<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2021 Workiva-->
<!--r:a7f491ec-9aff-4b5f-8b0a-15577abf4e1c,g:c1b0c025-0f1c-4d81-9380-847a825c3ca6-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://asana.com/role/Cover" xlink:type="simple" xlink:href="asan-20210131.xsd#Cover"/>
  <link:definitionLink xlink:role="http://asana.com/role/Cover" xlink:type="extended" id="if946e472abb84f3a94d9f61656ec5d89_Cover">
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_87158f3f-ae92-4366-b6f2-7a962dc81ee7" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_DocumentType_87158f3f-ae92-4366-b6f2-7a962dc81ee7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentAnnualReport_153d7db6-df4b-4863-ba16-aaceb02161f3" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentAnnualReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_DocumentAnnualReport_153d7db6-df4b-4863-ba16-aaceb02161f3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_e104cdb7-0ef7-4296-828a-1ae4a2a64305" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_DocumentTransitionReport_e104cdb7-0ef7-4296-828a-1ae4a2a64305" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_11ec24e6-9a39-42a3-8c5c-013409284526" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_DocumentPeriodEndDate_11ec24e6-9a39-42a3-8c5c-013409284526" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_03aca8bd-199b-417a-afe8-a0ab09d3931f" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_CurrentFiscalYearEndDate_03aca8bd-199b-417a-afe8-a0ab09d3931f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_5f6cdb12-3b39-4959-b509-aa1874cbb959" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityFileNumber_5f6cdb12-3b39-4959-b509-aa1874cbb959" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_17fc5237-c376-43dc-a283-455124a147c4" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityRegistrantName_17fc5237-c376-43dc-a283-455124a147c4" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_4b6733ab-9fe1-4081-9808-46d9faf7443e" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityIncorporationStateCountryCode_4b6733ab-9fe1-4081-9808-46d9faf7443e" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_90b057ff-ee30-4574-88d0-47d6cc009e95" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityTaxIdentificationNumber_90b057ff-ee30-4574-88d0-47d6cc009e95" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_c83a526e-1f67-4f50-a464-d14d374439c6" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityAddressAddressLine1_c83a526e-1f67-4f50-a464-d14d374439c6" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_2018bed3-2eb5-4d2e-8973-5367402763c4" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityAddressCityOrTown_2018bed3-2eb5-4d2e-8973-5367402763c4" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_ba2a9c37-0455-484c-8672-9ce17e1412b3" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityAddressStateOrProvince_ba2a9c37-0455-484c-8672-9ce17e1412b3" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_4eafc9bb-0b2f-445b-b4d7-d70e0d2a77ba" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityAddressPostalZipCode_4eafc9bb-0b2f-445b-b4d7-d70e0d2a77ba" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_4174077f-da1c-44cc-9a94-e81625a45b09" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_CityAreaCode_4174077f-da1c-44cc-9a94-e81625a45b09" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_6a5d4fb4-b48e-43cc-99c8-83342d2a156f" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_LocalPhoneNumber_6a5d4fb4-b48e-43cc-99c8-83342d2a156f" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_7cd48ef4-ee84-469c-a515-555ec1468e48" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_Security12bTitle_7cd48ef4-ee84-469c-a515-555ec1468e48" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_5fb36d2c-8337-4ffd-91f0-976cdb54ca1b" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_TradingSymbol_5fb36d2c-8337-4ffd-91f0-976cdb54ca1b" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_3a3445f1-0c13-420b-861e-9dafd1ab72b8" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_SecurityExchangeName_3a3445f1-0c13-420b-861e-9dafd1ab72b8" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityWellKnownSeasonedIssuer_8180fa8e-4b1e-463f-a98c-5516fc2e3f5b" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityWellKnownSeasonedIssuer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityWellKnownSeasonedIssuer_8180fa8e-4b1e-463f-a98c-5516fc2e3f5b" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityVoluntaryFilers_6667cf37-6c81-45a7-a955-455ba8451e38" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityVoluntaryFilers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityVoluntaryFilers_6667cf37-6c81-45a7-a955-455ba8451e38" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_1b115b53-4af0-4e27-93dc-e22c547ed805" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityCurrentReportingStatus_1b115b53-4af0-4e27-93dc-e22c547ed805" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_db65b9f4-1203-48cb-8802-ad1bf4c781c2" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityInteractiveDataCurrent_db65b9f4-1203-48cb-8802-ad1bf4c781c2" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_f3fc225a-dfcd-41d4-b078-5c607fc143e7" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityFilerCategory_f3fc225a-dfcd-41d4-b078-5c607fc143e7" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_4f3c945c-c9f3-4921-85f1-01a3b41eab57" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntitySmallBusiness_4f3c945c-c9f3-4921-85f1-01a3b41eab57" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_ce04e2fc-307b-45df-9303-a7ee5b2b5e97" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityEmergingGrowthCompany_ce04e2fc-307b-45df-9303-a7ee5b2b5e97" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityExTransitionPeriod_cb84d0e9-1d02-4849-9a57-66ba0ae1f52f" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityExTransitionPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityExTransitionPeriod_cb84d0e9-1d02-4849-9a57-66ba0ae1f52f" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_fbd765ae-cfb8-4f08-9162-1c89705b67fd" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityShellCompany_fbd765ae-cfb8-4f08-9162-1c89705b67fd" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityPublicFloat_6d31b3ec-e349-4706-9176-74c65ea3ee58" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityPublicFloat"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityPublicFloat_6d31b3ec-e349-4706-9176-74c65ea3ee58" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_25e029ab-b82c-4b59-8a25-ed1b96ff158f" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_25e029ab-b82c-4b59-8a25-ed1b96ff158f" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentsIncorporatedByReferenceTextBlock_f1d6f4d0-f13a-4d87-aa22-746f826720d8" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentsIncorporatedByReferenceTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_DocumentsIncorporatedByReferenceTextBlock_f1d6f4d0-f13a-4d87-aa22-746f826720d8" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_b0ba7145-0b87-4ccd-89e6-d36fe7e2ad13" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_EntityCentralIndexKey_b0ba7145-0b87-4ccd-89e6-d36fe7e2ad13" xlink:type="arc" order="30"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_1dadbcf4-c145-446f-8c63-2815efc34369" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_DocumentFiscalYearFocus_1dadbcf4-c145-446f-8c63-2815efc34369" xlink:type="arc" order="31"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_37673cab-28bc-4d36-a103-e4587266657f" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_DocumentFiscalPeriodFocus_37673cab-28bc-4d36-a103-e4587266657f" xlink:type="arc" order="32"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_9d8a278c-fbb9-4fa1-97a9-cca20aa260a9" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_AmendmentFlag_9d8a278c-fbb9-4fa1-97a9-cca20aa260a9" xlink:type="arc" order="33"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationTable_e0fce438-3310-4bfd-9671-1adf955456cb" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentInformationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_DocumentInformationLineItems_62876f06-f4e2-41b8-be35-aacd50bbc662" xlink:to="loc_dei_DocumentInformationTable_e0fce438-3310-4bfd-9671-1adf955456cb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_19fa069b-63f0-4f8b-bae8-e50feddd19ea" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_DocumentInformationTable_e0fce438-3310-4bfd-9671-1adf955456cb" xlink:to="loc_us-gaap_StatementClassOfStockAxis_19fa069b-63f0-4f8b-bae8-e50feddd19ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_19fa069b-63f0-4f8b-bae8-e50feddd19ea_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_19fa069b-63f0-4f8b-bae8-e50feddd19ea" xlink:to="loc_us-gaap_ClassOfStockDomain_19fa069b-63f0-4f8b-bae8-e50feddd19ea_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_401b3609-2bc8-498c-bc01-771c58b8685d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_19fa069b-63f0-4f8b-bae8-e50feddd19ea" xlink:to="loc_us-gaap_ClassOfStockDomain_401b3609-2bc8-498c-bc01-771c58b8685d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_93e13fc8-3320-4b5b-bf00-d946d63227ff" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_401b3609-2bc8-498c-bc01-771c58b8685d" xlink:to="loc_us-gaap_CommonClassAMember_93e13fc8-3320-4b5b-bf00-d946d63227ff" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_70b4f882-fb05-4da5-9750-e582c7f42c82" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_401b3609-2bc8-498c-bc01-771c58b8685d" xlink:to="loc_us-gaap_CommonClassBMember_70b4f882-fb05-4da5-9750-e582c7f42c82" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/ConsolidatedBalanceSheets" xlink:type="simple" xlink:href="asan-20210131.xsd#ConsolidatedBalanceSheets"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConsolidatedBalanceSheets" xlink:type="extended" id="i976f4d82bdda4548b93b9ab6f008f69f_ConsolidatedBalanceSheets"/>
  <link:roleRef roleURI="http://asana.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:type="simple" xlink:href="asan-20210131.xsd#ConsolidatedBalanceSheetsParenthetical"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:type="extended" id="ide23a2d624064686bb0da37f36ea3c50_ConsolidatedBalanceSheetsParenthetical">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_6d8d01d2-3aaa-4380-883f-4423cde7ec28" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesOutstanding_16edce88-ecfa-438c-b6b3-14be4b8b42d7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquitySharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_6d8d01d2-3aaa-4380-883f-4423cde7ec28" xlink:to="loc_us-gaap_TemporaryEquitySharesOutstanding_16edce88-ecfa-438c-b6b3-14be4b8b42d7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_7542a5d2-188c-4ced-9c12-fdbcd7519b42" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_6d8d01d2-3aaa-4380-883f-4423cde7ec28" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_7542a5d2-188c-4ced-9c12-fdbcd7519b42" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesAuthorized_76d626c8-b3c5-4e0b-b46d-712fca7685dc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquitySharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_6d8d01d2-3aaa-4380-883f-4423cde7ec28" xlink:to="loc_us-gaap_TemporaryEquitySharesAuthorized_76d626c8-b3c5-4e0b-b46d-712fca7685dc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityLiquidationPreference_f98ffa5a-3fc7-4faa-a7c1-2273d8fd858b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquityLiquidationPreference"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_6d8d01d2-3aaa-4380-883f-4423cde7ec28" xlink:to="loc_us-gaap_TemporaryEquityLiquidationPreference_f98ffa5a-3fc7-4faa-a7c1-2273d8fd858b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityParOrStatedValuePerShare_c89b1803-554b-4bb2-979e-f72844dd420e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquityParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_6d8d01d2-3aaa-4380-883f-4423cde7ec28" xlink:to="loc_us-gaap_TemporaryEquityParOrStatedValuePerShare_c89b1803-554b-4bb2-979e-f72844dd420e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_3a495e09-fc56-43a0-8e0f-34b0cc8c3c15" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_6d8d01d2-3aaa-4380-883f-4423cde7ec28" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_3a495e09-fc56-43a0-8e0f-34b0cc8c3c15" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_afbc389b-f553-439a-a834-222c167b8746" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_6d8d01d2-3aaa-4380-883f-4423cde7ec28" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_afbc389b-f553-439a-a834-222c167b8746" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_71159397-f7f1-4d36-b1c2-80bb4d23e431" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_6d8d01d2-3aaa-4380-883f-4423cde7ec28" xlink:to="loc_us-gaap_StatementTable_71159397-f7f1-4d36-b1c2-80bb4d23e431" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RestatementAxis_44bdcf1f-ace4-4849-9d09-ebcdab055357" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RestatementAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_71159397-f7f1-4d36-b1c2-80bb4d23e431" xlink:to="loc_srt_RestatementAxis_44bdcf1f-ace4-4849-9d09-ebcdab055357" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RestatementDomain_44bdcf1f-ace4-4849-9d09-ebcdab055357_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RestatementDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RestatementAxis_44bdcf1f-ace4-4849-9d09-ebcdab055357" xlink:to="loc_srt_RestatementDomain_44bdcf1f-ace4-4849-9d09-ebcdab055357_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RestatementDomain_4c04d9bf-e6c1-4da1-a1d0-c7011e3e6e13" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RestatementDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RestatementAxis_44bdcf1f-ace4-4849-9d09-ebcdab055357" xlink:to="loc_srt_RestatementDomain_4c04d9bf-e6c1-4da1-a1d0-c7011e3e6e13" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_b41fedea-cd34-4eee-a1fd-63cbfba00102" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_71159397-f7f1-4d36-b1c2-80bb4d23e431" xlink:to="loc_us-gaap_StatementClassOfStockAxis_b41fedea-cd34-4eee-a1fd-63cbfba00102" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_b41fedea-cd34-4eee-a1fd-63cbfba00102_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b41fedea-cd34-4eee-a1fd-63cbfba00102" xlink:to="loc_us-gaap_ClassOfStockDomain_b41fedea-cd34-4eee-a1fd-63cbfba00102_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_f9d143bd-95c2-41f2-a5df-170842fccfd1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b41fedea-cd34-4eee-a1fd-63cbfba00102" xlink:to="loc_us-gaap_ClassOfStockDomain_f9d143bd-95c2-41f2-a5df-170842fccfd1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_8ccbdbda-377e-433a-8c62-180a40e789b8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_71159397-f7f1-4d36-b1c2-80bb4d23e431" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_8ccbdbda-377e-433a-8c62-180a40e789b8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_8ccbdbda-377e-433a-8c62-180a40e789b8_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_8ccbdbda-377e-433a-8c62-180a40e789b8" xlink:to="loc_us-gaap_EquityComponentDomain_8ccbdbda-377e-433a-8c62-180a40e789b8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_71ecad52-579c-43a1-8ced-80d1a302655f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_8ccbdbda-377e-433a-8c62-180a40e789b8" xlink:to="loc_us-gaap_EquityComponentDomain_71ecad52-579c-43a1-8ced-80d1a302655f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_e6359dee-f1b0-46d7-a812-7aaaef2335f5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_71ecad52-579c-43a1-8ced-80d1a302655f" xlink:to="loc_us-gaap_CommonStockMember_e6359dee-f1b0-46d7-a812-7aaaef2335f5" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/ConsolidatedStatementsofOperations" xlink:type="simple" xlink:href="asan-20210131.xsd#ConsolidatedStatementsofOperations"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConsolidatedStatementsofOperations" xlink:type="extended" id="i3103cd6eb3e24341aaffbc76902f40d4_ConsolidatedStatementsofOperations"/>
  <link:roleRef roleURI="http://asana.com/role/ConsolidatedStatementsofComprehensiveLoss" xlink:type="simple" xlink:href="asan-20210131.xsd#ConsolidatedStatementsofComprehensiveLoss"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConsolidatedStatementsofComprehensiveLoss" xlink:type="extended" id="id917c44ea01e4257b93495cc61a5796a_ConsolidatedStatementsofComprehensiveLoss"/>
