b-0e86f77b4df3" xlink:href="asan-20220131.xsd#asan_June2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_2e7741e7-cb85-459d-bc87-0190692d5c64" xlink:to="loc_asan_June2020ConvertibleNoteMember_0287fa57-501a-4e4d-b89b-0e86f77b4df3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_1d19519f-3eb8-464d-b40d-6eef5e3bd641" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_fb761382-f8e1-444d-90d0-16072b2df701" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_1d19519f-3eb8-464d-b40d-6eef5e3bd641" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_1d19519f-3eb8-464d-b40d-6eef5e3bd641_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_1d19519f-3eb8-464d-b40d-6eef5e3bd641" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_1d19519f-3eb8-464d-b40d-6eef5e3bd641_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_514727ce-6346-40aa-a288-0cc2b8f4c005" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_1d19519f-3eb8-464d-b40d-6eef5e3bd641" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_514727ce-6346-40aa-a288-0cc2b8f4c005" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtMember_16f46f08-7a43-44b0-9fc8-f25e83646cf0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConvertibleDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_514727ce-6346-40aa-a288-0cc2b8f4c005" xlink:to="loc_us-gaap_ConvertibleDebtMember_16f46f08-7a43-44b0-9fc8-f25e83646cf0" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/ConvertibleNotesRelatedPartyInterestExpenseDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#ConvertibleNotesRelatedPartyInterestExpenseDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConvertibleNotesRelatedPartyInterestExpenseDetails" xlink:type="extended" id="i32bf7116703f412e9cc754f7ac2f2384_ConvertibleNotesRelatedPartyInterestExpenseDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_ea31b2f3-cd5d-4cfb-949f-3c3c92f5cdba" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfDebtDiscountPremium_16902da0-b0a2-41e9-8726-f9440b24f1f8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AmortizationOfDebtDiscountPremium"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_ea31b2f3-cd5d-4cfb-949f-3c3c92f5cdba" xlink:to="loc_us-gaap_AmortizationOfDebtDiscountPremium_16902da0-b0a2-41e9-8726-f9440b24f1f8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDebtExcludingAmortization_3f1377f3-4f76-45bd-9670-2b2d688c49e9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpenseDebtExcludingAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_ea31b2f3-cd5d-4cfb-949f-3c3c92f5cdba" xlink:to="loc_us-gaap_InterestExpenseDebtExcludingAmortization_3f1377f3-4f76-45bd-9670-2b2d688c49e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDebt_b6189e3c-e8d5-4e55-ba7d-2103b8bb81f7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpenseDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_ea31b2f3-cd5d-4cfb-949f-3c3c92f5cdba" xlink:to="loc_us-gaap_InterestExpenseDebt_b6189e3c-e8d5-4e55-ba7d-2103b8bb81f7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_afc70f87-44d7-44d1-a874-f8b456fdde6f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_ea31b2f3-cd5d-4cfb-949f-3c3c92f5cdba" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_afc70f87-44d7-44d1-a874-f8b456fdde6f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_3f23bd33-2a6b-43e5-84ad-b12f08ef0f40" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_afc70f87-44d7-44d1-a874-f8b456fdde6f" xlink:to="loc_us-gaap_DebtInstrumentAxis_3f23bd33-2a6b-43e5-84ad-b12f08ef0f40" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_3f23bd33-2a6b-43e5-84ad-b12f08ef0f40_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_3f23bd33-2a6b-43e5-84ad-b12f08ef0f40" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_3f23bd33-2a6b-43e5-84ad-b12f08ef0f40_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_9d1a132b-4d04-4455-a466-005c89b6181f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_3f23bd33-2a6b-43e5-84ad-b12f08ef0f40" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_9d1a132b-4d04-4455-a466-005c89b6181f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleNotesPayableMember_27415a89-23df-4069-a4b0-c6c25856fe4a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConvertibleNotesPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9d1a132b-4d04-4455-a466-005c89b6181f" xlink:to="loc_us-gaap_ConvertibleNotesPayableMember_27415a89-23df-4069-a4b0-c6c25856fe4a" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/DebtNarrativeDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#DebtNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/DebtNarrativeDetails" xlink:type="extended" id="id6be8ab81f90484e96aeb8c1d1bb6f0a_DebtNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_15b9fa5d-f43d-4a7c-ac01-6bce6622b209" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtTerm_0c6364bb-544f-4dff-8339-e5e906c466f1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebtTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_15b9fa5d-f43d-4a7c-ac01-6bce6622b209" xlink:to="loc_us-gaap_LongTermDebtTerm_0c6364bb-544f-4dff-8339-e5e906c466f1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_8ee35dc7-d435-402b-8755-0c786b94bfd5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_15b9fa5d-f43d-4a7c-ac01-6bce6622b209" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_8ee35dc7-d435-402b-8755-0c786b94bfd5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_a14f922f-58ba-4d43-96b8-dbd136d515da" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_15b9fa5d-f43d-4a7c-ac01-6bce6622b209" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_a14f922f-58ba-4d43-96b8-dbd136d515da" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_DebtInstrumentCovenantTermsCashBalanceRequirementAmount_fe0fa86e-76d4-4106-a304-9880bbbd2f57" xlink:href="asan-20220131.xsd#asan_DebtInstrumentCovenantTermsCashBalanceRequirementAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_15b9fa5d-f43d-4a7c-ac01-6bce6622b209" xlink:to="loc_asan_DebtInstrumentCovenantTermsCashBalanceRequirementAmount_fe0fa86e-76d4-4106-a304-9880bbbd2f57" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_8c8a714f-75d5-42fc-a924-256ef88ccbc3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_15b9fa5d-f43d-4a7c-ac01-6bce6622b209" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_8c8a714f-75d5-42fc-a924-256ef88ccbc3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_57ee6778-d8af-4a37-8613-8f519a263a3f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_15b9fa5d-f43d-4a7c-ac01-6bce6622b209" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_57ee6778-d8af-4a37-8613-8f519a263a3f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_d9c912e3-eb72-486c-a2c9-b89c6aad6ddd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_57ee6778-d8af-4a37-8613-8f519a263a3f" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_d9c912e3-eb72-486c-a2c9-b89c6aad6ddd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_d9c912e3-eb72-486c-a2c9-b89c6aad6ddd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_d9c912e3-eb72-486c-a2c9-b89c6aad6ddd" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_d9c912e3-eb72-486c-a2c9-b89c6aad6ddd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_1857c881-8c7a-49c3-803d-faa748f894aa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_d9c912e3-eb72-486c-a2c9-b89c6aad6ddd" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_1857c881-8c7a-49c3-803d-faa748f894aa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_7e8fa962-8c0d-4339-a151-72190727a631" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_1857c881-8c7a-49c3-803d-faa748f894aa" xlink:to="loc_us-gaap_SecuredDebtMember_7e8fa962-8c0d-4339-a151-72190727a631" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_71fe0e10-ca2d-4e5c-ba10-d527e3bdf111" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_57ee6778-d8af-4a37-8613-8f519a263a3f" xlink:to="loc_us-gaap_DebtInstrumentAxis_71fe0e10-ca2d-4e5c-ba10-d527e3bdf111" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_71fe0e10-ca2d-4e5c-ba10-d527e3bdf111_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_71fe0e10-ca2d-4e5c-ba10-d527e3bdf111" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_71fe0e10-ca2d-4e5c-ba10-d527e3bdf111_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_de3c662d-92b4-4ca4-97da-4e26310734c6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_71fe0e10-ca2d-4e5c-ba10-d527e3bdf111" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_de3c662d-92b4-4ca4-97da-4e26310734c6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_TermLoanAgreementMember_2198e904-0157-40de-b05b-d80d7a331b49" xlink:href="asan-20220131.xsd#asan_TermLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_de3c662d-92b4-4ca4-97da-4e26310734c6" xlink:to="loc_asan_TermLoanAgreementMember_2198e904-0157-40de-b05b-d80d7a331b49" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_9e7674e7-e0d6-4d83-962e-3953dbfae64d" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_57ee6778-d8af-4a37-8613-8f519a263a3f" xlink:to="loc_srt_RangeAxis_9e7674e7-e0d6-4d83-962e-3953dbfae64d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_9e7674e7-e0d6-4d83-962e-3953dbfae64d_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_9e7674e7-e0d6-4d83-962e-3953dbfae64d" xlink:to="loc_srt_RangeMember_9e7674e7-e0d6-4d83-962e-3953dbfae64d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_bb71d1db-086a-409d-aeec-80448d220e03" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_9e7674e7-e0d6-4d83-962e-3953dbfae64d" xlink:to="loc_srt_RangeMember_bb71d1db-086a-409d-aeec-80448d220e03" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