  <link:roleRef roleURI="http://asana.com/role/ConsolidatedStatementsofRedeemableConvertiblePreferredStockandStockholdersDeficit" xlink:type="simple" xlink:href="asan-20210131.xsd#ConsolidatedStatementsofRedeemableConvertiblePreferredStockandStockholdersDeficit"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConsolidatedStatementsofRedeemableConvertiblePreferredStockandStockholdersDeficit" xlink:type="extended" id="ia546c6e4b8c9437e9353500e7d5ab4e6_ConsolidatedStatementsofRedeemableConvertiblePreferredStockandStockholdersDeficit">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_3da307dd-4a88-4d47-87f4-7f5bd2f492d4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_3da307dd-4a88-4d47-87f4-7f5bd2f492d4" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_93ab50ff-1996-493f-9c73-689e7165930f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_93ab50ff-1996-493f-9c73-689e7165930f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_aa5b08cf-3786-4c64-b6bf-3beb78f79d84" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_StockholdersEquity_aa5b08cf-3786-4c64-b6bf-3beb78f79d84" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_c90a6774-c406-4b99-8919-225d7bc46c9d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_c90a6774-c406-4b99-8919-225d7bc46c9d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_c424905f-c139-4d21-be50-e71cd5738f1d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_c424905f-c139-4d21-be50-e71cd5738f1d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions_8ed254ab-e24d-415c-95a9-3671689c9fee" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions_8ed254ab-e24d-415c-95a9-3671689c9fee" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodShares_dfa750a0-30d9-4c17-9329-850355f3d469" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockRepurchasedDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodShares_dfa750a0-30d9-4c17-9329-850355f3d469" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodValue_348259e6-44dc-4d19-b80d-8354d9741c50" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockRepurchasedDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodValue_348259e6-44dc-4d19-b80d-8354d9741c50" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_a3d2c17a-4751-4a3c-8cfa-3dd84f6b2967" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_a3d2c17a-4751-4a3c-8cfa-3dd84f6b2967" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_80927c18-5647-466f-8d0d-2d4252570ec3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_80927c18-5647-466f-8d0d-2d4252570ec3" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_52508b8d-4d98-49a9-a85f-5ab5398e0383" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_52508b8d-4d98-49a9-a85f-5ab5398e0383" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_f060c993-f5c9-44bd-abc4-cd478c8718e1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_f060c993-f5c9-44bd-abc4-cd478c8718e1" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax_a2e6db79-e8bd-46ca-84cf-13ef875a9bbe" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax_a2e6db79-e8bd-46ca-84cf-13ef875a9bbe" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalEquityComponentOfConvertibleDebt_e977f047-3c48-4b06-82e7-b01e85688f7f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalEquityComponentOfConvertibleDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalEquityComponentOfConvertibleDebt_e977f047-3c48-4b06-82e7-b01e85688f7f" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent_8ea84a1b-e2ee-40df-8c92-f381b18703af" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent_8ea84a1b-e2ee-40df-8c92-f381b18703af" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_a4835b3e-6a01-4fe0-af1c-91a8d07739a2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_acb3c495-5ea8-4f04-a6c2-43f0287a8ceb" xlink:to="loc_us-gaap_NetIncomeLoss_a4835b3e-6a01-4fe0-af1c-91a8d07739a2" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_be856175-dcc8-453a-a19d-f77505851d4c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_184041e9-bd28-4f16-9452-fc6ece99cec8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_8b44a73e-f72b-47cd-9599-824a9790b8cb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInTemporaryEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_3da307dd-4a88-4d47-87f4-7f5bd2f492d4" xlink:to="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_8b44a73e-f72b-47cd-9599-824a9790b8cb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesOutstanding_51bc9ab1-0cfe-49b1-8328-50f54361b9f8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquitySharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_8b44a73e-f72b-47cd-9599-824a9790b8cb" xlink:to="loc_us-gaap_TemporaryEquitySharesOutstanding_51bc9ab1-0cfe-49b1-8328-50f54361b9f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_c517d5b6-ddfc-4cb9-9235-90cdb1871802" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquityCarryingAmountAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_8b44a73e-f72b-47cd-9599-824a9790b8cb" xlink:to="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_c517d5b6-ddfc-4cb9-9235-90cdb1871802" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_ebc917a7-64de-4233-be43-6f0b0c279afc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_8b44a73e-f72b-47cd-9599-824a9790b8cb" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_ebc917a7-64de-4233-be43-6f0b0c279afc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationsOfTemporaryToPermanentEquity_ae7eb5d6-ec5c-41f7-b3f1-79c8c9b23fdb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ReclassificationsOfTemporaryToPermanentEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_8b44a73e-f72b-47cd-9599-824a9790b8cb" xlink:to="loc_us-gaap_ReclassificationsOfTemporaryToPermanentEquity_ae7eb5d6-ec5c-41f7-b3f1-79c8c9b23fdb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesOutstanding_936cc2b7-a55c-4012-8f46-7dbc4af85b90" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquitySharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_4e40667b-6d9c-4734-b6db-c48bd564dfe5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquityCarryingAmountAttributableToParent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_9376c7be-5e7d-42a0-80b7-eaa847b4da2f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_3da307dd-4a88-4d47-87f4-7f5bd2f492d4" xlink:to="loc_us-gaap_StatementTable_9376c7be-5e7d-42a0-80b7-eaa847b4da2f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_729480fc-eb69-44bf-aa64-efd3f49b680d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_9376c7be-5e7d-42a0-80b7-eaa847b4da2f" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_729480fc-eb69-44bf-aa64-efd3f49b680d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_729480fc-eb69-44bf-aa64-efd3f49b680d_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_729480fc-eb69-44bf-aa64-efd3f49b680d" xlink:to="loc_us-gaap_EquityComponentDomain_729480fc-eb69-44bf-aa64-efd3f49b680d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_fa653d9a-26e3-49ec-849a-4a705790b803" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_729480fc-eb69-44bf-aa64-efd3f49b680d" xlink:to="loc_us-gaap_EquityComponentDomain_fa653d9a-26e3-49ec-849a-4a705790b803" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_fc8ab4ca-cfff-48c3-8377-89a07e6970a3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_fa653d9a-26e3-49ec-849a-4a705790b803" xlink:to="loc_us-gaap_CommonStockMember_fc8ab4ca-cfff-48c3-8377-89a07e6970a3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_53caa9dd-3cc6-4eb5-beaf-96a25b547447" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_fa653d9a-26e3-49ec-849a-4a705790b803" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_53caa9dd-3cc6-4eb5-beaf-96a25b547447" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_d738cccb-aafc-4eaf-b6c1-d16824853312" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_fa653d9a-26e3-49ec-849a-4a705790b803" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_d738cccb-aafc-4eaf-b6c1-d16824853312" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_2ab3ec36-e581-42b6-a136-dc70f22af6a7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_fa653d9a-26e3-49ec-849a-4a705790b803" xlink:to="loc_us-gaap_RetainedEarningsMember_2ab3ec36-e581-42b6-a136-dc70f22af6a7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_f8f1355a-663f-48b1-ac2d-8dc034f751ac" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_9376c7be-5e7d-42a0-80b7-eaa847b4da2f" xlink:to="loc_us-gaap_StatementClassOfStockAxis_f8f1355a-663f-48b1-ac2d-8dc034f751ac" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_f8f1355a-663f-48b1-ac2d-8dc034f751ac_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_f8f1355a-663f-48b1-ac2d-8dc034f751ac" xlink:to="loc_us-gaap_ClassOfStockDomain_f8f1355a-663f-48b1-ac2d-8dc034f751ac_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_bbf69c82-2aa7-41ee-8ebb-f887941b9dbc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_f8f1355a-663f-48b1-ac2d-8dc034f751ac" xlink:to="loc_us-gaap_ClassOfStockDomain_bbf69c82-2aa7-41ee-8ebb-f887941b9dbc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RedeemableConvertiblePreferredStockMember_5316e721-d74e-4b2e-9150-a56b602b62fd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RedeemableConvertiblePreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_bbf69c82-2aa7-41ee-8ebb-f887941b9dbc" xlink:to="loc_us-gaap_RedeemableConvertiblePreferredStockMember_5316e721-d74e-4b2e-9150-a56b602b62fd" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/ConsolidatedStatementsofCashFlows" xlink:type="simple" xlink:href="asan-20210131.xsd#ConsolidatedStatementsofCashFlows"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConsolidatedStatementsofCashFlows" xlink:type="extended" id="i16f45eeb36ad40f7a8284142f117314f_ConsolidatedStatementsofCashFlows"/>
  <link:roleRef roleURI="http://asana.com/role/Organization" xlink:type="simple" xlink:href="asan-20210131.xsd#Organization"/>
  <link:definitionLink xlink:role="http://asana.com/role/Organization" xlink:type="extended" id="ie5f4f69f932e493da7131309eb9b64ac_Organization"/>
  <link:roleRef roleURI="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPolicies" xlink:type="simple" xlink:href="asan-20210131.xsd#BasisofPresentationandSummaryofSignificantAccountingPolicies"/>
  <link:definitionLink xlink:role="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPolicies" xlink:type="extended" id="iacedcd6308ce4552b0e7d46894557b78_BasisofPresentationandSummaryofSignificantAccountingPolicies"/>
  <link:roleRef roleURI="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesPolicies" xlink:type="simple" xlink:href="asan-20210131.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesPolicies"/>
  <link:definitionLink xlink:role="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesPolicies" xlink:type="extended" id="if950f97d00264957baff003fde0c416e_BasisofPresentationandSummaryofSignificantAccountingPoliciesPolicies"/>
  <link:roleRef roleURI="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesTables" xlink:type="simple" xlink:href="asan-20210131.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesTables" xlink:type="extended" id="ibc8fec7739c6473aad63f710556c26a7_BasisofPresentationandSummaryofSignificantAccountingPoliciesTables"/>
  <link:roleRef roleURI="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesNarrativeDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesNarrativeDetails" xlink:type="extended" id="i5bef3aea5c3047998cd66f710d5bf72a_BasisofPresentationandSummaryofSignificantAccountingPoliciesNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfessionalFees_571f1644-fe79-42b9-8260-0daf37eda29e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProfessionalFees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_ProfessionalFees_571f1644-fe79-42b9-8260-0daf37eda29e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_af6a52ac-2d8c-4f60-9a94-d58b84d41b9f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_af6a52ac-2d8c-4f60-9a94-d58b84d41b9f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdvertisingExpense_4a6296a5-6a93-48a2-8245-7de9c1c98afd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdvertisingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_AdvertisingExpense_4a6296a5-6a93-48a2-8245-7de9c1c98afd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCash_f88c7295-26ab-4218-be89-58889f74f12f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_RestrictedCash_f88c7295-26ab-4218-be89-58889f74f12f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTerm_95e4ea8a-9f4f-435a-8fb7-2d6783335753" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTerm_95e4ea8a-9f4f-435a-8fb7-2d6783335753" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MarketableSecuritiesRealizedGainLoss_a241a465-aaff-452d-b02d-23ebe4999f8a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MarketableSecuritiesRealizedGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_MarketableSecuritiesRealizedGainLoss_a241a465-aaff-452d-b02d-23ebe4999f8a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_9da6bdee-6484-4b16-a166-f152e2f5b97c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_9da6bdee-6484-4b16-a166-f152e2f5b97c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_ca78a9d3-a633-4349-b13e-a0ee749afc05" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_ca78a9d3-a633-4349-b13e-a0ee749afc05" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairmentOfLongLivedAssetsHeldForUse_c510c5a6-a360-4c0b-98bf-618811cac450" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ImpairmentOfLongLivedAssetsHeldForUse"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_ImpairmentOfLongLivedAssetsHeldForUse_c510c5a6-a360-4c0b-98bf-618811cac450" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesIssued_1e20cb9b-3f87-498f-93b1-c963feb916d1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_PreferredStockSharesIssued_1e20cb9b-3f87-498f-93b1-c963feb916d1" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6b06c9f5-1ce3-4ad8-8657-09a3ea403999" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3ab5c58b-0e68-4da2-b210-09beab9445f7" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6b06c9f5-1ce3-4ad8-8657-09a3ea403999" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_a315ab8b-a165-46b4-a72c-fadbe3ff786e" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6b06c9f5-1ce3-4ad8-8657-09a3ea403999" xlink:to="loc_srt_RangeAxis_a315ab8b-a165-46b4-a72c-fadbe3ff786e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_a315ab8b-a165-46b4-a72c-fadbe3ff786e_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_a315ab8b-a165-46b4-a72c-fadbe3ff786e" xlink:to="loc_srt_RangeMember_a315ab8b-a165-46b4-a72c-fadbe3ff786e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_9e8a520f-c9de-4a7b-91ef-33a154e8632c" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_a315ab8b-a165-46b4-a72c-fadbe3ff786e" xlink:to="loc_srt_RangeMember_9e8a520f-c9de-4a7b-91ef-33a154e8632c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_1a0fa426-100f-494b-a01c-52d4d4988c10" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_9e8a520f-c9de-4a7b-91ef-33a154e8632c" xlink:to="loc_srt_MinimumMember_1a0fa426-100f-494b-a01c-52d4d4988c10" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_d4a6f9bb-453d-4bf2-9a6f-11fffa181670" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_9e8a520f-c9de-4a7b-91ef-33a154e8632c" xlink:to="loc_srt_MaximumMember_d4a6f9bb-453d-4bf2-9a6f-11fffa181670" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_b5297116-31f2-4101-8686-f1de6afdce43" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6b06c9f5-1ce3-4ad8-8657-09a3ea403999" xlink:to="loc_us-gaap_StatementClassOfStockAxis_b5297116-31f2-4101-8686-f1de6afdce43" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_b5297116-31f2-4101-8686-f1de6afdce43_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b5297116-31f2-4101-8686-f1de6afdce43" xlink:to="loc_us-gaap_ClassOfStockDomain_b5297116-31f2-4101-8686-f1de6afdce43_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_d18d0746-f101-426f-9ca8-2fb4f9a9e21f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b5297116-31f2-4101-8686-f1de6afdce43" xlink:to="loc_us-gaap_ClassOfStockDomain_d18d0746-f101-426f-9ca8-2fb4f9a9e21f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesBRedeemableConvertiblePreferredStockMember_fdb12483-3f59-4406-a6c2-9167f7ae3bf1" xlink:href="asan-20210131.xsd#asan_SeriesBRedeemableConvertiblePreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_d18d0746-f101-426f-9ca8-2fb4f9a9e21f" xlink:to="loc_asan_SeriesBRedeemableConvertiblePreferredStockMember_fdb12483-3f59-4406-a6c2-9167f7ae3bf1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MajorCustomersAxis_27568f99-fa8a-4748-a30c-19234fd09826" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6b06c9f5-1ce3-4ad8-8657-09a3ea403999" xlink:to="loc_srt_MajorCustomersAxis_27568f99-fa8a-4748-a30c-19234fd09826" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_27568f99-fa8a-4748-a30c-19234fd09826_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MajorCustomersAxis_27568f99-fa8a-4748-a30c-19234fd09826" xlink:to="loc_srt_NameOfMajorCustomerDomain_27568f99-fa8a-4748-a30c-19234fd09826_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_960b21e2-73eb-487c-9052-3065004e0b26" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MajorCustomersAxis_27568f99-fa8a-4748-a30c-19234fd09826" xlink:to="loc_srt_NameOfMajorCustomerDomain_960b21e2-73eb-487c-9052-3065004e0b26" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_CustomerOneMember_9e27d04b-640f-4ab4-9001-c15faa2fed53" xlink:href="asan-20210131.xsd#asan_CustomerOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_960b21e2-73eb-487c-9052-3065004e0b26" xlink:to="loc_asan_CustomerOneMember_9e27d04b-640f-4ab4-9001-c15faa2fed53" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_4c85b1b6-e079-4527-a070-9183beb65da3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6b06c9f5-1ce3-4ad8-8657-09a3ea403999" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_4c85b1b6-e079-4527-a070-9183beb65da3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_4c85b1b6-e079-4527-a070-9183beb65da3_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_4c85b1b6-e079-4527-a070-9183beb65da3" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_4c85b1b6-e079-4527-a070-9183beb65da3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_8f6d7ccb-9334-4feb-b02b-43607b699f39" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_4c85b1b6-e079-4527-a070-9183beb65da3" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_8f6d7ccb-9334-4feb-b02b-43607b699f39" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerMember_bf6e4838-7171-4c6c-9252-6bdcb8fdcf99" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_8f6d7ccb-9334-4feb-b02b-43607b699f39" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerMember_bf6e4838-7171-4c6c-9252-6bdcb8fdcf99" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_d9531058-64cd-4b53-b538-0ae6d29f8b8e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6b06c9f5-1ce3-4ad8-8657-09a3ea403999" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_d9531058-64cd-4b53-b538-0ae6d29f8b8e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_d9531058-64cd-4b53-b538-0ae6d29f8b8e_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_d9531058-64cd-4b53-b538-0ae6d29f8b8e" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_d9531058-64cd-4b53-b538-0ae6d29f8b8e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_5513271f-4d92-43e3-b808-4df909a94041" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_d9531058-64cd-4b53-b538-0ae6d29f8b8e" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_5513271f-4d92-43e3-b808-4df909a94041" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerConcentrationRiskMember_baeda22c-2f8a-40e0-bfc7-2bc7758e4f35" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CustomerConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_5513271f-4d92-43e3-b808-4df909a94041" xlink:to="loc_us-gaap_CustomerConcentrationRiskMember_baeda22c-2f8a-40e0-bfc7-2bc7758e4f35" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesCashandCashEquivalentsDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesCashandCashEquivalentsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesCashandCashEquivalentsDetails" xlink:type="extended" id="i1253173786e1496ab2ae2cedd32a67eb_BasisofPresentationandSummaryofSignificantAccountingPoliciesCashandCashEquivalentsDetails"/>
  <link:roleRef roleURI="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesPropertyAndEquipmentDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesPropertyAndEquipmentDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesPropertyAndEquipmentDetails" xlink:type="extended" id="i2c05783cd58a4d3e91f44c00f402806f_BasisofPresentationandSummaryofSignificantAccountingPoliciesPropertyAndEquipmentDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_83da651f-9a56-40b6-9d82-6dda3a059376" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_a1f06560-f1b8-4022-862c-74658e32303f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_83da651f-9a56-40b6-9d82-6dda3a059376" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_a1f06560-f1b8-4022-862c-74658e32303f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_82454f09-6042-4e63-97b4-7a11e856345d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_83da651f-9a56-40b6-9d82-6dda3a059376" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_82454f09-6042-4e63-97b4-7a11e856345d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_10f8cdce-5f33-4ad3-9289-c0e715038101" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_82454f09-6042-4e63-97b4-7a11e856345d" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_10f8cdce-5f33-4ad3-9289-c0e715038101" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_10f8cdce-5f33-4ad3-9289-c0e715038101_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_10f8cdce-5f33-4ad3-9289-c0e715038101" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_10f8cdce-5f33-4ad3-9289-c0e715038101_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_cfd18704-f238-48be-b9ab-04a12e444c7b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_10f8cdce-5f33-4ad3-9289-c0e715038101" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_cfd18704-f238-48be-b9ab-04a12e444c7b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerEquipmentMember_93943989-238c-4403-bcca-9fb3d7158e3a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_cfd18704-f238-48be-b9ab-04a12e444c7b" xlink:to="loc_us-gaap_ComputerEquipmentMember_93943989-238c-4403-bcca-9fb3d7158e3a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_6878597f-3152-43a3-8c17-5276004f9c30" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FurnitureAndFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_cfd18704-f238-48be-b9ab-04a12e444c7b" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_6878597f-3152-43a3-8c17-5276004f9c30" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SoftwareAndSoftwareDevelopmentCostsMember_9b9114c0-7b4b-4b2b-a2b9-eec69104c286" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SoftwareAndSoftwareDevelopmentCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_cfd18704-f238-48be-b9ab-04a12e444c7b" xlink:to="loc_us-gaap_SoftwareAndSoftwareDevelopmentCostsMember_9b9114c0-7b4b-4b2b-a2b9-eec69104c286" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_b341f3ec-f202-4d7b-9d36-2d0406cfc701" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_82454f09-6042-4e63-97b4-7a11e856345d" xlink:to="loc_srt_RangeAxis_b341f3ec-f202-4d7b-9d36-2d0406cfc701" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_b341f3ec-f202-4d7b-9d36-2d0406cfc701_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_b341f3ec-f202-4d7b-9d36-2d0406cfc701" xlink:to="loc_srt_RangeMember_b341f3ec-f202-4d7b-9d36-2d0406cfc701_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_0dbdc6b2-3536-42c4-866d-52ebdb01282a" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_b341f3ec-f202-4d7b-9d36-2d0406cfc701" xlink:to="loc_srt_RangeMember_0dbdc6b2-3536-42c4-866d-52ebdb01282a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_f2e17840-8507-4580-a1eb-5c603c99d2af" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_0dbdc6b2-3536-42c4-866d-52ebdb01282a" xlink:to="loc_srt_MinimumMember_f2e17840-8507-4580-a1eb-5c603c99d2af" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_83c50876-4322-45e4-961a-c057bd0783d3" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_0dbdc6b2-3536-42c4-866d-52ebdb01282a" xlink:to="loc_srt_MaximumMember_83c50876-4322-45e4-961a-c057bd0783d3" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesRecentlyAdoptedAccountingPoliciesDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesRecentlyAdoptedAccountingPoliciesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesRecentlyAdoptedAccountingPoliciesDetails" xlink:type="extended" id="i9b0f6d093f3f4380a6c6bda80d822d8b_BasisofPresentationandSummaryofSignificantAccountingPoliciesRecentlyAdoptedAccountingPoliciesDetails"/>
  <link:roleRef roleURI="http://asana.com/role/Revenue" xlink:type="simple" xlink:href="asan-20210131.xsd#Revenue"/>
  <link:definitionLink xlink:role="http://asana.com/role/Revenue" xlink:type="extended" id="iba9c81dae3de4f8bb9446c6e2a71cb30_Revenue"/>
  <link:roleRef roleURI="http://asana.com/role/RevenueTables" xlink:type="simple" xlink:href="asan-20210131.xsd#RevenueTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/RevenueTables" xlink:type="extended" id="i73f3ce3763264f9c9e2f483f15bdcf0e_RevenueTables"/>
  <link:roleRef roleURI="http://asana.com/role/RevenueNarrativeDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#RevenueNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/RevenueNarrativeDetails" xlink:type="extended" id="iaa74fe9d828147dca72cc479443e2c8b_RevenueNarrativeDetails"/>
  <link:roleRef roleURI="http://asana.com/role/RevenuePerformanceObligationsDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#RevenuePerformanceObligationsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/RevenuePerformanceObligationsDetails" xlink:type="extended" id="ie19976244bee4c17b38f3e49a7354bda_RevenuePerformanceObligationsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_d0796b6a-75ec-477d-9738-90806ff04e29" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_8c31e8a9-dc26-4d2d-a476-bcb789d4d528" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_d0796b6a-75ec-477d-9738-90806ff04e29" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_8c31e8a9-dc26-4d2d-a476-bcb789d4d528" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_3e6f0674-d7cd-4384-b7f3-9ef3dceff1d2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_d0796b6a-75ec-477d-9738-90806ff04e29" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_3e6f0674-d7cd-4384-b7f3-9ef3dceff1d2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_62d75ab5-8cc4-4747-bc31-776c595d54e8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_d0796b6a-75ec-477d-9738-90806ff04e29" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_62d75ab5-8cc4-4747-bc31-776c595d54e8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_981152ea-2ac9-4e3d-9bf0-518f0f0d5fe9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_d0796b6a-75ec-477d-9738-90806ff04e29" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_981152ea-2ac9-4e3d-9bf0-518f0f0d5fe9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis_5814a3e1-f1b4-4b5c-a4c3-0e3a2e2de481" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_981152ea-2ac9-4e3d-9bf0-518f0f0d5fe9" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis_5814a3e1-f1b4-4b5c-a4c3-0e3a2e2de481" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/RevenuePerformanceObligationsDetails_1" xlink:type="simple" xlink:href="asan-20210131.xsd#RevenuePerformanceObligationsDetails_1"/>
  <link:definitionLink xlink:role="http://asana.com/role/RevenuePerformanceObligationsDetails_1" xlink:type="extended" id="id6f72ba220284db18a36ae1b78af42ce_RevenuePerformanceObligationsDetails_1">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_d0796b6a-75ec-477d-9738-90806ff04e29" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_8c31e8a9-dc26-4d2d-a476-bcb789d4d528" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_d0796b6a-75ec-477d-9738-90806ff04e29" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_8c31e8a9-dc26-4d2d-a476-bcb789d4d528" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_3e6f0674-d7cd-4384-b7f3-9ef3dceff1d2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_d0796b6a-75ec-477d-9738-90806ff04e29" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_3e6f0674-d7cd-4384-b7f3-9ef3dceff1d2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_62d75ab5-8cc4-4747-bc31-776c595d54e8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_d0796b6a-75ec-477d-9738-90806ff04e29" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_62d75ab5-8cc4-4747-bc31-776c595d54e8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_981152ea-2ac9-4e3d-9bf0-518f0f0d5fe9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_d0796b6a-75ec-477d-9738-90806ff04e29" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_981152ea-2ac9-4e3d-9bf0-518f0f0d5fe9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/RevenueDeferredContractAcquisitionCostsActivityDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#RevenueDeferredContractAcquisitionCostsActivityDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/RevenueDeferredContractAcquisitionCostsActivityDetails" xlink:type="extended" id="ib4c0ed9004624a01a023f5eb9a77831f_RevenueDeferredContractAcquisitionCostsActivityDetails"/>
  <link:roleRef roleURI="http://asana.com/role/FairValueMeasurements" xlink:type="simple" xlink:href="asan-20210131.xsd#FairValueMeasurements"/>
  <link:definitionLink xlink:role="http://asana.com/role/FairValueMeasurements" xlink:type="extended" id="i1b17fcf1802d418ea58e864b4bfe9dde_FairValueMeasurements"/>
  <link:roleRef roleURI="http://asana.com/role/FairValueMeasurementsTables" xlink:type="simple" xlink:href="asan-20210131.xsd#FairValueMeasurementsTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/FairValueMeasurementsTables" xlink:type="extended" id="if5ab89ef7ae64b588d6d54f8b3d0c0fa_FairValueMeasurementsTables"/>
  <link:roleRef roleURI="http://asana.com/role/FairValueMeasurementsFairValueHierarchyDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#FairValueMeasurementsFairValueHierarchyDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/FairValueMeasurementsFairValueHierarchyDetails" xlink:type="extended" id="ic5a456c48f0b432e97b569340677c15f_FairValueMeasurementsFairValueHierarchyDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_11ce0caf-9751-45ed-9879-df5c6eec101c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_99f3f3dc-b337-4307-968c-7a076247a1a9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_11ce0caf-9751-45ed-9879-df5c6eec101c" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_99f3f3dc-b337-4307-968c-7a076247a1a9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_8c251298-891a-4a79-8633-ab70c525e909" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_11ce0caf-9751-45ed-9879-df5c6eec101c" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_8c251298-891a-4a79-8633-ab70c525e909" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsFairValueDisclosure_e296f260-3c4d-4304-8d06-21396f3d307e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherAssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_11ce0caf-9751-45ed-9879-df5c6eec101c" xlink:to="loc_us-gaap_OtherAssetsFairValueDisclosure_e296f260-3c4d-4304-8d06-21396f3d307e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_151d2c7d-6fc7-479b-bb15-b307de5a8360" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_11ce0caf-9751-45ed-9879-df5c6eec101c" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_151d2c7d-6fc7-479b-bb15-b307de5a8360" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_311991a5-3585-427f-8ef9-6e4c3e2fcb16" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_11ce0caf-9751-45ed-9879-df5c6eec101c" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_311991a5-3585-427f-8ef9-6e4c3e2fcb16" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_f7dcefef-7d32-4d21-9903-2345d2ce3f67" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_311991a5-3585-427f-8ef9-6e4c3e2fcb16" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_f7dcefef-7d32-4d21-9903-2345d2ce3f67" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_f7dcefef-7d32-4d21-9903-2345d2ce3f67_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_f7dcefef-7d32-4d21-9903-2345d2ce3f67" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_f7dcefef-7d32-4d21-9903-2345d2ce3f67_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_27d51508-7c41-4005-a09d-abb8ef1520f2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_f7dcefef-7d32-4d21-9903-2345d2ce3f67" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_27d51508-7c41-4005-a09d-abb8ef1520f2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_01e4deb3-c3ca-4911-a1bb-2daaa0d2d9be" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_27d51508-7c41-4005-a09d-abb8ef1520f2" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_01e4deb3-c3ca-4911-a1bb-2daaa0d2d9be" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_f6bf1757-b750-49c3-9622-49d6098c4a04" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_27d51508-7c41-4005-a09d-abb8ef1520f2" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_f6bf1757-b750-49c3-9622-49d6098c4a04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_a5053e40-9797-427c-849f-6d093bd9abda" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_27d51508-7c41-4005-a09d-abb8ef1520f2" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_a5053e40-9797-427c-849f-6d093bd9abda" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_44e1beaa-a3e6-4728-a354-d056a3b314c6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_311991a5-3585-427f-8ef9-6e4c3e2fcb16" xlink:to="loc_us-gaap_FinancialInstrumentAxis_44e1beaa-a3e6-4728-a354-d056a3b314c6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_44e1beaa-a3e6-4728-a354-d056a3b314c6_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_44e1beaa-a3e6-4728-a354-d056a3b314c6" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_44e1beaa-a3e6-4728-a354-d056a3b314c6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_58d7cc14-0743-40c0-bfa8-54b9a559fd35" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_44e1beaa-a3e6-4728-a354-d056a3b314c6" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_58d7cc14-0743-40c0-bfa8-54b9a559fd35" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_cbf634b5-81c5-488b-b0c2-64c551538595" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_58d7cc14-0743-40c0-bfa8-54b9a559fd35" xlink:to="loc_us-gaap_MoneyMarketFundsMember_cbf634b5-81c5-488b-b0c2-64c551538595" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember_ec231288-038e-48ef-af9d-a092821e2735" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_58d7cc14-0743-40c0-bfa8-54b9a559fd35" xlink:to="loc_us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember_ec231288-038e-48ef-af9d-a092821e2735" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryAndGovernmentMember_988d5c40-629f-4669-9abb-3dd1389340d6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_USTreasuryAndGovernmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_58d7cc14-0743-40c0-bfa8-54b9a559fd35" xlink:to="loc_us-gaap_USTreasuryAndGovernmentMember_988d5c40-629f-4669-9abb-3dd1389340d6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_cce8df64-ecf6-451a-96f5-0af22033a8a5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_58d7cc14-0743-40c0-bfa8-54b9a559fd35" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_cce8df64-ecf6-451a-96f5-0af22033a8a5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CertificatesOfDepositMember_920e035f-28ed-43de-a640-ae3c3fe3724a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CertificatesOfDepositMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_58d7cc14-0743-40c0-bfa8-54b9a559fd35" xlink:to="loc_us-gaap_CertificatesOfDepositMember_920e035f-28ed-43de-a640-ae3c3fe3724a" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/FairValueMeasurementsRedeemableConvertiblePreferredStockWarrantLiabilityDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#FairValueMeasurementsRedeemableConvertiblePreferredStockWarrantLiabilityDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/FairValueMeasurementsRedeemableConvertiblePreferredStockWarrantLiabilityDetails" xlink:type="extended" id="ib133257699104c359b0132ea1946d47b_FairValueMeasurementsRedeemableConvertiblePreferredStockWarrantLiabilityDetails"/>