_3298d3d2-b8c8-4b59-a8fa-f83bd4e2e00a" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_bb71d1db-086a-409d-aeec-80448d220e03" xlink:to="loc_srt_MinimumMember_3298d3d2-b8c8-4b59-a8fa-f83bd4e2e00a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_866136f7-f35a-441c-8dd7-473e784385b6" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_bb71d1db-086a-409d-aeec-80448d220e03" xlink:to="loc_srt_MaximumMember_866136f7-f35a-441c-8dd7-473e784385b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_ac7e477a-e3f1-4198-872a-73c9a6e9725d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_57ee6778-d8af-4a37-8613-8f519a263a3f" xlink:to="loc_us-gaap_VariableRateAxis_ac7e477a-e3f1-4198-872a-73c9a6e9725d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_ac7e477a-e3f1-4198-872a-73c9a6e9725d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_ac7e477a-e3f1-4198-872a-73c9a6e9725d" xlink:to="loc_us-gaap_VariableRateDomain_ac7e477a-e3f1-4198-872a-73c9a6e9725d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_72f9624d-8465-40f7-b895-41f434209046" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_ac7e477a-e3f1-4198-872a-73c9a6e9725d" xlink:to="loc_us-gaap_VariableRateDomain_72f9624d-8465-40f7-b895-41f434209046" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrimeRateMember_707a17db-9dc6-4993-95fd-c120eb828d26" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PrimeRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_72f9624d-8465-40f7-b895-41f434209046" xlink:to="loc_us-gaap_PrimeRateMember_707a17db-9dc6-4993-95fd-c120eb828d26" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_d6fd4f97-11f3-4534-a6b8-71ad93cf115e" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_57ee6778-d8af-4a37-8613-8f519a263a3f" xlink:to="loc_srt_StatementScenarioAxis_d6fd4f97-11f3-4534-a6b8-71ad93cf115e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_d6fd4f97-11f3-4534-a6b8-71ad93cf115e_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_d6fd4f97-11f3-4534-a6b8-71ad93cf115e" xlink:to="loc_srt_ScenarioUnspecifiedDomain_d6fd4f97-11f3-4534-a6b8-71ad93cf115e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_f608d916-caae-4ea2-a46d-511a3129543c" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_d6fd4f97-11f3-4534-a6b8-71ad93cf115e" xlink:to="loc_srt_ScenarioUnspecifiedDomain_f608d916-caae-4ea2-a46d-511a3129543c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_UnrestrictedCashBalanceTierOneMember_72d1a36b-3dd6-4215-9b35-8efdd6379072" xlink:href="asan-20220131.xsd#asan_UnrestrictedCashBalanceTierOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_f608d916-caae-4ea2-a46d-511a3129543c" xlink:to="loc_asan_UnrestrictedCashBalanceTierOneMember_72d1a36b-3dd6-4215-9b35-8efdd6379072" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_UnrestrictedCashBalanceTierTwoMember_3743cc37-4604-42c5-ab2e-8f2e30abca3f" xlink:href="asan-20220131.xsd#asan_UnrestrictedCashBalanceTierTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_f608d916-caae-4ea2-a46d-511a3129543c" xlink:to="loc_asan_UnrestrictedCashBalanceTierTwoMember_3743cc37-4604-42c5-ab2e-8f2e30abca3f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_UnrestrictedCashBalanceTierThreeMember_915155e1-f589-4aca-afc8-2eeb791e86b7" xlink:href="asan-20220131.xsd#asan_UnrestrictedCashBalanceTierThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_f608d916-caae-4ea2-a46d-511a3129543c" xlink:to="loc_asan_UnrestrictedCashBalanceTierThreeMember_915155e1-f589-4aca-afc8-2eeb791e86b7" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/DebtNetCarryingAmountofTermLoanDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#DebtNetCarryingAmountofTermLoanDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/DebtNetCarryingAmountofTermLoanDetails" xlink:type="extended" id="i4513a15557314e62ac5cb896f1ebf519_DebtNetCarryingAmountofTermLoanDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_af567f6d-7da3-412c-afc9-4f99e1cc54b8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_578483bc-9788-495f-87f5-8c16082bdbd9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_af567f6d-7da3-412c-afc9-4f99e1cc54b8" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_578483bc-9788-495f-87f5-8c16082bdbd9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestPayableCurrentAndNoncurrent_2cacbcff-0a35-40b6-a5ce-b565df25bd92" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestPayableCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_af567f6d-7da3-412c-afc9-4f99e1cc54b8" xlink:to="loc_us-gaap_InterestPayableCurrentAndNoncurrent_2cacbcff-0a35-40b6-a5ce-b565df25bd92" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredFinanceCostsNet_d2c2fb3f-6256-4fd2-9f20-e9116b5190c4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DeferredFinanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_af567f6d-7da3-412c-afc9-4f99e1cc54b8" xlink:to="loc_us-gaap_DeferredFinanceCostsNet_d2c2fb3f-6256-4fd2-9f20-e9116b5190c4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_ee5e84ae-d5cd-4957-b4b8-9706423186b6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_af567f6d-7da3-412c-afc9-4f99e1cc54b8" xlink:to="loc_us-gaap_LongTermDebt_ee5e84ae-d5cd-4957-b4b8-9706423186b6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtCurrent_c34edf16-afbc-48c6-8cc9-93f2de9525c1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebtCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_af567f6d-7da3-412c-afc9-4f99e1cc54b8" xlink:to="loc_us-gaap_LongTermDebtCurrent_c34edf16-afbc-48c6-8cc9-93f2de9525c1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtNoncurrent_93e33c59-dc8e-46c8-ab2c-a4bfffd73a52" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebtNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_af567f6d-7da3-412c-afc9-4f99e1cc54b8" xlink:to="loc_us-gaap_LongTermDebtNoncurrent_93e33c59-dc8e-46c8-ab2c-a4bfffd73a52" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_4d0fc300-8426-41fc-8393-9a290b5a5d47" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_af567f6d-7da3-412c-afc9-4f99e1cc54b8" xlink:to="loc_us-gaap_DebtInstrumentTable_4d0fc300-8426-41fc-8393-9a290b5a5d47" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_6592eb44-8eb6-4819-ace3-948d18aa1efc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_4d0fc300-8426-41fc-8393-9a290b5a5d47" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_6592eb44-8eb6-4819-ace3-948d18aa1efc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_6592eb44-8eb6-4819-ace3-948d18aa1efc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_6592eb44-8eb6-4819-ace3-948d18aa1efc" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_6592eb44-8eb6-4819-ace3-948d18aa1efc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_4df97459-e265-4d30-9b84-4fc97d7f0f01" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_6592eb44-8eb6-4819-ace3-948d18aa1efc" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_4df97459-e265-4d30-9b84-4fc97d7f0f01" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_941088fa-8e1c-4308-a383-18c6e09f068a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_4df97459-e265-4d30-9b84-4fc97d7f0f01" xlink:to="loc_us-gaap_SecuredDebtMember_941088fa-8e1c-4308-a383-18c6e09f068a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_51c82f8e-c620-4fd1-b98b-39f2487e6130" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_4d0fc300-8426-41fc-8393-9a290b5a5d47" xlink:to="loc_us-gaap_DebtInstrumentAxis_51c82f8e-c620-4fd1-b98b-39f2487e6130" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_51c82f8e-c620-4fd1-b98b-39f2487e6130_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_51c82f8e-c620-4fd1-b98b-39f2487e6130" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_51c82f8e-c620-4fd1-b98b-39f2487e6130_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_e0f85d39-86eb-491a-9aaa-cfc542fce9d6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_51c82f8e-c620-4fd1-b98b-39f2487e6130" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_e0f85d39-86eb-491a-9aaa-cfc542fce9d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_TermLoanAgreementMember_1b60902f-66ef-436e-94a7-948e9f967bd3" xlink:href="asan-20220131.xsd#asan_TermLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_e0f85d39-86eb-491a-9aaa-cfc542fce9d6" xlink:to="loc_asan_TermLoanAgreementMember_1b60902f-66ef-436e-94a7-948e9f967bd3" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/CommitmentsandContingenciesDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#CommitmentsandContingenciesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/CommitmentsandContingenciesDetails" xlink:type="extended" id="i91ca6439c6c84068a13282099f4d01b6_CommitmentsandContingenciesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentLineItems_b071d8f6-ea6a-441a-bc38-909cb4fc57cf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PurchaseCommitmentExcludingLongtermCommitmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LettersOfCreditOutstandingAmount_4a8167a6-011b-4f98-b55c-7ed0bc49a6d4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LettersOfCreditOutstandingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentLineItems_b071d8f6-ea6a-441a-bc38-909cb4fc57cf" xlink:to="loc_us-gaap_LettersOfCreditOutstandingAmount_4a8167a6-011b-4f98-b55c-7ed0bc49a6d4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermPurchaseCommitmentPeriod_4270b2f8-9bc0-4c0a-aefd-8496fc0a6119" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermPurchaseCommitmentPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentLineItems_b071d8f6-ea6a-441a-bc38-909cb4fc57cf" xlink:to="loc_us-gaap_LongtermPurchaseCommitmentPeriod_4270b