  <link:roleRef roleURI="http://asana.com/role/FairValueMeasurementsInvestmentsDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#FairValueMeasurementsInvestmentsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/FairValueMeasurementsInvestmentsDetails" xlink:type="extended" id="i752984d3b4a44b9ab269cd80425c46fa_FairValueMeasurementsInvestmentsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6324a5ae-6d6d-4668-aa62-00a2496d9ef5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrentAbstract_40449f64-02ab-4e16-bc67-9dfd473e7c4c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AssetsCurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6324a5ae-6d6d-4668-aa62-00a2496d9ef5" xlink:to="loc_us-gaap_AssetsCurrentAbstract_40449f64-02ab-4e16-bc67-9dfd473e7c4c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_4f4d0869-f117-42a1-8a86-aaf84c6e1cf1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_40449f64-02ab-4e16-bc67-9dfd473e7c4c" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_4f4d0869-f117-42a1-8a86-aaf84c6e1cf1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_b7114e3c-0fee-40f4-987d-da64526d9622" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_40449f64-02ab-4e16-bc67-9dfd473e7c4c" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_b7114e3c-0fee-40f4-987d-da64526d9622" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_79d1d036-3704-4e37-b19b-53b47bcb9f3c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_40449f64-02ab-4e16-bc67-9dfd473e7c4c" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_79d1d036-3704-4e37-b19b-53b47bcb9f3c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_01e6a278-7c4a-4c26-b271-99bc930c41ff" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_40449f64-02ab-4e16-bc67-9dfd473e7c4c" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_01e6a278-7c4a-4c26-b271-99bc930c41ff" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermInvestmentsAbstract_cb7fb791-8923-4dd2-9f0a-84bcab659067" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermInvestmentsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6324a5ae-6d6d-4668-aa62-00a2496d9ef5" xlink:to="loc_us-gaap_LongTermInvestmentsAbstract_cb7fb791-8923-4dd2-9f0a-84bcab659067" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecurities_1e1ae745-504f-41e3-a9ef-e36eb4c5ad72" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HeldToMaturitySecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_cb7fb791-8923-4dd2-9f0a-84bcab659067" xlink:to="loc_us-gaap_HeldToMaturitySecurities_1e1ae745-504f-41e3-a9ef-e36eb4c5ad72" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain_d0f727a0-5283-4f8f-acb1-922278f3d535" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_cb7fb791-8923-4dd2-9f0a-84bcab659067" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain_d0f727a0-5283-4f8f-acb1-922278f3d535" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss_e9db803b-62b2-436d-8599-1d83fbc53593" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_cb7fb791-8923-4dd2-9f0a-84bcab659067" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss_e9db803b-62b2-436d-8599-1d83fbc53593" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesFairValue_302683b1-1d2e-4c17-88d0-401e67d3cfc5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HeldToMaturitySecuritiesFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_cb7fb791-8923-4dd2-9f0a-84bcab659067" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesFairValue_302683b1-1d2e-4c17-88d0-401e67d3cfc5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecurities_7613f82b-2a17-42cc-8a53-0fe25e148d1a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6324a5ae-6d6d-4668-aa62-00a2496d9ef5" xlink:to="loc_us-gaap_DebtSecurities_7613f82b-2a17-42cc-8a53-0fe25e148d1a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_DebtSecuritiesAccumulatedUnrealizedGains_7a33ec4d-45fa-45ae-85e5-2bf891759b61" xlink:href="asan-20210131.xsd#asan_DebtSecuritiesAccumulatedUnrealizedGains"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6324a5ae-6d6d-4668-aa62-00a2496d9ef5" xlink:to="loc_asan_DebtSecuritiesAccumulatedUnrealizedGains_7a33ec4d-45fa-45ae-85e5-2bf891759b61" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_DebtSecuritiesAccumulatedUnrealizedLoss_61523458-f347-40a0-949a-5d1c6c585e85" xlink:href="asan-20210131.xsd#asan_DebtSecuritiesAccumulatedUnrealizedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6324a5ae-6d6d-4668-aa62-00a2496d9ef5" xlink:to="loc_asan_DebtSecuritiesAccumulatedUnrealizedLoss_61523458-f347-40a0-949a-5d1c6c585e85" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_DebtSecuritiesFairValue_9f54ff43-1c6e-4e17-b2a7-690b06dd5d2d" xlink:href="asan-20210131.xsd#asan_DebtSecuritiesFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6324a5ae-6d6d-4668-aa62-00a2496d9ef5" xlink:to="loc_asan_DebtSecuritiesFairValue_9f54ff43-1c6e-4e17-b2a7-690b06dd5d2d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_24f7d6aa-4c9c-4373-9078-1e2f6a01bba2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6324a5ae-6d6d-4668-aa62-00a2496d9ef5" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_24f7d6aa-4c9c-4373-9078-1e2f6a01bba2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_727757f0-678f-422f-b7d2-ae5827d79a80" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_24f7d6aa-4c9c-4373-9078-1e2f6a01bba2" xlink:to="loc_us-gaap_FinancialInstrumentAxis_727757f0-678f-422f-b7d2-ae5827d79a80" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_727757f0-678f-422f-b7d2-ae5827d79a80_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_727757f0-678f-422f-b7d2-ae5827d79a80" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_727757f0-678f-422f-b7d2-ae5827d79a80_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c0333064-dba3-453c-ac80-bd2004579f15" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_727757f0-678f-422f-b7d2-ae5827d79a80" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c0333064-dba3-453c-ac80-bd2004579f15" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember_2a7a940d-468c-466c-9372-2289a3e43a21" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c0333064-dba3-453c-ac80-bd2004579f15" xlink:to="loc_us-gaap_CommercialPaperNotIncludedWithCashAndCashEquivalentsMember_2a7a940d-468c-466c-9372-2289a3e43a21" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryAndGovernmentMember_0db3dddb-515e-4200-875c-ff99a97df557" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_USTreasuryAndGovernmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c0333064-dba3-453c-ac80-bd2004579f15" xlink:to="loc_us-gaap_USTreasuryAndGovernmentMember_0db3dddb-515e-4200-875c-ff99a97df557" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_7c814854-9942-4929-b036-5c29e040764f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c0333064-dba3-453c-ac80-bd2004579f15" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_7c814854-9942-4929-b036-5c29e040764f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CertificatesOfDepositMember_6b1c3b8e-b9fc-4da0-91ae-88aba42beec5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CertificatesOfDepositMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c0333064-dba3-453c-ac80-bd2004579f15" xlink:to="loc_us-gaap_CertificatesOfDepositMember_6b1c3b8e-b9fc-4da0-91ae-88aba42beec5" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#FairValueMeasurementsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="extended" id="id07a3364c6c643b18952eafbff095b27_FairValueMeasurementsNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_a3a8796e-63d8-47ea-8926-1e3f5754f691" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtFairValueDisclosures_9521f4b0-730f-46f4-b824-00feb7234395" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConvertibleDebtFairValueDisclosures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_a3a8796e-63d8-47ea-8926-1e3f5754f691" xlink:to="loc_us-gaap_ConvertibleDebtFairValueDisclosures_9521f4b0-730f-46f4-b824-00feb7234395" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_ef03c165-b964-4fcd-b0cf-0fe785566df2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_a3a8796e-63d8-47ea-8926-1e3f5754f691" xlink:to="loc_us-gaap_DebtInstrumentTable_ef03c165-b964-4fcd-b0cf-0fe785566df2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_436f6c3e-b57b-4df7-bc1e-6a19056e0de4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_ef03c165-b964-4fcd-b0cf-0fe785566df2" xlink:to="loc_us-gaap_DebtInstrumentAxis_436f6c3e-b57b-4df7-bc1e-6a19056e0de4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_436f6c3e-b57b-4df7-bc1e-6a19056e0de4_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_436f6c3e-b57b-4df7-bc1e-6a19056e0de4" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_436f6c3e-b57b-4df7-bc1e-6a19056e0de4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_356889a6-1b57-43d2-ab5f-a60b17074307" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_436f6c3e-b57b-4df7-bc1e-6a19056e0de4" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_356889a6-1b57-43d2-ab5f-a60b17074307" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_January2020ConvertibleNoteMember_1a6534ea-953a-4985-b015-ce7e00ea6b3f" xlink:href="asan-20210131.xsd#asan_January2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_356889a6-1b57-43d2-ab5f-a60b17074307" xlink:to="loc_asan_January2020ConvertibleNoteMember_1a6534ea-953a-4985-b015-ce7e00ea6b3f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_June2020ConvertibleNoteMember_2acc63c4-b032-4920-a5ed-7b71dce198fc" xlink:href="asan-20210131.xsd#asan_June2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_356889a6-1b57-43d2-ab5f-a60b17074307" xlink:to="loc_asan_June2020ConvertibleNoteMember_2acc63c4-b032-4920-a5ed-7b71dce198fc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_b1202c4d-eab8-4dad-97ca-3e867aaafd36" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_ef03c165-b964-4fcd-b0cf-0fe785566df2" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_b1202c4d-eab8-4dad-97ca-3e867aaafd36" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_b1202c4d-eab8-4dad-97ca-3e867aaafd36_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_b1202c4d-eab8-4dad-97ca-3e867aaafd36" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_b1202c4d-eab8-4dad-97ca-3e867aaafd36_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_b48ecbf8-dfe9-4a81-82db-3cb3d5486625" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_b1202c4d-eab8-4dad-97ca-3e867aaafd36" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_b48ecbf8-dfe9-4a81-82db-3cb3d5486625" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_78206f17-57f6-4f9d-84e7-4a2e37942bc0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_b48ecbf8-dfe9-4a81-82db-3cb3d5486625" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_78206f17-57f6-4f9d-84e7-4a2e37942bc0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_fc6d95ff-4e9b-4801-994d-86bba31fb2e2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_b48ecbf8-dfe9-4a81-82db-3cb3d5486625" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_fc6d95ff-4e9b-4801-994d-86bba31fb2e2" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/BalanceSheetComponents" xlink:type="simple" xlink:href="asan-20210131.xsd#BalanceSheetComponents"/>
  <link:definitionLink xlink:role="http://asana.com/role/BalanceSheetComponents" xlink:type="extended" id="ia0a5349c61a44ed3a956a90c7aa55b54_BalanceSheetComponents"/>
  <link:roleRef roleURI="http://asana.com/role/BalanceSheetComponentsTables" xlink:type="simple" xlink:href="asan-20210131.xsd#BalanceSheetComponentsTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/BalanceSheetComponentsTables" xlink:type="extended" id="i6d15e0d2fcd94bd1926dd733d8f116d7_BalanceSheetComponentsTables"/>
  <link:roleRef roleURI="http://asana.com/role/BalanceSheetComponentsPropertyandEquipmentDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#BalanceSheetComponentsPropertyandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BalanceSheetComponentsPropertyandEquipmentDetails" xlink:type="extended" id="i6c75e7e0d21546e2b72ccbf30e6ee4cb_BalanceSheetComponentsPropertyandEquipmentDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_cdb9b7a9-baed-4521-b2fc-bf010ba8369b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_e4ff3faf-dd2d-4d3f-bcdc-90e625bc625e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_cdb9b7a9-baed-4521-b2fc-bf010ba8369b" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_e4ff3faf-dd2d-4d3f-bcdc-90e625bc625e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_5ce8bea8-560e-4837-99e5-485bf2c76c1b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_cdb9b7a9-baed-4521-b2fc-bf010ba8369b" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_5ce8bea8-560e-4837-99e5-485bf2c76c1b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_7ed6ff83-5d3f-4656-97f9-a5875b153e24" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_cdb9b7a9-baed-4521-b2fc-bf010ba8369b" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_7ed6ff83-5d3f-4656-97f9-a5875b153e24" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_7824a378-6797-4a19-bc6c-c4baf0b639c6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_cdb9b7a9-baed-4521-b2fc-bf010ba8369b" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_7824a378-6797-4a19-bc6c-c4baf0b639c6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_e3513887-0712-4dfd-932a-194a6e78b605" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_cdb9b7a9-baed-4521-b2fc-bf010ba8369b" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_e3513887-0712-4dfd-932a-194a6e78b605" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_713b76f1-d5ea-4099-9de5-21d471f96008" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_e3513887-0712-4dfd-932a-194a6e78b605" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_713b76f1-d5ea-4099-9de5-21d471f96008" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_713b76f1-d5ea-4099-9de5-21d471f96008_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_713b76f1-d5ea-4099-9de5-21d471f96008" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_713b76f1-d5ea-4099-9de5-21d471f96008_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_2a41aaf8-ae95-4eba-bf1e-5c578d1c5305" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_713b76f1-d5ea-4099-9de5-21d471f96008" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_2a41aaf8-ae95-4eba-bf1e-5c578d1c5305" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerEquipmentMember_112aa8db-caa8-4adb-8eb0-325390d24bb3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_2a41aaf8-ae95-4eba-bf1e-5c578d1c5305" xlink:to="loc_us-gaap_ComputerEquipmentMember_112aa8db-caa8-4adb-8eb0-325390d24bb3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_c827b234-75aa-404b-b68f-35ee55de2929" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FurnitureAndFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_2a41aaf8-ae95-4eba-bf1e-5c578d1c5305" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_c827b234-75aa-404b-b68f-35ee55de2929" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_33782d46-6337-46fd-8d2b-d2650e1d331e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_2a41aaf8-ae95-4eba-bf1e-5c578d1c5305" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_33782d46-6337-46fd-8d2b-d2650e1d331e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SoftwareAndSoftwareDevelopmentCostsMember_c9dcaa84-afeb-4de0-8f73-99c7fc4d2118" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SoftwareAndSoftwareDevelopmentCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_2a41aaf8-ae95-4eba-bf1e-5c578d1c5305" xlink:to="loc_us-gaap_SoftwareAndSoftwareDevelopmentCostsMember_c9dcaa84-afeb-4de0-8f73-99c7fc4d2118" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConstructionInProgressMember_964f5d4c-7ba7-4ac1-be7c-3598902bcd75" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConstructionInProgressMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_2a41aaf8-ae95-4eba-bf1e-5c578d1c5305" xlink:to="loc_us-gaap_ConstructionInProgressMember_964f5d4c-7ba7-4ac1-be7c-3598902bcd75" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/BalanceSheetComponentsCapitalizedSoftwareCostsDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#BalanceSheetComponentsCapitalizedSoftwareCostsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BalanceSheetComponentsCapitalizedSoftwareCostsDetails" xlink:type="extended" id="ifcf90465c055426fa8575a7d0d9e45c6_BalanceSheetComponentsCapitalizedSoftwareCostsDetails"/>
  <link:roleRef roleURI="http://asana.com/role/BalanceSheetComponentsPrepaidExpensesandOtherCurrentAssetsDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#BalanceSheetComponentsPrepaidExpensesandOtherCurrentAssetsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BalanceSheetComponentsPrepaidExpensesandOtherCurrentAssetsDetails" xlink:type="extended" id="i4663a9d393df4c6c86d8a3e5edd6961c_BalanceSheetComponentsPrepaidExpensesandOtherCurrentAssetsDetails"/>
  <link:roleRef roleURI="http://asana.com/role/BalanceSheetComponentsAccruedExpensesandOtherCurrentLiabilitiesDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#BalanceSheetComponentsAccruedExpensesandOtherCurrentLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/BalanceSheetComponentsAccruedExpensesandOtherCurrentLiabilitiesDetails" xlink:type="extended" id="id9f3d498457446a8b3a104047d9d2a35_BalanceSheetComponentsAccruedExpensesandOtherCurrentLiabilitiesDetails"/>
  <link:roleRef roleURI="http://asana.com/role/ConvertibleNotesRelatedParty" xlink:type="simple" xlink:href="asan-20210131.xsd#ConvertibleNotesRelatedParty"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConvertibleNotesRelatedParty" xlink:type="extended" id="i0b8630b3541e428ca0e8d1bedb29a6ad_ConvertibleNotesRelatedParty"/>
  <link:roleRef roleURI="http://asana.com/role/ConvertibleNotesRelatedPartyTables" xlink:type="simple" xlink:href="asan-20210131.xsd#ConvertibleNotesRelatedPartyTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConvertibleNotesRelatedPartyTables" xlink:type="extended" id="iad6c027296284be5ad350cba165466bf_ConvertibleNotesRelatedPartyTables"/>
  <link:roleRef roleURI="http://asana.com/role/ConvertibleNotesRelatedPartyNarrativeDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#ConvertibleNotesRelatedPartyNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConvertibleNotesRelatedPartyNarrativeDetails" xlink:type="extended" id="i051c4f3c923948318bc6fa9197b777a3_ConvertibleNotesRelatedPartyNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_a099d4a5-0129-4b48-926e-53a62d847e1d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_ea62578f-5e0d-4510-9522-91d6a032ebc6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_a099d4a5-0129-4b48-926e-53a62d847e1d" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_ea62578f-5e0d-4510-9522-91d6a032ebc6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_cd10cfbe-f17a-4b30-b4b6-33593964e364" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_a099d4a5-0129-4b48-926e-53a62d847e1d" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_cd10cfbe-f17a-4b30-b4b6-33593964e364" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_b2162df1-1d2b-41b3-b1be-1b83a99d100b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_a099d4a5-0129-4b48-926e-53a62d847e1d" xlink:to="loc_us-gaap_LongTermDebt_b2162df1-1d2b-41b3-b1be-1b83a99d100b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtConversionConvertedInstrumentSharesIssued1_fe3c7839-a873-4939-a971-4badaf305080" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtConversionConvertedInstrumentSharesIssued1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_a099d4a5-0129-4b48-926e-53a62d847e1d" xlink:to="loc_us-gaap_DebtConversionConvertedInstrumentSharesIssued1_fe3c7839-a873-4939-a971-4badaf305080" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentConvertibleConversionPrice1_3613e562-19fd-4b7a-a29c-eaf4ea9653cb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentConvertibleConversionPrice1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_a099d4a5-0129-4b48-926e-53a62d847e1d" xlink:to="loc_us-gaap_DebtInstrumentConvertibleConversionPrice1_3613e562-19fd-4b7a-a29c-eaf4ea9653cb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_d2fb1e2e-e3f4-44a8-9bac-4a7401af1db4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_a099d4a5-0129-4b48-926e-53a62d847e1d" xlink:to="loc_us-gaap_AdditionalPaidInCapital_d2fb1e2e-e3f4-44a8-9bac-4a7401af1db4" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalEquityComponentOfConvertibleDebt_0b21826a-4196-47c7-8e14-cd196f780d47" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalEquityComponentOfConvertibleDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_a099d4a5-0129-4b48-926e-53a62d847e1d" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalEquityComponentOfConvertibleDebt_0b21826a-4196-47c7-8e14-cd196f780d47" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_e3eaa3a2-6821-40a7-8a60-d425aa1bd6f3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_a099d4a5-0129-4b48-926e-53a62d847e1d" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_e3eaa3a2-6821-40a7-8a60-d425aa1bd6f3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_a1c20b98-b6f5-43dd-b329-1352570ee409" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_e3eaa3a2-6821-40a7-8a60-d425aa1bd6f3" xlink:to="loc_us-gaap_DebtInstrumentAxis_a1c20b98-b6f5-43dd-b329-1352570ee409" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_a1c20b98-b6f5-43dd-b329-1352570ee409_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_a1c20b98-b6f5-43dd-b329-1352570ee409" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_a1c20b98-b6f5-43dd-b329-1352570ee409_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_9fa4c51a-fd11-463e-96fa-448fe7f623b2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_a1c20b98-b6f5-43dd-b329-1352570ee409" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_9fa4c51a-fd11-463e-96fa-448fe7f623b2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_January2020ConvertibleNoteMember_f7e120bd-c37c-4b0f-9cfe-a9997102fe84" xlink:href="asan-20210131.xsd#asan_January2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9fa4c51a-fd11-463e-96fa-448fe7f623b2" xlink:to="loc_asan_January2020ConvertibleNoteMember_f7e120bd-c37c-4b0f-9cfe-a9997102fe84" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_June2020ConvertibleNoteMember_1ec40a6a-f87a-4b78-81d9-e625183b9b1f" xlink:href="asan-20210131.xsd#asan_June2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9fa4c51a-fd11-463e-96fa-448fe7f623b2" xlink:to="loc_asan_June2020ConvertibleNoteMember_1ec40a6a-f87a-4b78-81d9-e625183b9b1f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_January2020ConvertibleNoteSettlementAmountMember_8c9940c0-c823-41d4-af5d-f2a7d76f0025" xlink:href="asan-20210131.xsd#asan_January2020ConvertibleNoteSettlementAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9fa4c51a-fd11-463e-96fa-448fe7f623b2" xlink:to="loc_asan_January2020ConvertibleNoteSettlementAmountMember_8c9940c0-c823-41d4-af5d-f2a7d76f0025" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_TermLoanAgreementMember_6da212eb-6fca-43c1-a633-2a411efeb86c" xlink:href="asan-20210131.xsd#asan_TermLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9fa4c51a-fd11-463e-96fa-448fe7f623b2" xlink:to="loc_asan_TermLoanAgreementMember_6da212eb-6fca-43c1-a633-2a411efeb86c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_June2020ConvertibleNoteSettlementAmountMember_525759a4-c821-4ebc-ad82-54a6ddc4d928" xlink:href="asan-20210131.xsd#asan_June2020ConvertibleNoteSettlementAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9fa4c51a-fd11-463e-96fa-448fe7f623b2" xlink:to="loc_asan_June2020ConvertibleNoteSettlementAmountMember_525759a4-c821-4ebc-ad82-54a6ddc4d928" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_656c8081-df26-4510-b357-d769f209e1b9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_e3eaa3a2-6821-40a7-8a60-d425aa1bd6f3" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_656c8081-df26-4510-b357-d769f209e1b9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_656c8081-df26-4510-b357-d769f209e1b9_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_656c8081-df26-4510-b357-d769f209e1b9" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_656c8081-df26-4510-b357-d769f209e1b9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_08c04552-b891-46f7-b6de-d0ba289ed0f2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_656c8081-df26-4510-b357-d769f209e1b9" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_08c04552-b891-46f7-b6de-d0ba289ed0f2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtMember_87644b94-b9f8-4843-b0e8-7a67816acb94" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConvertibleDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_08c04552-b891-46f7-b6de-d0ba289ed0f2" xlink:to="loc_us-gaap_ConvertibleDebtMember_87644b94-b9f8-4843-b0e8-7a67816acb94" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_9d23e282-8ad4-451b-8c46-e5da68d1b1b6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_08c04552-b891-46f7-b6de-d0ba289ed0f2" xlink:to="loc_us-gaap_SecuredDebtMember_9d23e282-8ad4-451b-8c46-e5da68d1b1b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_e63282e4-38a6-4dd5-b529-1cb5a5118560" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_e3eaa3a2-6821-40a7-8a60-d425aa1bd6f3" xlink:to="loc_srt_RangeAxis_e63282e4-38a6-4dd5-b529-1cb5a5118560" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_e63282e4-38a6-4dd5-b529-1cb5a5118560_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_e63282e4-38a6-4dd5-b529-1cb5a5118560" xlink:to="loc_srt_RangeMember_e63282e4-38a6-4dd5-b529-1cb5a5118560_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_db64f4b6-501b-4b22-918f-82bcab8e41cd" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_e63282e4-38a6-4dd5-b529-1cb5a5118560" xlink:to="loc_srt_RangeMember_db64f4b6-501b-4b22-918f-82bcab8e41cd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_f95377f3-0e72-459d-8ffb-60f4808a9ad6" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_db64f4b6-501b-4b22-918f-82bcab8e41cd" xlink:to="loc_srt_MaximumMember_f95377f3-0e72-459d-8ffb-60f4808a9ad6" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/ConvertibleNotesRelatedPartyNetCarryingAmountofConvertibleNotesDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#ConvertibleNotesRelatedPartyNetCarryingAmountofConvertibleNotesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConvertibleNotesRelatedPartyNetCarryingAmountofConvertibleNotesDetails" xlink:type="extended" id="ie8957a4deb7f4f81b24f1fa3cb24f973_ConvertibleNotesRelatedPartyNetCarryingAmountofConvertibleNotesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_b8df026d-8b34-4c33-b622-d45b1087470b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_ea0fe579-b599-40fc-88a6-9df3f7c625b1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b8df026d-8b34-4c33-b622-d45b1087470b" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_ea0fe579-b599-40fc-88a6-9df3f7c625b1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnamortizedDiscount_c48ff16e-c93a-4483-aee0-26dd191d84ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentUnamortizedDiscount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b8df026d-8b34-4c33-b622-d45b1087470b" xlink:to="loc_us-gaap_DebtInstrumentUnamortizedDiscount_c48ff16e-c93a-4483-aee0-26dd191d84ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestPayableCurrentAndNoncurrent_637088ea-6a3b-46f0-a33b-66b29482ae91" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InterestPayableCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b8df026d-8b34-4c33-b622-d45b1087470b" xlink:to="loc_us-gaap_InterestPayableCurrentAndNoncurrent_637088ea-6a3b-46f0-a33b-66b29482ae91" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_57bc7fe2-ba40-4c17-9246-936a88038941" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b8df026d-8b34-4c33-b622-d45b1087470b" xlink:to="loc_us-gaap_LongTermDebt_57bc7fe2-ba40-4c17-9246-936a88038941" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_DebtConversionPotentiallyIssuableSecurities_d322a495-8d1e-4519-b915-1bf9217ef46b" xlink:href="asan-20210131.xsd#asan_DebtConversionPotentiallyIssuableSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b8df026d-8b34-4c33-b622-d45b1087470b" xlink:to="loc_asan_DebtConversionPotentiallyIssuableSecurities_d322a495-8d1e-4519-b915-1bf9217ef46b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentConvertibleConversionPrice1_6921b307-fc1e-40b9-adc3-0e118bb40a32" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentConvertibleConversionPrice1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b8df026d-8b34-4c33-b622-d45b1087470b" xlink:to="loc_us-gaap_DebtInstrumentConvertibleConversionPrice1_6921b307-fc1e-40b9-adc3-0e118bb40a32" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_e30919e9-1696-457a-af13-4756bf9e3853" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b8df026d-8b34-4c33-b622-d45b1087470b" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_e30919e9-1696-457a-af13-4756bf9e3853" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_862d8c84-9838-42cc-81ee-82ef959ce823" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_e30919e9-1696-457a-af13-4756bf9e3853" xlink:to="loc_us-gaap_DebtInstrumentAxis_862d8c84-9838-42cc-81ee-82ef959ce823" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_862d8c84-9838-42cc-81ee-82ef959ce823_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_862d8c84-9838-42cc-81ee-82ef959ce823" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_862d8c84-9838-42cc-81ee-82ef959ce823_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_8bd43378-aa40-4a80-b74f-0cc6101183fa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_862d8c84-9838-42cc-81ee-82ef959ce823" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_8bd43378-aa40-4a80-b74f-0cc6101183fa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_January2020ConvertibleNoteMember_9b671b96-58cb-41d3-a121-59a5ea52bf5d" xlink:href="asan-20210131.xsd#asan_January2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_8bd43378-aa40-4a80-b74f-0cc6101183fa" xlink:to="loc_asan_January2020ConvertibleNoteMember_9b671b96-58cb-41d3-a121-59a5ea52bf5d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_June2020ConvertibleNoteMember_fa87ca5e-0945-40d5-932f-b7305f57a1d3" xlink:href="asan-20210131.xsd#asan_June2020ConvertibleNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_8bd43378-aa40-4a80-b74f-0cc6101183fa" xlink:to="loc_asan_June2020ConvertibleNoteMember_fa87ca5e-0945-40d5-932f-b7305f57a1d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_e951f6ed-a046-4ed7-896f-fdfec82e80fd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_e30919e9-1696-457a-af13-4756bf9e3853" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_e951f6ed-a046-4ed7-896f-fdfec82e80fd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_e951f6ed-a046-4ed7-896f-fdfec82e80fd_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_e951f6ed-a046-4ed7-896f-fdfec82e80fd" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_e951f6ed-a046-4ed7-896f-fdfec82e80fd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_5b83a50b-1351-41a6-8d8b-4d8c5ba038a3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_e951f6ed-a046-4ed7-896f-fdfec82e80fd" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_5b83a50b-1351-41a6-8d8b-4d8c5ba038a3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtMember_11c7e6e8-e335-4eeb-a70d-562d4ce4c514" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConvertibleDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_5b83a50b-1351-41a6-8d8b-4d8c5ba038a3" xlink:to="loc_us-gaap_ConvertibleDebtMember_11c7e6e8-e335-4eeb-a70d-562d4ce4c514" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_388b1c85-b9df-4959-8097-0303c50b0193" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_e30919e9-1696-457a-af13-4756bf9e3853" xlink:to="loc_srt_RangeAxis_388b1c85-b9df-4959-8097-0303c50b0193" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_388b1c85-b9df-4959-8097-0303c50b0193_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_388b1c85-b9df-4959-8097-0303c50b0193" xlink:to="loc_srt_RangeMember_388b1c85-b9df-4959-8097-0303c50b0193_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_f80c277a-2cfd-4e39-97cc-c39edcdc9e42" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_388b1c85-b9df-4959-8097-0303c50b0193" xlink:to="loc_srt_RangeMember_f80c277a-2cfd-4e39-97cc-c39edcdc9e42" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_2bb9ca3b-8ae2-4102-9964-e2d5fbfc9fcd" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_f80c277a-2cfd-4e39-97cc-c39edcdc9e42" xlink:to="loc_srt_MinimumMember_2bb9ca3b-8ae2-4102-9964-e2d5fbfc9fcd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_afe9e8d9-0b36-4179-bf23-c72e6e8dfb79" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_f80c277a-2cfd-4e39-97cc-c39edcdc9e42" xlink:to="loc_srt_MaximumMember_afe9e8d9-0b36-4179-bf23-c72e6e8dfb79" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/ConvertibleNotesRelatedPartyInterestExpenseDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#ConvertibleNotesRelatedPartyInterestExpenseDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/ConvertibleNotesRelatedPartyInterestExpenseDetails" xlink:type="extended" id="i4821c5dbf7774c5c85b7ed599fbc1c23_ConvertibleNotesRelatedPartyInterestExpenseDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_dd25cfad-1cc1-4252-84bb-20d54e059669" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfDebtDiscountPremium_66efb62c-9b66-4ec4-8f61-826d2266a30e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AmortizationOfDebtDiscountPremium"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_dd25cfad-1cc1-4252-84bb-20d54e059669" xlink:to="loc_us-gaap_AmortizationOfDebtDiscountPremium_66efb62c-9b66-4ec4-8f61-826d2266a30e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDebtExcludingAmortization_b0006c80-a440-4f04-8c1a-c4e2a2852663" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InterestExpenseDebtExcludingAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_dd25cfad-1cc1-4252-84bb-20d54e059669" xlink:to="loc_us-gaap_InterestExpenseDebtExcludingAmortization_b0006c80-a440-4f04-8c1a-c4e2a2852663" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDebt_d0c2641c-b83a-426d-914b-b9d13e16504f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InterestExpenseDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_dd25cfad-1cc1-4252-84bb-20d54e059669" xlink:to="loc_us-gaap_InterestExpenseDebt_d0c2641c-b83a-426d-914b-b9d13e16504f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_81a9b0b0-d7cb-4dde-9a14-7f459315542b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_dd25cfad-1cc1-4252-84bb-20d54e059669" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_81a9b0b0-d7cb-4dde-9a14-7f459315542b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_44843048-5966-4169-8532-7b8632d19426" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_81a9b0b0-d7cb-4dde-9a14-7f459315542b" xlink:to="loc_us-gaap_DebtInstrumentAxis_44843048-5966-4169-8532-7b8632d19426" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_44843048-5966-4169-8532-7b8632d19426_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_44843048-5966-4169-8532-7b8632d19426" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_44843048-5966-4169-8532-7b8632d19426_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_f8f7bf50-5130-4e32-a21c-1b6873995c1b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_44843048-5966-4169-8532-7b8632d19426" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_f8f7bf50-5130-4e32-a21c-1b6873995c1b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleNotesPayableMember_44c19732-142c-4ac9-9f09-6510ee25ab1d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConvertibleNotesPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_f8f7bf50-5130-4e32-a21c-1b6873995c1b" xlink:to="loc_us-gaap_ConvertibleNotesPayableMember_44c19732-142c-4ac9-9f09-6510ee25ab1d" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/Debt" xlink:type="simple" xlink:href="asan-20210131.xsd#Debt"/>