2f8-9bc0-4c0a-aefd-8496fc0a6119" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseObligation_09913255-8c9f-4e52-b01f-af6bf43aa680" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PurchaseObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentLineItems_b071d8f6-ea6a-441a-bc38-909cb4fc57cf" xlink:to="loc_us-gaap_PurchaseObligation_09913255-8c9f-4e52-b01f-af6bf43aa680" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_PurchaseObligationMaximumOffsettingAmount_f35720b1-da8e-4896-8815-d8b89c7569a0" xlink:href="asan-20220131.xsd#asan_PurchaseObligationMaximumOffsettingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentLineItems_b071d8f6-ea6a-441a-bc38-909cb4fc57cf" xlink:to="loc_asan_PurchaseObligationMaximumOffsettingAmount_f35720b1-da8e-4896-8815-d8b89c7569a0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_PurchaseCommitmentsCreditsEarned_726290ed-b8c9-4768-bbd0-81e67174d9d1" xlink:href="asan-20220131.xsd#asan_PurchaseCommitmentsCreditsEarned"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentLineItems_b071d8f6-ea6a-441a-bc38-909cb4fc57cf" xlink:to="loc_asan_PurchaseCommitmentsCreditsEarned_726290ed-b8c9-4768-bbd0-81e67174d9d1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseCommitmentRemainingMinimumAmountCommitted_e7396745-d80b-432b-a6de-cc816b4f7494" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PurchaseCommitmentRemainingMinimumAmountCommitted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentLineItems_b071d8f6-ea6a-441a-bc38-909cb4fc57cf" xlink:to="loc_us-gaap_PurchaseCommitmentRemainingMinimumAmountCommitted_e7396745-d80b-432b-a6de-cc816b4f7494" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentTable_baf3b59a-c7a6-4951-ac53-4e5696056e29" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PurchaseCommitmentExcludingLongtermCommitmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentLineItems_b071d8f6-ea6a-441a-bc38-909cb4fc57cf" xlink:to="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentTable_baf3b59a-c7a6-4951-ac53-4e5696056e29" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentAxis_7d8f2166-90ee-4af2-9a5d-14b75c3dd8c0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PurchaseCommitmentExcludingLongtermCommitmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentTable_baf3b59a-c7a6-4951-ac53-4e5696056e29" xlink:to="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentAxis_7d8f2166-90ee-4af2-9a5d-14b75c3dd8c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentDomain_7d8f2166-90ee-4af2-9a5d-14b75c3dd8c0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PurchaseCommitmentExcludingLongtermCommitmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentAxis_7d8f2166-90ee-4af2-9a5d-14b75c3dd8c0" xlink:to="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentDomain_7d8f2166-90ee-4af2-9a5d-14b75c3dd8c0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentDomain_ebb68c1f-1987-4a68-a62e-b843771927dc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PurchaseCommitmentExcludingLongtermCommitmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentAxis_7d8f2166-90ee-4af2-9a5d-14b75c3dd8c0" xlink:to="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentDomain_ebb68c1f-1987-4a68-a62e-b843771927dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_HostingRelatedServicesMember_56150818-0ae4-47b8-8733-dd44d85052b5" xlink:href="asan-20220131.xsd#asan_HostingRelatedServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentDomain_ebb68c1f-1987-4a68-a62e-b843771927dc" xlink:to="loc_asan_HostingRelatedServicesMember_56150818-0ae4-47b8-8733-dd44d85052b5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SoftwareBasedServicesMember_4acb2ec1-6626-4947-a547-7f99562127ff" xlink:href="asan-20220131.xsd#asan_SoftwareBasedServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PurchaseCommitmentExcludingLongtermCommitmentDomain_ebb68c1f-1987-4a68-a62e-b843771927dc" xlink:to="loc_asan_SoftwareBasedServicesMember_4acb2ec1-6626-4947-a547-7f99562127ff" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/LeasesNarrativeDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#LeasesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/LeasesNarrativeDetails" xlink:type="extended" id="i744111102ba14ffb8780bcfe575a6c05_LeasesNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_78d28da3-19d5-46c5-b7f3-035a23637aa2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LettersOfCreditOutstandingAmount_b4a46fa2-8603-457a-9f25-5729bb9a7d66" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LettersOfCreditOutstandingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_78d28da3-19d5-46c5-b7f3-035a23637aa2" xlink:to="loc_us-gaap_LettersOfCreditOutstandingAmount_b4a46fa2-8603-457a-9f25-5729bb9a7d66" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_db187c7e-0dac-46f6-a641-42789ab51cf3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_78d28da3-19d5-46c5-b7f3-035a23637aa2" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_db187c7e-0dac-46f6-a641-42789ab51cf3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_22b30e57-36ce-47df-933c-930f78e3132d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_78d28da3-19d5-46c5-b7f3-035a23637aa2" xlink:to="loc_us-gaap_OperatingLeaseLiability_22b30e57-36ce-47df-933c-930f78e3132d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_LesseeOperatingLeaseFutureMinimumPaymentsNetOfTenantImprovementsReceivable_6cc7436d-4a47-47a8-b4ec-6512360f53b8" xlink:href="asan-20220131.xsd#asan_LesseeOperatingLeaseFutureMinimumPaymentsNetOfTenantImprovementsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_78d28da3-19d5-46c5-b7f3-035a23637aa2" xlink:to="loc_asan_LesseeOperatingLeaseFutureMinimumPaymentsNetOfTenantImprovementsReceivable_6cc7436d-4a47-47a8-b4ec-6512360f53b8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_LesseeOperatingLeaseLiabilityUndiscountedAmountNet_1856ed68-bdd9-4ff4-a2b8-005cc74d8f5c" xlink:href="asan-20220131.xsd#asan_LesseeOperatingLeaseLiabilityUndiscountedAmountNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_78d28da3-19d5-46c5-b7f3-035a23637aa2" xlink:to="loc_asan_LesseeOperatingLeaseLiabilityUndiscountedAmountNet_1856ed68-bdd9-4ff4-a2b8-005cc74d8f5c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_LesseeOperatingLeaseFeesUndiscountedAmount_67c44681-73c9-497c-95d7-e46f7d08e0aa" xlink:href="asan-20220131.xsd#asan_LesseeOperatingLeaseFeesUndiscountedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_78d28da3-19d5-46c5-b7f3-035a23637aa2" xlink:to="loc_asan_LesseeOperatingLeaseFeesUndiscountedAmount_67c44681-73c9-497c-95d7-e46f7d08e0aa" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_a5c2f69a-9423-4115-b998-fc38074596f7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_78d28da3-19d5-46c5-b7f3-035a23637aa2" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_a5c2f69a-9423-4115-b998-fc38074596f7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_cb7faaa0-7bd4-4df9-895c-d10371c98ff1" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_a5c2f69a-9423-4115-b998-fc38074596f7" xlink:to="loc_srt_StatementGeographicalAxis_cb7faaa0-7bd4-4df9-895c-d10371c98ff1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_cb7faaa0-7bd4-4df9-895c-d10371c98ff1_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_cb7faaa0-7bd4-4df9-895c-d10371c98ff1" xlink:to="loc_srt_SegmentGeographicalDomain_cb7faaa0-7bd4-4df9-895c-d10371c98ff1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_573835cd-cecf-4147-91e0-d32223432950" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_cb7faaa0-7bd4-4df9-895c-d10371c98ff1" xlink:to="loc_srt_SegmentGeographicalDomain_573835cd-cecf-4147-91e0-d32223432950" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SanFranciscoMember_0b44a884-3923-4527-9c95-45419c6dd689" xlink:href="asan-20220131.xsd#asan_SanFranciscoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_573835cd-cecf-4147-91e0-d32223432950" xlink:to="loc_asan_SanFranciscoMember_0b44a884-3923-4527-9c95-45419c6dd689" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/NetLossperShareAntidilutiveSecuritiesDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#NetLossperShareAntidilutiveSecuritiesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/NetLossperShareAntidilutiveSecuritiesDetails" xlink:type="extended" id="i263f9fb28863467ab6a145889aefd873_NetLossperShareAntidilutiveSecuritiesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasicLineItems_8de41cfa-ccc8-4899-93c2-a73d96f9123e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EarningsPerShareBasicLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_1141ec08-8c30-486a-b3b0-237bb8e3c763" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareBasicLineItems_8de41cfa-ccc8-4899-93c2-a73d96f9123e" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_1141ec08-8c30-486a-b3b0-237bb8e3c763" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_f194fbe7-7015-43db-a76e-b196ea218daa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareBasicLineItems_8de41cfa-ccc8-4899-93c2-a73d96f9123e" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_f194fbe7-7015-43db-a76e-b196ea218daa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEarningsPerShareBasicByCommonClassTable_5e464bf6-c104-4b12-8558-17495d351002" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfEarningsPerShareBasicByCommonClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_EarningsPerShareBasicLineItems_8de41cfa-ccc8-4899-93c2-a73d96f9123e" xlink:to="loc_us-gaap_ScheduleOfEarningsPerShareBasicByCommonClassTable_5e464bf6-c104-4b12-8558-17495d351002" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