  <link:definitionLink xlink:role="http://asana.com/role/Debt" xlink:type="extended" id="i4fe7b0e79e1e48da96a6e1e39be68869_Debt"/>
  <link:roleRef roleURI="http://asana.com/role/DebtTables" xlink:type="simple" xlink:href="asan-20210131.xsd#DebtTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/DebtTables" xlink:type="extended" id="iaf11506f541a41cdacc5a12c3d5a582a_DebtTables"/>
  <link:roleRef roleURI="http://asana.com/role/DebtNarrativeDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#DebtNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/DebtNarrativeDetails" xlink:type="extended" id="ie611f1b8a16e4548a73327514970415c_DebtNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_0372f615-346c-42da-ba91-607c54b99a6b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtTerm_d594ba59-bc58-4522-b595-8dc40dcb96d5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebtTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_0372f615-346c-42da-ba91-607c54b99a6b" xlink:to="loc_us-gaap_LongTermDebtTerm_d594ba59-bc58-4522-b595-8dc40dcb96d5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_1001d34e-077b-4141-be9f-d0586a80dbfc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_0372f615-346c-42da-ba91-607c54b99a6b" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_1001d34e-077b-4141-be9f-d0586a80dbfc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_88ec6bc5-d084-4cf1-9560-cf4fc4697eb2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_0372f615-346c-42da-ba91-607c54b99a6b" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_88ec6bc5-d084-4cf1-9560-cf4fc4697eb2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_DebtInstrumentCovenantTermsCashBalanceRequirementAmount_cbbb9ac4-36ee-4be0-b522-bd16edc440fc" xlink:href="asan-20210131.xsd#asan_DebtInstrumentCovenantTermsCashBalanceRequirementAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_0372f615-346c-42da-ba91-607c54b99a6b" xlink:to="loc_asan_DebtInstrumentCovenantTermsCashBalanceRequirementAmount_cbbb9ac4-36ee-4be0-b522-bd16edc440fc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_d01d700d-b980-4911-9768-1755a6135089" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_0372f615-346c-42da-ba91-607c54b99a6b" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_d01d700d-b980-4911-9768-1755a6135089" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_7a079313-c761-40a2-a773-b1b997632ec3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_0372f615-346c-42da-ba91-607c54b99a6b" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_7a079313-c761-40a2-a773-b1b997632ec3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_7f3b02f8-be9d-4502-8cc5-0dea9fe60171" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_7a079313-c761-40a2-a773-b1b997632ec3" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_7f3b02f8-be9d-4502-8cc5-0dea9fe60171" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_7f3b02f8-be9d-4502-8cc5-0dea9fe60171_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_7f3b02f8-be9d-4502-8cc5-0dea9fe60171" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_7f3b02f8-be9d-4502-8cc5-0dea9fe60171_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_a671cbdc-1742-4b90-bfcd-4987e790fc4e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_7f3b02f8-be9d-4502-8cc5-0dea9fe60171" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_a671cbdc-1742-4b90-bfcd-4987e790fc4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_8f855b9a-e4e0-4089-81c5-3f2824f1bc51" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_a671cbdc-1742-4b90-bfcd-4987e790fc4e" xlink:to="loc_us-gaap_SecuredDebtMember_8f855b9a-e4e0-4089-81c5-3f2824f1bc51" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_1fafdb99-16ea-432e-bdab-ab346547b44e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_7a079313-c761-40a2-a773-b1b997632ec3" xlink:to="loc_us-gaap_DebtInstrumentAxis_1fafdb99-16ea-432e-bdab-ab346547b44e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_1fafdb99-16ea-432e-bdab-ab346547b44e_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_1fafdb99-16ea-432e-bdab-ab346547b44e" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_1fafdb99-16ea-432e-bdab-ab346547b44e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_563a4a67-88c8-477a-9618-aaab8dddee70" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_1fafdb99-16ea-432e-bdab-ab346547b44e" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_563a4a67-88c8-477a-9618-aaab8dddee70" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_TermLoanAgreementMember_c12461d3-a8b1-4d69-9074-ad4f06cf4434" xlink:href="asan-20210131.xsd#asan_TermLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_563a4a67-88c8-477a-9618-aaab8dddee70" xlink:to="loc_asan_TermLoanAgreementMember_c12461d3-a8b1-4d69-9074-ad4f06cf4434" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_57936514-7141-46ec-b70b-a89d7b47c404" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_7a079313-c761-40a2-a773-b1b997632ec3" xlink:to="loc_srt_RangeAxis_57936514-7141-46ec-b70b-a89d7b47c404" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_57936514-7141-46ec-b70b-a89d7b47c404_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_57936514-7141-46ec-b70b-a89d7b47c404" xlink:to="loc_srt_RangeMember_57936514-7141-46ec-b70b-a89d7b47c404_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_8e0d7174-5075-4239-91f3-3b4c0f793067" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_57936514-7141-46ec-b70b-a89d7b47c404" xlink:to="loc_srt_RangeMember_8e0d7174-5075-4239-91f3-3b4c0f793067" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_54c43019-3a3a-4201-b66a-5c3e7cba1d97" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_8e0d7174-5075-4239-91f3-3b4c0f793067" xlink:to="loc_srt_MinimumMember_54c43019-3a3a-4201-b66a-5c3e7cba1d97" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_d3aaff6a-248f-41e8-8d6c-f15fb34adc87" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_8e0d7174-5075-4239-91f3-3b4c0f793067" xlink:to="loc_srt_MaximumMember_d3aaff6a-248f-41e8-8d6c-f15fb34adc87" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_2a32b176-94fe-46d2-9db0-a617ae5665ae" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_7a079313-c761-40a2-a773-b1b997632ec3" xlink:to="loc_us-gaap_VariableRateAxis_2a32b176-94fe-46d2-9db0-a617ae5665ae" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_2a32b176-94fe-46d2-9db0-a617ae5665ae_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_2a32b176-94fe-46d2-9db0-a617ae5665ae" xlink:to="loc_us-gaap_VariableRateDomain_2a32b176-94fe-46d2-9db0-a617ae5665ae_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_fe1e12a3-7d75-4748-acc6-ee31ee54fb88" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_2a32b176-94fe-46d2-9db0-a617ae5665ae" xlink:to="loc_us-gaap_VariableRateDomain_fe1e12a3-7d75-4748-acc6-ee31ee54fb88" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrimeRateMember_0e144acd-f9c6-4cbd-897b-36c31f42170d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PrimeRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_fe1e12a3-7d75-4748-acc6-ee31ee54fb88" xlink:to="loc_us-gaap_PrimeRateMember_0e144acd-f9c6-4cbd-897b-36c31f42170d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_9ffba428-7543-4a97-bd64-0fa550ccd97d" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_7a079313-c761-40a2-a773-b1b997632ec3" xlink:to="loc_srt_StatementScenarioAxis_9ffba428-7543-4a97-bd64-0fa550ccd97d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_9ffba428-7543-4a97-bd64-0fa550ccd97d_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_9ffba428-7543-4a97-bd64-0fa550ccd97d" xlink:to="loc_srt_ScenarioUnspecifiedDomain_9ffba428-7543-4a97-bd64-0fa550ccd97d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_0d3e2bc8-e125-4db2-985f-ec60e2680ec9" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_9ffba428-7543-4a97-bd64-0fa550ccd97d" xlink:to="loc_srt_ScenarioUnspecifiedDomain_0d3e2bc8-e125-4db2-985f-ec60e2680ec9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_UnrestrictedCashBalanceTierOneMember_e07ad6b1-2191-4d0a-a56d-8f8d75864136" xlink:href="asan-20210131.xsd#asan_UnrestrictedCashBalanceTierOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_0d3e2bc8-e125-4db2-985f-ec60e2680ec9" xlink:to="loc_asan_UnrestrictedCashBalanceTierOneMember_e07ad6b1-2191-4d0a-a56d-8f8d75864136" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_UnrestrictedCashBalanceTierTwoMember_0daf2fae-a022-42b0-b1f5-d0d747bf7196" xlink:href="asan-20210131.xsd#asan_UnrestrictedCashBalanceTierTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_0d3e2bc8-e125-4db2-985f-ec60e2680ec9" xlink:to="loc_asan_UnrestrictedCashBalanceTierTwoMember_0daf2fae-a022-42b0-b1f5-d0d747bf7196" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_UnrestrictedCashBalanceTierThreeMember_e6ff0af3-a27c-4304-b235-2a991de4282e" xlink:href="asan-20210131.xsd#asan_UnrestrictedCashBalanceTierThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_0d3e2bc8-e125-4db2-985f-ec60e2680ec9" xlink:to="loc_asan_UnrestrictedCashBalanceTierThreeMember_e6ff0af3-a27c-4304-b235-2a991de4282e" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/DebtNetCarryingAmountofTermLoanDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#DebtNetCarryingAmountofTermLoanDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/DebtNetCarryingAmountofTermLoanDetails" xlink:type="extended" id="i46f6778f661e434db4e93fab4a4721e8_DebtNetCarryingAmountofTermLoanDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_fe915939-274c-4727-96ea-7bec8c4dce5e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_1ab08693-ca00-4680-a200-cf86e5fca226" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fe915939-274c-4727-96ea-7bec8c4dce5e" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_1ab08693-ca00-4680-a200-cf86e5fca226" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestPayableCurrentAndNoncurrent_a0556fff-9e85-4b9e-a042-8b5036d60295" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InterestPayableCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fe915939-274c-4727-96ea-7bec8c4dce5e" xlink:to="loc_us-gaap_InterestPayableCurrentAndNoncurrent_a0556fff-9e85-4b9e-a042-8b5036d60295" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredFinanceCostsNet_f72bd157-2ca7-4962-9c6b-f31522b21595" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DeferredFinanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fe915939-274c-4727-96ea-7bec8c4dce5e" xlink:to="loc_us-gaap_DeferredFinanceCostsNet_f72bd157-2ca7-4962-9c6b-f31522b21595" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_d08bb815-7113-4b02-b132-3cd1d8dfcde0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fe915939-274c-4727-96ea-7bec8c4dce5e" xlink:to="loc_us-gaap_LongTermDebt_d08bb815-7113-4b02-b132-3cd1d8dfcde0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtCurrent_3a754d76-be47-4a8a-a71f-ff42c3552c77" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebtCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fe915939-274c-4727-96ea-7bec8c4dce5e" xlink:to="loc_us-gaap_LongTermDebtCurrent_3a754d76-be47-4a8a-a71f-ff42c3552c77" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtNoncurrent_abc739d2-66c3-4a4c-92e9-8319ab414c62" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebtNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fe915939-274c-4727-96ea-7bec8c4dce5e" xlink:to="loc_us-gaap_LongTermDebtNoncurrent_abc739d2-66c3-4a4c-92e9-8319ab414c62" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_72a1db3c-2fbc-4390-8b45-44639986f14f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fe915939-274c-4727-96ea-7bec8c4dce5e" xlink:to="loc_us-gaap_DebtInstrumentTable_72a1db3c-2fbc-4390-8b45-44639986f14f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_d8c1abcb-e9e7-4ea8-8e2e-7d9772ab40b9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_72a1db3c-2fbc-4390-8b45-44639986f14f" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_d8c1abcb-e9e7-4ea8-8e2e-7d9772ab40b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_d8c1abcb-e9e7-4ea8-8e2e-7d9772ab40b9_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_d8c1abcb-e9e7-4ea8-8e2e-7d9772ab40b9" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_d8c1abcb-e9e7-4ea8-8e2e-7d9772ab40b9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_20d3818d-6b9c-419c-a712-859a4af77e86" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_d8c1abcb-e9e7-4ea8-8e2e-7d9772ab40b9" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_20d3818d-6b9c-419c-a712-859a4af77e86" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_a6b19411-d0f9-4f82-8e7c-a2124fd94211" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_20d3818d-6b9c-419c-a712-859a4af77e86" xlink:to="loc_us-gaap_SecuredDebtMember_a6b19411-d0f9-4f82-8e7c-a2124fd94211" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_5c2276e1-1adb-4f79-b85c-34df67549b08" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_72a1db3c-2fbc-4390-8b45-44639986f14f" xlink:to="loc_us-gaap_DebtInstrumentAxis_5c2276e1-1adb-4f79-b85c-34df67549b08" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_5c2276e1-1adb-4f79-b85c-34df67549b08_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_5c2276e1-1adb-4f79-b85c-34df67549b08" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_5c2276e1-1adb-4f79-b85c-34df67549b08_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_23628edc-5807-4c19-8d95-8921be2bc354" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_5c2276e1-1adb-4f79-b85c-34df67549b08" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_23628edc-5807-4c19-8d95-8921be2bc354" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_TermLoanAgreementMember_3cfd31f4-03cb-44f2-b391-6137340701ea" xlink:href="asan-20210131.xsd#asan_TermLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_23628edc-5807-4c19-8d95-8921be2bc354" xlink:to="loc_asan_TermLoanAgreementMember_3cfd31f4-03cb-44f2-b391-6137340701ea" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/CommitmentsandContingencies" xlink:type="simple" xlink:href="asan-20210131.xsd#CommitmentsandContingencies"/>
  <link:definitionLink xlink:role="http://asana.com/role/CommitmentsandContingencies" xlink:type="extended" id="i1a4adfe8cf4b43d2ae02c92b7ab39b78_CommitmentsandContingencies"/>
  <link:roleRef roleURI="http://asana.com/role/CommitmentsandContingenciesDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#CommitmentsandContingenciesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/CommitmentsandContingenciesDetails" xlink:type="extended" id="i6f4b97973cde471280cd4a64fb2cbc53_CommitmentsandContingenciesDetails"/>
  <link:roleRef roleURI="http://asana.com/role/Leases" xlink:type="simple" xlink:href="asan-20210131.xsd#Leases"/>
  <link:definitionLink xlink:role="http://asana.com/role/Leases" xlink:type="extended" id="i0817ba3979184d6f8096dfd88520d1a1_Leases"/>