_9b662710-483b-49f5-9a90-ca94c51862e9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEarningsPerShareBasicByCommonClassTable_5e464bf6-c104-4b12-8558-17495d351002" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_9b662710-483b-49f5-9a90-ca94c51862e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_9b662710-483b-49f5-9a90-ca94c51862e9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_9b662710-483b-49f5-9a90-ca94c51862e9" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_9b662710-483b-49f5-9a90-ca94c51862e9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a61f31b0-0652-4306-9eb0-2dad802cffd4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_9b662710-483b-49f5-9a90-ca94c51862e9" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a61f31b0-0652-4306-9eb0-2dad802cffd4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RedeemableConvertiblePreferredStockMember_82a8e3ca-972d-4ef6-afb8-1586b2dbf793" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RedeemableConvertiblePreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a61f31b0-0652-4306-9eb0-2dad802cffd4" xlink:to="loc_us-gaap_RedeemableConvertiblePreferredStockMember_82a8e3ca-972d-4ef6-afb8-1586b2dbf793" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_3d9e79f5-8876-4f9b-891a-1c2683869bcc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a61f31b0-0652-4306-9eb0-2dad802cffd4" xlink:to="loc_us-gaap_EmployeeStockOptionMember_3d9e79f5-8876-4f9b-891a-1c2683869bcc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_2ca9bc9d-63fe-4688-ba97-a32ddb7a5c53" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a61f31b0-0652-4306-9eb0-2dad802cffd4" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_2ca9bc9d-63fe-4688-ba97-a32ddb7a5c53" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_EarlyExercisedStockOptionsMember_e038f26e-c708-4f98-b57e-6df3b7dc1bc1" xlink:href="asan-20220131.xsd#asan_EarlyExercisedStockOptionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a61f31b0-0652-4306-9eb0-2dad802cffd4" xlink:to="loc_asan_EarlyExercisedStockOptionsMember_e038f26e-c708-4f98-b57e-6df3b7dc1bc1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_cb8f2782-9b43-489b-aa1c-6aecf42e91cc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a61f31b0-0652-4306-9eb0-2dad802cffd4" xlink:to="loc_us-gaap_EmployeeStockMember_cb8f2782-9b43-489b-aa1c-6aecf42e91cc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_d8d3ae17-2c46-45f8-b5db-7b2af6ea80cb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a61f31b0-0652-4306-9eb0-2dad802cffd4" xlink:to="loc_us-gaap_CommonClassBMember_d8d3ae17-2c46-45f8-b5db-7b2af6ea80cb" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_14f497b9-7ad1-49df-8995-3d3f56c47088" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEarningsPerShareBasicByCommonClassTable_5e464bf6-c104-4b12-8558-17495d351002" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_14f497b9-7ad1-49df-8995-3d3f56c47088" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_14f497b9-7ad1-49df-8995-3d3f56c47088_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_14f497b9-7ad1-49df-8995-3d3f56c47088" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_14f497b9-7ad1-49df-8995-3d3f56c47088_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_aa64bd7b-0b45-46ed-8931-52e543a225a9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_14f497b9-7ad1-49df-8995-3d3f56c47088" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_aa64bd7b-0b45-46ed-8931-52e543a225a9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtMember_130c7964-d4d5-403e-bbce-81ba7f3bf4c1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConvertibleDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_aa64bd7b-0b45-46ed-8931-52e543a225a9" xlink:to="loc_us-gaap_ConvertibleDebtMember_130c7964-d4d5-403e-bbce-81ba7f3bf4c1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_9ba5bc73-060e-4007-9f9f-65c9e9eb95d8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEarningsPerShareBasicByCommonClassTable_5e464bf6-c104-4b12-8558-17495d351002" xlink:to="loc_us-gaap_StatementClassOfStockAxis_9ba5bc73-060e-4007-9f9f-65c9e9eb95d8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_9ba5bc73-060e-4007-9f9f-65c9e9eb95d8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_9ba5bc73-060e-4007-9f9f-65c9e9eb95d8" xlink:to="loc_us-gaap_ClassOfStockDomain_9ba5bc73-060e-4007-9f9f-65c9e9eb95d8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_de025c5f-6c8b-4c5c-827f-85a25f05b655" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_9ba5bc73-060e-4007-9f9f-65c9e9eb95d8" xlink:to="loc_us-gaap_ClassOfStockDomain_de025c5f-6c8b-4c5c-827f-85a25f05b655" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_dcb8eb70-1461-4e4d-982e-132a16878c0c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_de025c5f-6c8b-4c5c-827f-85a25f05b655" xlink:to="loc_us-gaap_CommonClassBMember_dcb8eb70-1461-4e4d-982e-132a16878c0c" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersEquityDeficitNarrativeDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#StockholdersEquityDeficitNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersEquityDeficitNarrativeDetails" xlink:type="extended" id="ib6aeb233076d435985f22f18a4f4dd1c_StockholdersEquityDeficitNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_22f7f24f-e60f-4fa6-ba75-7ba409a7b886" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_22f7f24f-e60f-4fa6-ba75-7ba409a7b886" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_NumberOfVotesPerShare_b54d397d-3536-4c91-8796-73743f7148d0" xlink:href="asan-20220131.xsd#asan_NumberOfVotesPerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_asan_NumberOfVotesPerShare_b54d397d-3536-4c91-8796-73743f7148d0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConversionOfStockSharesConverted1_c15f13bf-5b25-4179-9fdb-f805d22d33ef" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConversionOfStockSharesConverted1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_ConversionOfStockSharesConverted1_c15f13bf-5b25-4179-9fdb-f805d22d33ef" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_a817119f-2bd2-4b43-a3d6-4e1ab3bd361c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_CommonStockSharesIssued_a817119f-2bd2-4b43-a3d6-4e1ab3bd361c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_b3f38227-4b95-4bba-99f0-e5935876d336" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_b3f38227-4b95-4bba-99f0-e5935876d336" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_e37f3ee8-4959-4418-a664-0bb96400e653" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_e37f3ee8-4959-4418-a664-0bb96400e653" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardMinimumExercisePricePercentOfFairValueGrantDatePrice_e5444812-0304-4b05-903f-facab7fd39c0" xlink:href="asan-20220131.xsd#asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardMinimumExercisePricePercentOfFairValueGrantDatePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardMinimumExercisePricePercentOfFairValueGrantDatePrice_e5444812-0304-4b05-903f-facab7fd39c0" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_b25d4260-7493-4825-9e09-82e1a1943452" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_b25d4260-7493-4825-9e09-82e1a1943452" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage_c2d3ed4f-c62a-43c5-b9e4-b0d552184295" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage_c2d3ed4f-c62a-43c5-b9e4-b0d552184295" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_fd0b6760-1625-4028-87ff-cfb51e3e3f01" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_fd0b6760-1625-4028-87ff-cfb51e3e3f01" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodShares_c71a9886-89da-4ef2-85b0-fa3ecaacf3d2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockRepurchasedDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodShares_c71a9886-89da-4ef2-85b0-fa3ecaacf3d2" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharePrice_a0d5c256-7dc0-4b6e-a2da-5574c1636fbe" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_SharePrice_a0d5c256-7dc0-4b6e-a2da-5574c1636fbe" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_c0d8274e-60f5-443c-bfcd-d2d6ff385651" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsForRepurchaseOfCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_c0d8274e-60f5-443c-bfcd-d2d6ff385651" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeBenefitsAndShareBasedCompensation_1fecf7aa-9394-459e-9f23-59693617dab6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeBenefitsAndShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_EmployeeBenefitsAndShareBasedCompensation_1fecf7aa-9394-459e-9f23-59693617dab6" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_0b8311f1-0ef0-4985-9b8b-2a163c20cbf4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_0b8311f1-0ef0-4985-9b8b-2a163c20cbf4" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardIncreaseInNumberOfSharesAvailableForGrant_08297b3e-6107-4f9d-8d0c-41e0e54bc485" xlink:href="asan-20220131.xsd#asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardIncreaseInNumberOfSharesAvailableForGrant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardIncreaseInNumberOfSharesAvailableForGrant_08297b3e-6107-4f9d-8d0c-41e0e54bc485" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_fc91e283-f13f-4a45-a5af-571cfe646e9b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_fc91e283-f13f-4a45-a5af-571cfe646e9b" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockOwnershipPlan_51dbc464-4363-4d6c-8121-412ff4c0860f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesEmployeeStockOwnershipPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockOwnershipPlan_51dbc464-4363-4d6c-8121-412ff4c0860f" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_518efae6-6b2e-4205-8d58-94d98979e88d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_518efae6-6b2e-4205-8d58-94d98979e88d" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_ShareBasedPaymentArrangementEmployeeContributionsWithheld_88a58e64-ad73-4ade-836a-d96fdbbd2f42" xlink:href="asan-20220131.xsd#asan_ShareBasedPaymentArrangementEmployeeContributionsWithheld"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_asan_ShareBasedPaymentArrangementEmployeeContributionsWithheld_88a58e64-ad73-4ade-836a-d96fdbbd2f42" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_5e4d533c-34fc-41c5-b882-0680973c81ce" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_5e4d533c-34fc-41c5-b882-0680973c81ce" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_d877f079-a887-479d-93a7-6769afd5eb64" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_d877f079-a887-479d-93a7-6769afd5eb64" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_d9506ea2-2228-48d5-a246-8e240032d3f0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c3bd3684-54c1-4f82-95af-8b8fd76eeb7a" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_d9506ea2-2228-48d5-a246-8e240032d3f0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_160d8996-b6e1-4891-80fd-261db35bf9b9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_d9506ea2-2228-48d5-a246-8e240032d3f0" xlink:to="loc_us-gaap_StatementClassOfStockAxis_160d8996-b6e1-4891-80fd-261db35bf9b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_160d8996-b6e1-4891-80fd-261db35bf9b9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_160d8996-b6e1-4891-80fd-261db35bf9b9" xlink:to="loc_us-gaap_ClassOfStockDomain_160d8996-b6e1-4891-80fd-261db35bf9b9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_3d8a266a-c638-4df3-aa13-2dbeedd69bf6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_160d8996-b6e1-4891-80fd-261db35bf9b9" xlink:to="loc_us-gaap_ClassOfStockDomain_3d8a266a-c638-4df3-aa13-2dbeedd69bf6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_3dade595-d749-485d-a3ad-d5711a86ed96" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_3d8a266a-c638-4df3-aa13-2dbeedd69bf6" xlink:to="loc_us-gaap_CommonClassAMember_3dade595-d749-485d-a3ad-d5711a86ed96" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_533ce32c-2db2-4d00-b547-78e1d9e40e38" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_3d8a266a-c638-4df3-aa13-2dbeedd69bf6" xlink:to="loc_us-gaap_CommonClassBMember_533ce32c-2db2-4d00-b547-78e1d9e40e38" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_4250a16a-8913-4cb6-b494-11d122085d00" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_d9506ea2-2228-48d5-a246-8e240032d3f0" xlink:to="loc_us-gaap_AwardTypeAxis_4250a16a-8913-4cb6-b494-11d122085d00" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_4250a16a-8913-4cb6-b494-11d122085d00_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_4250a16a-8913-4cb6-b494-11d122085d00" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_4250a16a-8913-4cb6-b494-11d122085d00_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_17260919-06d3-4f5b-8cef-b16a43459f31" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_4250a16a-8913-4cb6-b494-11d122085d00" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_17260919-06d3-4f5b-8cef-b16a43459f31" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_1ac7ee3b-f01e-463c-a06a-6566fdebf851" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_17260919-06d3-4f5b-8cef-b16a43459f31" xlink:to="loc_us-gaap_EmployeeStockOptionMember_1ac7ee3b-f01e-463c-a06a-6566fdebf851" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_ec894daa-57ed-4d30-93e4-02cf44ce3756" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_17260919-06d3-4f5b-8cef-b16a43459f31" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_ec894daa-57ed-4d30-93e4-02cf44ce3756" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_409ef6ae-9c44-44c7-a8fa-62e803060a2e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_17260919-06d3-4f5b-8cef-b16a43459f31" xlink:to="loc_us-gaap_EmployeeStockMember_409ef6ae-9c44-44c7-a8fa-62e803060a2e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_42013b92-4596-4c6c-8d4f-739124621b47" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_d9506ea2-2228-48d5-a246-8e240032d3f0" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_42013b92-4596-4c6c-8d4f-739124621b47" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_42013b92-4596-4c6c-8d4f-739124621b47_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_42013b92-4596-4c6c-8d4f-739124621b47" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_42013b92-4596-4c6c-8d4f-739124621b47_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_2a54765a-25c0-41eb-8d80-a292e15f1ac5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_42013b92-4596-4c6c-8d4f-739124621b47" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_2a54765a-25c0-41eb-8d80-a292e15f1ac5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_EarlyExercisedStockOptionsMember_2a8934f6-9ddd-4b09-83d1-68991b6a7c57" xlink:href="asan-20220131.xsd#asan_EarlyExercisedStockOptionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_2a54765a-25c0-41eb-8d80-a292e15f1ac5" xlink:to="loc_asan_EarlyExercisedStockOptionsMember_2a8934f6-9ddd-4b09-83d1-68991b6a7c57" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingAxis_94d171e3-b1c5-4c4f-8c3f-8a9ac88bf8d1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VestingAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_d9506ea2-2228-48d5-a246-8e240032d3f0" xlink:to="loc_us-gaap_VestingAxis_94d171e3-b1c5-4c4f-8c3f-8a9ac88bf8d1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingDomain_94d171e3-b1c5-4c4f-8c3f-8a9ac88bf8d1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VestingDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VestingAxis_94d171e3-b1c5-4c4f-8c3f-8a9ac88bf8d1" xlink:to="loc_us-gaap_VestingDomain_94d171e3-b1c5-4c4f-8c3f-8a9ac88bf8d1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingDomain_ca8bcbfb-813d-4167-9848-14360e913de6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VestingDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VestingAxis_94d171e3-b1c5-4c4f-8c3f-8a9ac88bf8d1" xlink:to="loc_us-gaap_VestingDomain_ca8bcbfb-813d-4167-9848-14360e913de6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationAwardTrancheOneMember_905c0529-e301-44cf-b485-d2d674816fe8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationAwardTrancheOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VestingDomain_ca8bcbfb-813d-4167-9848-14360e913de6" xlink:to="loc_us-gaap_ShareBasedCompensationAwardTrancheOneMember_905c0529-e301-44cf-b485-d2d674816fe8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationAwardTrancheTwoMember_7bb1722c-4264-401b-bb1c-491ebdaa9519" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationAwardTrancheTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VestingDomain_ca8bcbfb-813d-4167-9848-14360e913de6" xlink:to="loc_us-gaap_ShareBasedCompensationAwardTrancheTwoMember_7bb1722c-4264-401b-bb1c-491ebdaa9519" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramAxis_f543ba73-f628-4886-9f75-3dd976bc971d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareRepurchaseProgramAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_d9506ea2-2228-48d5-a246-8e240032d3f0" xlink:to="loc_us-gaap_ShareRepurchaseProgramAxis_f543ba73-f628-4886-9f75-3dd976bc971d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramDomain_f543ba73-f628-4886-9f75-3dd976bc971d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareRepurchaseProgramDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ShareRepurchaseProgramAxis_f543ba73-f628-4886-9f75-3dd976bc971d" xlink:to="loc_us-gaap_ShareRepurchaseProgramDomain_f543ba73-f628-4886-9f75-3dd976bc971d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramDomain_42b7c26f-6fc0-42fe-8c26-22a0c6f67336" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareRepurchaseProgramDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ShareRepurchaseProgramAxis_f543ba73-f628-4886-9f75-3dd976bc971d" xlink:to="loc_us-gaap_ShareRepurchaseProgramDomain_42b7c26f-6fc0-42fe-8c26-22a0c6f67336" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_A2020TenderOfferMember_ecac0656-a43d-4829-ab68-f5af43142304" xlink:href="asan-20220131.xsd#asan_A2020TenderOfferMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareRepurchaseProgramDomain_42b7c26f-6fc0-42fe-8c26-22a0c6f67336" xlink:to="loc_asan_A2020TenderOfferMember_ecac0656-a43d-4829-ab68-f5af43142304" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersEquityDeficitFairValueAssumptionsDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#StockholdersEquityDeficitFairValueAssumptionsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersEquityDeficitFairValueAssumptionsDetails" xlink:type="extended" id="i1503be39fe55497b8ea59ef9ef335b45_StockholdersEquityDeficitFairValueAssumptionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_771d30bb-238c-482a-af5e-c0017c48e779" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_fa5acdf1-839c-4d75-a741-7e4ff4c1e439" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_771d30bb-238c-482a-af5e-c0017c48e779" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_fa5acdf1-839c-4d75-a741-7e4ff4c1e439" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_3ae0cba2-eb4b-484b-8052-dafe3a952c6d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_771d30bb-238c-482a-af5e-c0017c48e779" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