  <link:roleRef roleURI="http://asana.com/role/LeasesTables" xlink:type="simple" xlink:href="asan-20210131.xsd#LeasesTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/LeasesTables" xlink:type="extended" id="i51f34dea154a48b988e92930f65a14f9_LeasesTables"/>
  <link:roleRef roleURI="http://asana.com/role/LeasesNarrativeDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#LeasesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/LeasesNarrativeDetails" xlink:type="extended" id="i78e51cf7f1254b75b059add877590750_LeasesNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_09ef1d5f-b9ee-4511-b650-3e8055675389" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_LeaseCostNetOfRentalIncome_01d9c808-f5d0-4968-9a65-bf9b7c5cfa1e" xlink:href="asan-20210131.xsd#asan_LeaseCostNetOfRentalIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_09ef1d5f-b9ee-4511-b650-3e8055675389" xlink:to="loc_asan_LeaseCostNetOfRentalIncome_01d9c808-f5d0-4968-9a65-bf9b7c5cfa1e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LettersOfCreditOutstandingAmount_cf5e7050-a8ec-4569-b1ae-b7b8994e36b6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LettersOfCreditOutstandingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_09ef1d5f-b9ee-4511-b650-3e8055675389" xlink:to="loc_us-gaap_LettersOfCreditOutstandingAmount_cf5e7050-a8ec-4569-b1ae-b7b8994e36b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_cb384144-cc0f-4ab5-bb1f-b3b9c3ca86ac" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_09ef1d5f-b9ee-4511-b650-3e8055675389" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_cb384144-cc0f-4ab5-bb1f-b3b9c3ca86ac" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_103fa9c8-e7ca-47ba-a8b0-deedd29562a3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_09ef1d5f-b9ee-4511-b650-3e8055675389" xlink:to="loc_us-gaap_OperatingLeaseLiability_103fa9c8-e7ca-47ba-a8b0-deedd29562a3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_LesseeOperatingLeaseFutureMinimumPaymentsNetOfTenantImprovementsReceivable_fe0bbb69-6b29-4cfe-9c36-6a99387ed33d" xlink:href="asan-20210131.xsd#asan_LesseeOperatingLeaseFutureMinimumPaymentsNetOfTenantImprovementsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_09ef1d5f-b9ee-4511-b650-3e8055675389" xlink:to="loc_asan_LesseeOperatingLeaseFutureMinimumPaymentsNetOfTenantImprovementsReceivable_fe0bbb69-6b29-4cfe-9c36-6a99387ed33d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_LesseeOperatingLeaseLiabilityUndiscountedAmountNet_b38bc150-a4fa-447b-b64f-9ca0efde9c6f" xlink:href="asan-20210131.xsd#asan_LesseeOperatingLeaseLiabilityUndiscountedAmountNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_09ef1d5f-b9ee-4511-b650-3e8055675389" xlink:to="loc_asan_LesseeOperatingLeaseLiabilityUndiscountedAmountNet_b38bc150-a4fa-447b-b64f-9ca0efde9c6f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_LesseeOperatingLeaseFeesUndiscountedAmount_8a64979c-5702-412b-8d28-f5859e052150" xlink:href="asan-20210131.xsd#asan_LesseeOperatingLeaseFeesUndiscountedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_09ef1d5f-b9ee-4511-b650-3e8055675389" xlink:to="loc_asan_LesseeOperatingLeaseFeesUndiscountedAmount_8a64979c-5702-412b-8d28-f5859e052150" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_113a052d-475d-4297-a69c-7cc7f23f860c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_09ef1d5f-b9ee-4511-b650-3e8055675389" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_113a052d-475d-4297-a69c-7cc7f23f860c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_f3d5173b-0d4e-4217-9161-c2c127c0be5b" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_113a052d-475d-4297-a69c-7cc7f23f860c" xlink:to="loc_srt_StatementGeographicalAxis_f3d5173b-0d4e-4217-9161-c2c127c0be5b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_f3d5173b-0d4e-4217-9161-c2c127c0be5b_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_f3d5173b-0d4e-4217-9161-c2c127c0be5b" xlink:to="loc_srt_SegmentGeographicalDomain_f3d5173b-0d4e-4217-9161-c2c127c0be5b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_7ea9eb64-5fd9-4db4-a6ce-ab84d3bc019e" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_f3d5173b-0d4e-4217-9161-c2c127c0be5b" xlink:to="loc_srt_SegmentGeographicalDomain_7ea9eb64-5fd9-4db4-a6ce-ab84d3bc019e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SanFranciscoMember_33de4521-0e0b-4000-af79-11f14a0d5e64" xlink:href="asan-20210131.xsd#asan_SanFranciscoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_7ea9eb64-5fd9-4db4-a6ce-ab84d3bc019e" xlink:to="loc_asan_SanFranciscoMember_33de4521-0e0b-4000-af79-11f14a0d5e64" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/LeasesComponentsofLeaseCostDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#LeasesComponentsofLeaseCostDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/LeasesComponentsofLeaseCostDetails" xlink:type="extended" id="i7b0bdc05ce1e41b99101e7bf4b9f92b3_LeasesComponentsofLeaseCostDetails"/>
  <link:roleRef roleURI="http://asana.com/role/LeasesFutureMinimumLeasePaymentsDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#LeasesFutureMinimumLeasePaymentsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/LeasesFutureMinimumLeasePaymentsDetails" xlink:type="extended" id="ida90f09a96aa4a2c97c62ba682a0eecf_LeasesFutureMinimumLeasePaymentsDetails"/>
  <link:roleRef roleURI="http://asana.com/role/NetLossperShare" xlink:type="simple" xlink:href="asan-20210131.xsd#NetLossperShare"/>
  <link:definitionLink xlink:role="http://asana.com/role/NetLossperShare" xlink:type="extended" id="i6060730052b44b2e9bef8a936d45e243_NetLossperShare"/>
  <link:roleRef roleURI="http://asana.com/role/NetLossperShareTables" xlink:type="simple" xlink:href="asan-20210131.xsd#NetLossperShareTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/NetLossperShareTables" xlink:type="extended" id="i5095026d5d754def8c1561000848957d_NetLossperShareTables"/>
  <link:roleRef roleURI="http://asana.com/role/NetLossperShareDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#NetLossperShareDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/NetLossperShareDetails" xlink:type="extended" id="if08810ed198048cfa7ee29f31cfa56af_NetLossperShareDetails"/>
  <link:roleRef roleURI="http://asana.com/role/NetLossperShareAntidilutiveSecuritiesDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#NetLossperShareAntidilutiveSecuritiesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/NetLossperShareAntidilutiveSecuritiesDetails" xlink:type="extended" id="ia82c3229501a4dc3b4101e9ac3e08aa2_NetLossperShareAntidilutiveSecuritiesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasicLineItems_b1e72195-3716-4f1b-8a37-8d5d998fec7b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EarningsPerShareBasicLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_659c7812-a2d1-47a1-93a8-7e62dcae53c7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareBasicLineItems_b1e72195-3716-4f1b-8a37-8d5d998fec7b" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_659c7812-a2d1-47a1-93a8-7e62dcae53c7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEarningsPerShareBasicByCommonClassTable_31f59776-63e2-4789-9cb3-833725555ea2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfEarningsPerShareBasicByCommonClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_EarningsPerShareBasicLineItems_b1e72195-3716-4f1b-8a37-8d5d998fec7b" xlink:to="loc_us-gaap_ScheduleOfEarningsPerShareBasicByCommonClassTable_31f59776-63e2-4789-9cb3-833725555ea2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_61645060-2c56-4f20-86f8-f65a58220c08" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEarningsPerShareBasicByCommonClassTable_31f59776-63e2-4789-9cb3-833725555ea2" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_61645060-2c56-4f20-86f8-f65a58220c08" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_61645060-2c56-4f20-86f8-f65a58220c08_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_61645060-2c56-4f20-86f8-f65a58220c08" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_61645060-2c56-4f20-86f8-f65a58220c08_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a5c1a062-40b8-4c15-9fa1-292964c688e6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_61645060-2c56-4f20-86f8-f65a58220c08" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a5c1a062-40b8-4c15-9fa1-292964c688e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RedeemableConvertiblePreferredStockMember_02f0c16d-7271-4dac-a968-a7227169180d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RedeemableConvertiblePreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a5c1a062-40b8-4c15-9fa1-292964c688e6" xlink:to="loc_us-gaap_RedeemableConvertiblePreferredStockMember_02f0c16d-7271-4dac-a968-a7227169180d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_7c6e0bcd-9f1a-49dd-8e4b-4bd7aa2d2f33" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a5c1a062-40b8-4c15-9fa1-292964c688e6" xlink:to="loc_us-gaap_EmployeeStockOptionMember_7c6e0bcd-9f1a-49dd-8e4b-4bd7aa2d2f33" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_3a0cea57-e2b8-4ff3-b31e-0177d84abb7e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a5c1a062-40b8-4c15-9fa1-292964c688e6" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_3a0cea57-e2b8-4ff3-b31e-0177d84abb7e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_EarlyExercisedStockOptionsMember_e90900c0-b13c-484e-b0cd-41b69f761713" xlink:href="asan-20210131.xsd#asan_EarlyExercisedStockOptionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a5c1a062-40b8-4c15-9fa1-292964c688e6" xlink:to="loc_asan_EarlyExercisedStockOptionsMember_e90900c0-b13c-484e-b0cd-41b69f761713" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_0ef5c81e-9b74-458c-a21e-2c1efee6590d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a5c1a062-40b8-4c15-9fa1-292964c688e6" xlink:to="loc_us-gaap_EmployeeStockMember_0ef5c81e-9b74-458c-a21e-2c1efee6590d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_RedeemableConvertiblePreferredStockWarrantsMember_81bab676-e3e9-4989-85b4-9f83884bb61d" xlink:href="asan-20210131.xsd#asan_RedeemableConvertiblePreferredStockWarrantsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a5c1a062-40b8-4c15-9fa1-292964c688e6" xlink:to="loc_asan_RedeemableConvertiblePreferredStockWarrantsMember_81bab676-e3e9-4989-85b4-9f83884bb61d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_8804ab30-7b31-47f6-91e8-7d74b46f41bd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_a5c1a062-40b8-4c15-9fa1-292964c688e6" xlink:to="loc_us-gaap_CommonClassBMember_8804ab30-7b31-47f6-91e8-7d74b46f41bd" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersDeficit" xlink:type="simple" xlink:href="asan-20210131.xsd#StockholdersDeficit"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersDeficit" xlink:type="extended" id="i7b5cbd06faac41bc808fc01e536e62ff_StockholdersDeficit"/>
  <link:roleRef roleURI="http://asana.com/role/StockholdersDeficitTables" xlink:type="simple" xlink:href="asan-20210131.xsd#StockholdersDeficitTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersDeficitTables" xlink:type="extended" id="i557d431fab394810a91b5d94eac6180b_StockholdersDeficitTables"/>
  <link:roleRef roleURI="http://asana.com/role/StockholdersDeficitSummaryofRedeemableConvertiblePreferredStockDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#StockholdersDeficitSummaryofRedeemableConvertiblePreferredStockDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersDeficitSummaryofRedeemableConvertiblePreferredStockDetails" xlink:type="extended" id="i1da0cd24e96a4583a2b0b934df82a80d_StockholdersDeficitSummaryofRedeemableConvertiblePreferredStockDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_9a22df59-aa3c-45f3-bf01-9b13fad89f1d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesAuthorized_6f18f525-9021-413c-8510-cf7d35de63cf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquitySharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_9a22df59-aa3c-45f3-bf01-9b13fad89f1d" xlink:to="loc_us-gaap_TemporaryEquitySharesAuthorized_6f18f525-9021-413c-8510-cf7d35de63cf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesIssued_317a87be-777d-4c87-8101-9217b0dbe579" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquitySharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_9a22df59-aa3c-45f3-bf01-9b13fad89f1d" xlink:to="loc_us-gaap_TemporaryEquitySharesIssued_317a87be-777d-4c87-8101-9217b0dbe579" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesOutstanding_d2bb2c6e-54e6-4de7-9fd4-636e4e962ded" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquitySharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_9a22df59-aa3c-45f3-bf01-9b13fad89f1d" xlink:to="loc_us-gaap_TemporaryEquitySharesOutstanding_d2bb2c6e-54e6-4de7-9fd4-636e4e962ded" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityRedemptionPricePerShare_8655264d-d38c-4192-9e43-4ae3f2b04620" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquityRedemptionPricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_9a22df59-aa3c-45f3-bf01-9b13fad89f1d" xlink:to="loc_us-gaap_TemporaryEquityRedemptionPricePerShare_8655264d-d38c-4192-9e43-4ae3f2b04620" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_db3d94ce-c6a9-4f8d-9e45-d1ac4a62bc37" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquityCarryingAmountAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_9a22df59-aa3c-45f3-bf01-9b13fad89f1d" xlink:to="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_db3d94ce-c6a9-4f8d-9e45-d1ac4a62bc37" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityLiquidationPreference_759fc3e9-9314-4bcf-8a60-f0a2321eb464" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquityLiquidationPreference"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_9a22df59-aa3c-45f3-bf01-9b13fad89f1d" xlink:to="loc_us-gaap_TemporaryEquityLiquidationPreference_759fc3e9-9314-4bcf-8a60-f0a2321eb464" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_224d9a1f-96ce-4ae1-ad5d-03eeed3c5633" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_9a22df59-aa3c-45f3-bf01-9b13fad89f1d" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_224d9a1f-96ce-4ae1-ad5d-03eeed3c5633" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_d72b9689-26ad-460b-97d3-96cbc648feca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_224d9a1f-96ce-4ae1-ad5d-03eeed3c5633" xlink:to="loc_us-gaap_PlanNameAxis_d72b9689-26ad-460b-97d3-96cbc648feca" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_d72b9689-26ad-460b-97d3-96cbc648feca_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_d72b9689-26ad-460b-97d3-96cbc648feca" xlink:to="loc_us-gaap_PlanNameDomain_d72b9689-26ad-460b-97d3-96cbc648feca_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_d72b9689-26ad-460b-97d3-96cbc648feca" xlink:to="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesOneMember_5423191c-4c53-461f-bb2a-63df19754012" xlink:href="asan-20210131.xsd#asan_SeriesOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesOneMember_5423191c-4c53-461f-bb2a-63df19754012" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesARedeemableWarrantMember_af302c08-048e-4f45-97cf-0f561ee0af0c" xlink:href="asan-20210131.xsd#asan_SeriesARedeemableWarrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesARedeemableWarrantMember_af302c08-048e-4f45-97cf-0f561ee0af0c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesAOneMember_673ff4d0-0f08-4f6b-a923-ed3b9c6cda72" xlink:href="asan-20210131.xsd#asan_SeriesAOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesAOneMember_673ff4d0-0f08-4f6b-a923-ed3b9c6cda72" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesBRedeemableWarrantMember_24d21dfe-ab13-4ad2-99e2-f21f87f65195" xlink:href="asan-20210131.xsd#asan_SeriesBRedeemableWarrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesBRedeemableWarrantMember_24d21dfe-ab13-4ad2-99e2-f21f87f65195" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesBOneMember_db5171de-0c9c-4ca2-b29d-77255adf5091" xlink:href="asan-20210131.xsd#asan_SeriesBOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesBOneMember_db5171de-0c9c-4ca2-b29d-77255adf5091" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesCMember_e3c92966-0bf1-4f03-99cc-fa2754ddf918" xlink:href="asan-20210131.xsd#asan_SeriesCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesCMember_e3c92966-0bf1-4f03-99cc-fa2754ddf918" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesCOneMember_64abd864-c51b-4ac1-b72d-d45dd87453fe" xlink:href="asan-20210131.xsd#asan_SeriesCOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesCOneMember_64abd864-c51b-4ac1-b72d-d45dd87453fe" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesDMember_97948bce-9442-4dfb-933d-d0fe82fb3d07" xlink:href="asan-20210131.xsd#asan_SeriesDMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesDMember_97948bce-9442-4dfb-933d-d0fe82fb3d07" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesDOneMember_8ddf58c5-97fc-4992-870e-cee6b9537ea4" xlink:href="asan-20210131.xsd#asan_SeriesDOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesDOneMember_8ddf58c5-97fc-4992-870e-cee6b9537ea4" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesEMember_e2cf04d0-d1f6-4df0-9631-5156727059d5" xlink:href="asan-20210131.xsd#asan_SeriesEMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesEMember_e2cf04d0-d1f6-4df0-9631-5156727059d5" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_SeriesEOneMember_07d2315c-bad7-4d3d-b51b-589f428