_3ae0cba2-eb4b-484b-8052-dafe3a952c6d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_e2c55426-2868-4919-bdce-6fe0eab4ccb5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_771d30bb-238c-482a-af5e-c0017c48e779" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_e2c55426-2868-4919-bdce-6fe0eab4ccb5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_ff5fc0f4-3a4c-49bc-ae4c-2c913a5061f9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_771d30bb-238c-482a-af5e-c0017c48e779" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_ff5fc0f4-3a4c-49bc-ae4c-2c913a5061f9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_41e2e656-eebf-4463-8870-69d793b44557" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_771d30bb-238c-482a-af5e-c0017c48e779" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_41e2e656-eebf-4463-8870-69d793b44557" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_f1edd0d0-02cb-4b8d-9fcd-c6e9e2aa850a" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_41e2e656-eebf-4463-8870-69d793b44557" xlink:to="loc_srt_RangeAxis_f1edd0d0-02cb-4b8d-9fcd-c6e9e2aa850a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_f1edd0d0-02cb-4b8d-9fcd-c6e9e2aa850a_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_f1edd0d0-02cb-4b8d-9fcd-c6e9e2aa850a" xlink:to="loc_srt_RangeMember_f1edd0d0-02cb-4b8d-9fcd-c6e9e2aa850a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_fce9f354-043f-482f-be80-28555e6845c9" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_f1edd0d0-02cb-4b8d-9fcd-c6e9e2aa850a" xlink:to="loc_srt_RangeMember_fce9f354-043f-482f-be80-28555e6845c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_46ccb74a-033d-481b-9952-2d580655df48" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_fce9f354-043f-482f-be80-28555e6845c9" xlink:to="loc_srt_MinimumMember_46ccb74a-033d-481b-9952-2d580655df48" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_6e586b76-864d-4d2c-b95e-a5f768068ddd" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_fce9f354-043f-482f-be80-28555e6845c9" xlink:to="loc_srt_MaximumMember_6e586b76-864d-4d2c-b95e-a5f768068ddd" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersEquityDeficitScheduleofRSUActivityDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#StockholdersEquityDeficitScheduleofRSUActivityDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersEquityDeficitScheduleofRSUActivityDetails" xlink:type="extended" id="i7a16628fd9b9424c9f833c47e579b3a1_StockholdersEquityDeficitScheduleofRSUActivityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_dac46457-a380-455b-b805-0403c75e4dc8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_47c33ee6-87a5-4a69-8b36-5ce5ec03e4fd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_dac46457-a380-455b-b805-0403c75e4dc8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_47c33ee6-87a5-4a69-8b36-5ce5ec03e4fd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_0cd649ae-6a91-4ec9-bd39-f27d07b1f314" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_47c33ee6-87a5-4a69-8b36-5ce5ec03e4fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_0cd649ae-6a91-4ec9-bd39-f27d07b1f314" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_0310f2eb-dbaf-4a90-8f22-d2b5f44d1e2d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_47c33ee6-87a5-4a69-8b36-5ce5ec03e4fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_0310f2eb-dbaf-4a90-8f22-d2b5f44d1e2d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_a3f525dc-48c0-4d39-a9f9-4eb80086ebab" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_47c33ee6-87a5-4a69-8b36-5ce5ec03e4fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_a3f525dc-48c0-4d39-a9f9-4eb80086ebab" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_a0da22c8-74f9-4757-ae5f-37854082cecf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_47c33ee6-87a5-4a69-8b36-5ce5ec03e4fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_a0da22c8-74f9-4757-ae5f-37854082cecf" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_dad96643-77d7-4945-a314-53084825b24c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotReleased_f6c387c2-6b02-491c-a32d-8332156d6b86" xlink:href="asan-20220131.xsd#asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotReleased"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_dac46457-a380-455b-b805-0403c75e4dc8" xlink:to="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotReleased_f6c387c2-6b02-491c-a32d-8332156d6b86" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_f9ca8955-4ac2-495d-85b3-f3c4976d8eee" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_dac46457-a380-455b-b805-0403c75e4dc8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_f9ca8955-4ac2-495d-85b3-f3c4976d8eee" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_3b3eebbf-f6b5-43a7-ac74-3c488ff6e6cf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_f9ca8955-4ac2-495d-85b3-f3c4976d8eee" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_3b3eebbf-f6b5-43a7-ac74-3c488ff6e6cf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_1865fd43-4204-4f7c-b302-a0b96ca47c1a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_f9ca8955-4ac2-495d-85b3-f3c4976d8eee" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_1865fd43-4204-4f7c-b302-a0b96ca47c1a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_0a27aa1d-9e4b-4a33-867d-6496055dcdde" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_f9ca8955-4ac2-495d-85b3-f3c4976d8eee" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_0a27aa1d-9e4b-4a33-867d-6496055dcdde" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_d152d57a-bf12-4ed6-a76f-81304883fdd7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_f9ca8955-4ac2-495d-85b3-f3c4976d8eee" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_d152d57a-bf12-4ed6-a76f-81304883fdd7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_a7f3137f-03db-48ea-a5db-89f417503cfd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotSettledWeightedAverageGrantDateFairValue_246ba50c-39e7-4461-9cfa-bc49832f7f77" xlink:href="asan-20220131.xsd#asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotSettledWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_dac46457-a380-455b-b805-0403c75e4dc8" xlink:to="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotSettledWeightedAverageGrantDateFairValue_246ba50c-39e7-4461-9cfa-bc49832f7f77" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardEquityInstrumentsOtherThanOptionsAggregateIntrinsicValueOutstanding_16f60cbf-04a1-4960-acf5-cfa3d6862358" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardEquityInstrumentsOtherThanOptionsAggregateIntrinsicValueOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_dac46457-a380-455b-b805-0403c75e4dc8" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardEquityInstrumentsOtherThanOptionsAggregateIntrinsicValueOutstanding_16f60cbf-04a1-4960-acf5-cfa3d6862358" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_9de994af-a211-4468-8def-8407718d0acc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_dac46457-a380-455b-b805-0403c75e4dc8" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_9de994af-a211-4468-8def-8407718d0acc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_dd2891ef-a7cd-4691-bd26-1748cbfd587a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_9de994af-a211-4468-8def-8407718d0acc" xlink:to="loc_us-gaap_AwardTypeAxis_dd2891ef-a7cd-4691-bd26-1748cbfd587a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_dd2891ef-a7cd-4691-bd26-1748cbfd587a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_dd2891ef-a7cd-4691-bd26-1748cbfd587a" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_dd2891ef-a7cd-4691-bd26-1748cbfd587a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_30d956ed-90f3-4c37-b908-fb94a62e4976" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_dd2891ef-a7cd-4691-bd26-1748cbfd587a" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_30d956ed-90f3-4c37-b908-fb94a62e4976" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_a36fa97b-a7e2-492f-b01f-6784990427dd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_30d956ed-90f3-4c37-b908-fb94a62e4976" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_a36fa97b-a7e2-492f-b01f-6784990427dd" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersEquityDeficitStockbasedCompensationExpenseDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#StockholdersEquityDeficitStockbasedCompensationExpenseDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersEquityDeficitStockbasedCompensationExpenseDetails" xlink:type="extended" id="i37a9e64ce2d64b62bad76562e4c92e61_StockholdersEquityDeficitStockbasedCompensationExpenseDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_053af693-984b-4daa-b995-9051090bd927" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_a63a3f6d-d1c9-4d4d-9be0-5e5ced1ccc1e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_053af693-984b-4daa-b995-9051090bd927" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_a63a3f6d-d1c9-4d4d-9be0-5e5ced1ccc1e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_e985a18d-2571-4e46-9f5e-bd7affeb7f64" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_053af693-984b-4daa-b995-9051090bd927" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_e985a18d-2571-4e46-9f5e-bd7affeb7f64" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_2b0446ca-3962-4cea-be4d-0c664b7ed8e1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_053af693-984b-4daa-b995-9051090bd927" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_2b0446ca-3962-4cea-be4d-0c664b7ed8e1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_592c3c81-2532-471d-88c2-dcd099f1392a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_053af693-984b-4daa-b995-9051090bd927" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_592c3c81-2532-471d-88c2-dcd099f1392a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms_3a3b97f3-3702-453a-a7cd-95183c99b5cc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_053af693-984b-4daa-b995-9051090bd927" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms_3a3b97f3-3702-453a-a7cd-95183c99b5cc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_6f217dbb-9341-41df-939b-7666df7ae8bf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_053af693-984b-4daa-b995-9051090bd927" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_6f217dbb-9341-41df-939b-7666df7ae8bf" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_42f14fb4-a5ec-4a05-95f3-fe1ee0fe7cfa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_6f217dbb-9341-41df-939b-7666df7ae8bf" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_42f14fb4-a5ec-4a05-95f3-fe1ee0fe7cfa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_42f14fb4-a5ec-4a05-95f3-fe1ee0fe7cfa_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_42f14fb4-a5ec-4a05-95f3-fe1ee0fe7cfa" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_42f14fb4-a5ec-4a05-95f3-fe1ee0fe7cfa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_96fea5b2-cd97-4dcb-96b9-c370b99eb849" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_42f14fb4-a5ec-4a05-95f3-fe1ee0fe7cfa" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_96fea5b2-cd97-4dcb-96b9-c370b99eb849" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_ec60ce9a-3f1c-4f2a-ba67-027209ab6cda" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_96fea5b2-cd97-4dcb-96b9-c370b99eb849" xlink:to="loc_us-gaap_CostOfSalesMember_ec60ce9a-3f1c-4f2a-ba67-027209ab6cda" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_3e4bd8ee-00ae-48d8-863c-77cec81ae1da" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_96fea5b2-cd97-4dcb-96b9-c370b99eb849" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_3e4bd8ee-00ae-48d8-863c-77cec81ae1da" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpenseMember_cb58a74c-3958-44f0-bffe-0a8d66422e0a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SellingAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_96fea5b2-cd97-4dcb-96b9-c370b99eb849" xlink:to="loc_us-gaap_SellingAndMarketingExpenseMember_cb58a74c-3958-44f0-bffe-0a8d66422e0a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_2d72bd8b-a98c-4e54-b993-b886b983cfe8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_96fea5b2-cd97-4dcb-96b9-c370b99eb849" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_2d72bd8b-a98c-4e54-b993-b886b983cfe8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_bc848ee6-fcbc-4d38-902d-4c10679b45b4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_6f217dbb-9341-41df-939b-7666df7ae8bf" xlink:to="loc_us-gaap_AwardTypeAxis_bc848ee6-fcbc-4d38-902d-4c10679b45b4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_bc848ee6-fcbc-4d38-902d-4c10679b45b4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_bc848ee6-fcbc-4d38-902d-4c10679b45b4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_bc848ee6-fcbc-4d38-902d-4c10679b45b4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_36804489-e18c-4f5a-94c6-a4fa65309262" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_bc848ee6-fcbc-4d38-902d-4c10679b45b4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_36804489-e18c-4f5a-94c6-a4fa65309262" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_1ef86f5a-9579-40eb-bcc6-2050ea8d8a25" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_36804489-e18c-4f5a-94c6-a4fa65309262" xlink:to="loc_us-gaap_EmployeeStockOptionMember_1ef86f5a-9579-40eb-bcc6-2050ea8d8a25" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_17189179-1085-483f-bfaa-628cc6e0cbc1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_36804489-e18c-4f5a-94c6-a4fa65309262" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_17189179-1085-483f-bfaa-628cc6e0cbc1" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersEquityDeficitESPPFairValueAssumptionsDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#StockholdersEquityDeficitESPPFairValueAssumptionsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersEquityDeficitESPPFairValueAssumptionsDetails" xlink:type="extended" id="ied6fbae509ff4a6f9d95efeb95f3abc7_StockholdersEquityDeficitESPPFairValueAssumptionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6d62ac3e-5d09-49a9-976e-9b803e732513" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_16a9ecf4-ecf3-464b-be91-ede154eab9b9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6d62ac3e-5d09-49a9-976e-9b803e732513" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_16a9ecf4-ecf3-464b-be91-ede154eab9b9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_de4e06d3-ec8e-4ab4-83d7-ec53267f8c10" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6d62ac3e-5d09-49a9-976e-9b803e732513" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_de4e06d3-ec8e-4ab4-83d7-ec53267f8c10" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_142a1957-163f-4236-a3f9-b65b6cae6940" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6d62ac3e-5d09-49a9-976e-9b803e732513" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_142a1957-163f-4236-a3f9-b65b6cae6940" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_75a73263-6a8a-49d9-9700-bb000f3fdfa0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6d62ac3e-5d09-49a9-976e-9b803e732513" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_75a73263-6a8a-49d9-9700-bb000f3fdfa0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_08753a81-ff1c-4fe7-acfb-796e0db8eee4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6d62ac3e-5d09-49a9-976e-9b803e732513" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_08753a81-ff1c-4fe7-acfb-796e0db8eee4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_1a4c604b-8b97-4c1b-8554-d0765dd42c75" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_08753a81-ff1c-4fe7-acfb-796e0db8eee4" xlink:to="loc_us-gaap_AwardTypeAxis_1a4c604b-8b97-4c1b-8554-d0765dd42c75" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1a4c604b-8b97-4c1b-8554-d0765dd42c75_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_1a4c604b-8b97-4c1b-8554-d0765dd42c75" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1a4c604b-8b97-4c1b-8554-d0765dd42c75_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_b0a7bdef-75d5-4cd5-8099-7a54b0d06997" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_1a4c604b-8b97-4c1b-8554-d0765dd42c75" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_b0a7bdef-75d5-4cd5-8099-7a54b0d06997" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_27f48fe6-0a79-4bbd-8b89-a3b6b8dfeaba" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_b0a7bdef-75d5-4cd5-8099-7a54b0d06997" xlink:to="loc_us-gaap_EmployeeStockMember_27f48fe6-0a79-4bbd-8b89-a3b6b8dfeaba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_5c58cc34-b6ca-4830-9fbf-49ec01a2f379" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_08753a81-ff1c-4fe7-acfb-796e0db8eee4" xlink:to="loc_srt_RangeAxis_5c58cc34-b6ca-4830-9fbf-49ec01a2f379" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_5c58cc34-b6ca-4830-9fbf-49ec01a2f379_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_5c58cc34-b6ca-4830-9fbf-49ec01a2f379" xlink:to="loc_srt_RangeMember_5c58cc34-b6ca-4830-9fbf-49ec01a2f379_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_1996997d-3c29-4990-b9b5-bfe93f1b04ad" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_5c58cc34-b6ca-4830-9fbf-49ec01a2f379" xlink:to="loc_srt_RangeMember_1996997d-3c29-4990-b9b5-bfe93f1b04ad" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_ea99c036-6033-4502-afc5-a59c4356c624" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_1996997d-3c29-4990-b9b5-bfe93f1b04ad" xlink:to="loc_srt_MinimumMember_ea99c036-6033-4502-afc5-a59c4356c624" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_1336aecb-c056-4f8c-a0df-710a31fc73c0" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_1996997d-3c29-4990-b9b5-bfe93f1b04ad" xlink:to="loc_srt_MaximumMember_1336aecb-c056-4f8c-a0df-710a31fc73c0" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/IncomeTaxesNarrativeDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#IncomeTaxesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/IncomeTaxesNarrativeDetails" xlink:type="extended" id="i74f3f920f1f24500beb38ccd40a90ae0_IncomeTaxesNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxContingencyLineItems_a23a8475-1d75-4ab4-a59a-4a190621327c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxContingencyLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowanceDeferredTaxAssetChangeInAmount_f234fef4-6e8b-47ff-a2ab-376ab85ca64e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ValuationAllowanceDeferredTaxAssetChangeInAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxContingencyLineItems_a23a8475-1d75-4ab4-a59a-4a190621327c" xlink:to="loc_us-gaap_ValuationAllowanceDeferredTaxAssetChangeInAmount_f234fef4-6e8b-47ff-a2ab-376ab85ca64e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwards_190ec74d-e38d-4532-a8a9-5e1f12d691d6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLossCarryforwards"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxContingencyLineItems_a23a8475-1d75-4ab4-a59a-4a190621327c" xlink:to="loc_us-gaap_OperatingLossCarryforwards_190ec74d-e38d-4532-a8a9-5e1f12d691d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch_ae413aa0-ac2c-418c-8805-38f8b4c65d7e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxContingencyLineItems_a23a8475-1d75-4ab4-a59a-4a190621327c" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch_ae413aa0-ac2c-418c-8805-38f8b4c65d7e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecognizedTaxBenefitsIncomeTaxPenaltiesAndInterestAccrued_800c7965-e123-4d5e-a6ab-d2c62ab61d73" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_UnrecognizedTaxBenefitsIncomeTaxPenaltiesAndInterestAccrued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxContingencyLineItems_a23a8475-1d75-4ab4-a59a-4a190621327c" xlink:to="loc_us-gaap_UnrecognizedTaxBenefitsIncomeTaxPenaltiesAndInterestAccrued_800c7965-e123-4d5e-a6ab-d2c62ab61d73" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecognizedTaxBenefits_03d02771-cc81-418e-a5ac-0575781565b7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_UnrecognizedTaxBenefits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxContingencyLineItems_a23a8475-1d75-4ab4-a59a-4a190621327c" xlink:to="loc_us-gaap_UnrecognizedTaxBenefits_03d02771-cc81-418e-a5ac-0575781565b7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxContingencyTable_8d314db6-6915-4715-8efa-01260d7423de" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxContingencyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_IncomeTaxContingencyLineItems_a23a8475-1d75-4ab4-a59a-4a190621327c" xlink:to="loc_us-gaap_IncomeTaxContingencyTable_8d314db6-6915-4715-8efa-01260d7423de" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityAxis_25b654d8-e0df-410f-af18-59af1b7adfa5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxAuthorityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_IncomeTaxContingencyTable_8d314db6-6915-4715-8efa-01260d7423de" xlink:to="loc_us-gaap_IncomeTaxAuthorityAxis_25b654d8-e0df-410f-af18-59af1b7adfa5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_25b654d8-e0df-410f-af18-59af1b7adfa5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_25b654d8-e0df-410f-af18-59af1b7adfa5" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_25b654d8-e0df-410f-af18-59af1b7adfa5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_dcadc11b-de3c-41af-9424-c36005240448" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_25b654d8-e0df-410f-af18-59af1b7adfa5" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_dcadc11b-de3c-41af-9424-c36005240448" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticCountryMember_1c3031ea-54e4-4b86-9853-1914fe5b7807" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DomesticCountryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_dcadc11b-de3c-41af-9424-c36005240448" xlink:to="loc_us-gaap_DomesticCountryMember_1c3031ea-54e4-4b86-9853-1914fe5b7807" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StateAndLocalJurisdictionMember_c5d2164d-638f-406a-9bfb-a5870a79d384" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StateAndLocalJurisdictionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_dcadc11b-de3c-41af-9424-c36005240448" xlink:to="loc_us-gaap_StateAndLocalJurisdictionMember_c5d2164d-638f-406a-9bfb-a5870a79d384" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/GeographicInformationDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#GeographicInformationDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/GeographicInformationDetails" xlink:type="extended" id="icbf836e35176439ba3a8d953f3407e38_GeographicInformationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_d0b19f46-43dc-45a7-be59-24710ceed65b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SegmentReportingRevenueReconcilingItemLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_abcf36a5-ff5a-4425-9a69-486ea31886d7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_d0b19f46-43dc-45a7-be59-24710ceed65b" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_abcf36a5-ff5a-4425-9a69-486ea31886d7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoncurrentAssets_39b5e0ff-348f-4aa2-aef2-6991d6db9481" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NoncurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_d0b19f46-43dc-45a7-be59-24710ceed65b" xlink:to="loc_us-gaap_NoncurrentAssets_39b5e0ff-348f-4aa2-aef2-6991d6db9481" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_4f1c0fb2-9cdb-42eb-bfc2-15d45a9e3d2f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_d0b19f46-43dc-45a7-be59-24710ceed65b" xlink:to="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_4f1c0fb2-9cdb-42eb-bfc2-15d45a9e3d2f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_d0a9f2af-ca3c-48ca-b567-7e7dca26beaa" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_4f1c0fb2-9cdb-42eb-bfc2-15d45a9e3d2f" xlink:to="loc_srt_StatementGeographicalAxis_d0a9f2af-ca3c-48ca-b567-7e7dca26beaa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_d0a9f2af-ca3c-48ca-b567-7e7dca26beaa_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_d0a9f2af-ca3c-48ca-b567-7e7dca26beaa" xlink:to="loc_srt_SegmentGeographicalDomain_d0a9f2af-ca3c-48ca-b567-7e7dca26beaa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_520d182e-f30c-4b7d-a513-30cba88239c0" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_d0a9f2af-ca3c-48ca-b567-7e7dca26beaa" xlink:to="loc_srt_SegmentGeographicalDomain_520d182e-f30c-4b7d-a513-30cba88239c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_4187d496-55eb-4a2e-8234-4c7ab592ad0b" xlink:href="https://xbrl.sec.gov/country/2021/country-2021.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_520d182e-f30c-4b7d-a513-30cba88239c0" xlink:to="loc_country_US_4187d496-55eb-4a2e-8234-4c7ab592ad0b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_5c4f6ea1-44a7-439b-b45b-88b8356c4089" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_520d182e-f30c-4b7d-a513-30cba88239c0" xlink:to="loc_us-gaap_NonUsMember_5c4f6ea1-44a7-439b-b45b-88b8356c4089" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/RelatedPartyTransactionsDetails" xlink:type="simple" xlink:href="asan-20220131.xsd#RelatedPartyTransactionsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/RelatedPartyTransactionsDetails" xlink:type="extended" id="i5a814b17d1534892a5a1014a30c38625_RelatedPartyTransactionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_3faeca94-5956-4656-8a58-2fb675dc8c45" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_34064df3-9401-4821-9204-4331b93ba09a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyTransactionAmountsOfTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_3faeca94-5956-4656-8a58-2fb675dc8c45" xlink:to="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_34064df3-9401-4821-9204-4331b93ba09a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_702dbedc-9fe5-478f-a654-717b522c9454" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_3faeca94-5956-4656-8a58-2fb675dc8c45" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_702dbedc-9fe5-478f-a654-717b522c9454" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_ce40ef39-7b2e-49cf-ae7f-71986533b1ce" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_702dbedc-9fe5-478f-a654-717b522c9454" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_ce40ef39-7b2e-49cf-ae7f-71986533b1ce" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_ce40ef39-7b2e-49cf-ae7f-71986533b1ce_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_ce40ef39-7b2e-49cf-ae7f-71986533b1ce" xlink:to="loc_us-gaap_RelatedPartyDomain_ce40ef39-7b2e-49cf-ae7f-71986533b1ce_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_fa666c3b-b248-434f-b43e-f7e21118d3a6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_ce40ef39-7b2e-49cf-ae7f-71986533b1ce" xlink:to="loc_us-gaap_RelatedPartyDomain_fa666c3b-b248-434f-b43e-f7e21118d3a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_AffiliatedEntityMember_ec815fbd-de6b-4480-9cff-10128a24a80d" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_AffiliatedEntityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_fa666c3b-b248-434f-b43e-f7e21118d3a6" xlink:to="loc_srt_AffiliatedEntityMember_ec815fbd-de6b-4480-9cff-10128a24a80d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_14120eb3-a3eb-43db-9895-b6b46d74e6bd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_702dbedc-9fe5-478f-a654-717b522c9454" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_14120eb3-a3eb-43db-9895-b6b46d74e6bd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_14120eb3-a3eb-43db-9895-b6b46d74e6bd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_14120eb3-a3eb-43db-9895-b6b46d74e6bd" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_14120eb3-a3eb-43db-9895-b6b46d74e6bd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_0b5ad717-06b5-4b5f-9d8b-9bf679628271" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_14120eb3-a3eb-43db-9895-b6b46d74e6bd" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_0b5ad717-06b5-4b5f-9d8b-9bf679628271" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_LeaseExpenseMember_eb3f7b83-9025-408e-93b3-145868e9fed3" xlink:href="asan-20220131.xsd#asan_LeaseExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_0b5ad717-06b5-4b5f-9d8b-9bf679628271" xlink:to="loc_asan_LeaseExpenseMember_eb3f7b83-9025-408e-93b3-145868e9fed3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_AdvertisingExpenseMember_b951ee07-74b6-4f39-aedf-afbb05280b01" xlink:href="asan-20220131.xsd#asan_AdvertisingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_0b5ad717-06b5-4b5f-9d8b-9bf679628271" xlink:to="loc_asan_AdvertisingExpenseMember_b951ee07-74b6-4f39-aedf-afbb05280b01" xlink:type="arc" order="1"/>
  </link:definitionLink>
</link:linkbase>