d3ad8" xlink:href="asan-20210131.xsd#asan_SeriesEOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_1d799b95-d594-4402-83d8-2b1eaa9e943c" xlink:to="loc_asan_SeriesEOneMember_07d2315c-bad7-4d3d-b51b-589f428d3ad8" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_de291f60-8c5d-4f64-a386-2364e7a43b7b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_224d9a1f-96ce-4ae1-ad5d-03eeed3c5633" xlink:to="loc_us-gaap_StatementClassOfStockAxis_de291f60-8c5d-4f64-a386-2364e7a43b7b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_de291f60-8c5d-4f64-a386-2364e7a43b7b_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_de291f60-8c5d-4f64-a386-2364e7a43b7b" xlink:to="loc_us-gaap_ClassOfStockDomain_de291f60-8c5d-4f64-a386-2364e7a43b7b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_997dd0b5-58b0-44e1-ab08-5df42f6a18f5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_de291f60-8c5d-4f64-a386-2364e7a43b7b" xlink:to="loc_us-gaap_ClassOfStockDomain_997dd0b5-58b0-44e1-ab08-5df42f6a18f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RedeemableConvertiblePreferredStockMember_3275cea3-60e8-4402-9f15-8bbd2f373166" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RedeemableConvertiblePreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_997dd0b5-58b0-44e1-ab08-5df42f6a18f5" xlink:to="loc_us-gaap_RedeemableConvertiblePreferredStockMember_3275cea3-60e8-4402-9f15-8bbd2f373166" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersDeficitNarrativeDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#StockholdersDeficitNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersDeficitNarrativeDetails" xlink:type="extended" id="ibc39e087025e4f8fa8c6d4c767dd9eb1_StockholdersDeficitNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_a69efcef-0611-4ba0-8a81-f6c8518d0855" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_a69efcef-0611-4ba0-8a81-f6c8518d0855" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_NumberOfVotesPerShare_269fdd2f-216f-40b9-a644-a3efc59f53c0" xlink:href="asan-20210131.xsd#asan_NumberOfVotesPerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_asan_NumberOfVotesPerShare_269fdd2f-216f-40b9-a644-a3efc59f53c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConversionOfStockSharesConverted1_e77d5fcd-99ef-4c3d-9567-9b9960b46791" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConversionOfStockSharesConverted1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_ConversionOfStockSharesConverted1_e77d5fcd-99ef-4c3d-9567-9b9960b46791" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_92e67914-659d-40e7-afa8-52bc4b3d04a0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_CommonStockSharesIssued_92e67914-659d-40e7-afa8-52bc4b3d04a0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_695fd03f-1ec3-4963-8de3-9322bb748566" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_695fd03f-1ec3-4963-8de3-9322bb748566" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_6ab7018b-2ecc-4663-8158-900d3f07c8da" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_6ab7018b-2ecc-4663-8158-900d3f07c8da" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_01c63b17-4327-4230-960f-109f297d9ca0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_01c63b17-4327-4230-960f-109f297d9ca0" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_0479ccd0-3250-416e-9270-435f590888e2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_0479ccd0-3250-416e-9270-435f590888e2" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_099012b2-ba9c-4698-8214-9d5adea19185" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_099012b2-ba9c-4698-8214-9d5adea19185" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAdjustmentOfWarrants_46df3e48-09b3-446d-a82f-17dbf9c8c5e0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAdjustmentOfWarrants"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_FairValueAdjustmentOfWarrants_46df3e48-09b3-446d-a82f-17dbf9c8c5e0" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensationForfeited_0b7e77d6-edab-4940-9834-3d2201d07e7a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensationForfeited"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensationForfeited_0b7e77d6-edab-4940-9834-3d2201d07e7a" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesIssued_1e6adb1a-95ea-48e9-ae97-594e0932dd0d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_SharesIssued_1e6adb1a-95ea-48e9-ae97-594e0932dd0d" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_7b11b274-e78b-41bb-83a5-e9fe60e870f4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_7b11b274-e78b-41bb-83a5-e9fe60e870f4" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardMinimumExercisePricePercentOfFairValueGrantDatePrice_669bec12-4bdd-484d-8be5-7653b0492d82" xlink:href="asan-20210131.xsd#asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardMinimumExercisePricePercentOfFairValueGrantDatePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardMinimumExercisePricePercentOfFairValueGrantDatePrice_669bec12-4bdd-484d-8be5-7653b0492d82" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_a1dee4b1-0e72-40e9-b4fa-00c4345d186e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_a1dee4b1-0e72-40e9-b4fa-00c4345d186e" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage_ad7d7bc8-20a8-40a0-9541-00e0ec4f030c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage_ad7d7bc8-20a8-40a0-9541-00e0ec4f030c" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeBenefitsAndShareBasedCompensation_ae8dcbf7-b423-4fcc-8d10-4e73f6b9a0ac" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeBenefitsAndShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_EmployeeBenefitsAndShareBasedCompensation_ae8dcbf7-b423-4fcc-8d10-4e73f6b9a0ac" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodShares_87f54a24-7854-4156-9877-d027f2114fee" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockRepurchasedDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodShares_87f54a24-7854-4156-9877-d027f2114fee" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_2fa624f9-768d-46c7-9ea8-3f1341a42912" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsForRepurchaseOfCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_2fa624f9-768d-46c7-9ea8-3f1341a42912" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharePrice_f3ebe93d-6f8c-446e-9ee8-1fc4f412e00e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_SharePrice_f3ebe93d-6f8c-446e-9ee8-1fc4f412e00e" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_b60cd6e5-176e-40d3-9c54-b0e7e2ca9d95" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_b60cd6e5-176e-40d3-9c54-b0e7e2ca9d95" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_f821022e-be76-442e-89e2-2c817b33c2ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_f821022e-be76-442e-89e2-2c817b33c2ed" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_6cf751f6-eee5-4645-8737-3c6e20ef0cd6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_6cf751f6-eee5-4645-8737-3c6e20ef0cd6" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_ShareBasedPaymentArrangementEmployeeContributionsWithheld_17ed008e-8a7d-4730-b062-0e3d5628da63" xlink:href="asan-20210131.xsd#asan_ShareBasedPaymentArrangementEmployeeContributionsWithheld"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_asan_ShareBasedPaymentArrangementEmployeeContributionsWithheld_17ed008e-8a7d-4730-b062-0e3d5628da63" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_98bced6d-8657-4456-bd7c-884b85c2b27c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_98bced6d-8657-4456-bd7c-884b85c2b27c" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_f10b7adc-5957-4dd7-be6b-fb29798e936d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_f10b7adc-5957-4dd7-be6b-fb29798e936d" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_7923652d-ea0d-4562-8bb4-072dadd565f2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad92aa9b-c244-4dfa-9688-36ba4853af09" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_7923652d-ea0d-4562-8bb4-072dadd565f2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_5af6efa5-bd56-4101-bb0c-73d648143b19" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_7923652d-ea0d-4562-8bb4-072dadd565f2" xlink:to="loc_us-gaap_StatementClassOfStockAxis_5af6efa5-bd56-4101-bb0c-73d648143b19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_5af6efa5-bd56-4101-bb0c-73d648143b19_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_5af6efa5-bd56-4101-bb0c-73d648143b19" xlink:to="loc_us-gaap_ClassOfStockDomain_5af6efa5-bd56-4101-bb0c-73d648143b19_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_2a7bf83f-070a-4121-8a2f-b1ccd4c3599e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_5af6efa5-bd56-4101-bb0c-73d648143b19" xlink:to="loc_us-gaap_ClassOfStockDomain_2a7bf83f-070a-4121-8a2f-b1ccd4c3599e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_6baad99f-bab7-4f36-b220-cd526d6a1150" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_2a7bf83f-070a-4121-8a2f-b1ccd4c3599e" xlink:to="loc_us-gaap_CommonClassAMember_6baad99f-bab7-4f36-b220-cd526d6a1150" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_5a49e966-b946-46e6-b606-1af40665734e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_2a7bf83f-070a-4121-8a2f-b1ccd4c3599e" xlink:to="loc_us-gaap_CommonClassBMember_5a49e966-b946-46e6-b606-1af40665734e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_RedeemableConvertiblePreferredStockWarrantsMember_74799035-9d29-4de9-86df-e1fd7bfd11ac" xlink:href="asan-20210131.xsd#asan_RedeemableConvertiblePreferredStockWarrantsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_2a7bf83f-070a-4121-8a2f-b1ccd4c3599e" xlink:to="loc_asan_RedeemableConvertiblePreferredStockWarrantsMember_74799035-9d29-4de9-86df-e1fd7bfd11ac" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_e7427715-14ed-474a-be25-72313e68ec16" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_7923652d-ea0d-4562-8bb4-072dadd565f2" xlink:to="loc_us-gaap_AwardTypeAxis_e7427715-14ed-474a-be25-72313e68ec16" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e7427715-14ed-474a-be25-72313e68ec16_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_e7427715-14ed-474a-be25-72313e68ec16" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e7427715-14ed-474a-be25-72313e68ec16_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_24d0fbe1-cde4-4dc1-a0f4-8af4c0b41430" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_e7427715-14ed-474a-be25-72313e68ec16" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_24d0fbe1-cde4-4dc1-a0f4-8af4c0b41430" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_13de6fdd-f70c-4acc-b6bc-6510131af2bc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_24d0fbe1-cde4-4dc1-a0f4-8af4c0b41430" xlink:to="loc_us-gaap_EmployeeStockOptionMember_13de6fdd-f70c-4acc-b6bc-6510131af2bc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_4b31e0de-1ac3-45e8-af46-df6bcfe0a620" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_24d0fbe1-cde4-4dc1-a0f4-8af4c0b41430" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_4b31e0de-1ac3-45e8-af46-df6bcfe0a620" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_5ad4f8ea-8c06-4844-a0d8-6d3b888be54a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_24d0fbe1-cde4-4dc1-a0f4-8af4c0b41430" xlink:to="loc_us-gaap_EmployeeStockMember_5ad4f8ea-8c06-4844-a0d8-6d3b888be54a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingAxis_c096ef8e-30b6-4c64-bdbe-f772eb63f285" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VestingAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_7923652d-ea0d-4562-8bb4-072dadd565f2" xlink:to="loc_us-gaap_VestingAxis_c096ef8e-30b6-4c64-bdbe-f772eb63f285" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingDomain_c096ef8e-30b6-4c64-bdbe-f772eb63f285_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VestingDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VestingAxis_c096ef8e-30b6-4c64-bdbe-f772eb63f285" xlink:to="loc_us-gaap_VestingDomain_c096ef8e-30b6-4c64-bdbe-f772eb63f285_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingDomain_8273487d-2cd3-422f-af8a-3f4ba96f5e03" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VestingDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VestingAxis_c096ef8e-30b6-4c64-bdbe-f772eb63f285" xlink:to="loc_us-gaap_VestingDomain_8273487d-2cd3-422f-af8a-3f4ba96f5e03" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationAwardTrancheOneMember_48ce4ab6-3874-4229-8ae4-b68379ea85b8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationAwardTrancheOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VestingDomain_8273487d-2cd3-422f-af8a-3f4ba96f5e03" xlink:to="loc_us-gaap_ShareBasedCompensationAwardTrancheOneMember_48ce4ab6-3874-4229-8ae4-b68379ea85b8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationAwardTrancheTwoMember_7ded4966-a9da-4028-95fa-91272f4dc7a8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationAwardTrancheTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VestingDomain_8273487d-2cd3-422f-af8a-3f4ba96f5e03" xlink:to="loc_us-gaap_ShareBasedCompensationAwardTrancheTwoMember_7ded4966-a9da-4028-95fa-91272f4dc7a8" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersDeficitScheduleofOptionActivityDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#StockholdersDeficitScheduleofOptionActivityDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersDeficitScheduleofOptionActivityDetails" xlink:type="extended" id="i7991321668cd44c386a4906be177453d_StockholdersDeficitScheduleofOptionActivityDetails"/>
  <link:roleRef roleURI="http://asana.com/role/StockholdersDeficitFairValueAssumptionsDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#StockholdersDeficitFairValueAssumptionsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersDeficitFairValueAssumptionsDetails" xlink:type="extended" id="i3610f931ae964fbbb90878b3c7ff055e_StockholdersDeficitFairValueAssumptionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e1b0b914-2734-42fc-bbb1-7deb2c7e5214" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_16f35c86-cf48-49a4-b5e3-13ff8be212b2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e1b0b914-2734-42fc-bbb1-7deb2c7e5214" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_16f35c86-cf48-49a4-b5e3-13ff8be212b2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_817f0998-c66a-4bc6-9bed-fcf0d1d63bf3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e1b0b914-2734-42fc-bbb1-7deb2c7e5214" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_817f0998-c66a-4bc6-9bed-fcf0d1d63bf3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_5da9b616-d3da-4273-9eab-456164d789be" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e1b0b914-2734-42fc-bbb1-7deb2c7e5214" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_5da9b616-d3da-4273-9eab-456164d789be" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_bd209db3-b361-42ee-80c2-80aad34e0027" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e1b0b914-2734-42fc-bbb1-7deb2c7e5214" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_bd209db3-b361-42ee-80c2-80aad34e0027" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019e4f8b-1140-48d8-91d0-faa2a877cf3a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e1b0b914-2734-42fc-bbb1-7deb2c7e5214" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019e4f8b-1140-48d8-91d0-faa2a877cf3a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_e935b1b4-bed2-446b-af35-0b47855fb181" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019e4f8b-1140-48d8-91d0-faa2a877cf3a" xlink:to="loc_srt_RangeAxis_e935b1b4-bed2-446b-af35-0b47855fb181" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_e935b1b4-bed2-446b-af35-0b47855fb181_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_e935b1b4-bed2-446b-af35-0b47855fb181" xlink:to="loc_srt_RangeMember_e935b1b4-bed2-446b-af35-0b47855fb181_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ddd7856d-bf66-4b09-9a6c-5e9fecf010dd" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_e935b1b4-bed2-446b-af35-0b47855fb181" xlink:to="loc_srt_RangeMember_ddd7856d-bf66-4b09-9a6c-5e9fecf010dd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_19e37ded-d6f7-454d-a4dd-9f71e16b7f4e" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ddd7856d-bf66-4b09-9a6c-5e9fecf010dd" xlink:to="loc_srt_MinimumMember_19e37ded-d6f7-454d-a4dd-9f71e16b7f4e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_c6db3a42-70fb-41d9-ab36-1b321e86ede6" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ddd7856d-bf66-4b09-9a6c-5e9fecf010dd" xlink:to="loc_srt_MaximumMember_c6db3a42-70fb-41d9-ab36-1b321e86ede6" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersDeficitScheduleofRSUActivityDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#StockholdersDeficitScheduleofRSUActivityDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersDeficitScheduleofRSUActivityDetails" xlink:type="extended" id="ib18d278033f640e7b25aa93099fefbaa_StockholdersDeficitScheduleofRSUActivityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6e7808cd-ef7b-4f61-aaf3-b6b2ff09e195" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_90718abe-1dfc-4d94-ba67-77adc5c821ea" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6e7808cd-ef7b-4f61-aaf3-b6b2ff09e195" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_90718abe-1dfc-4d94-ba67-77adc5c821ea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_414c524a-c0a7-42f3-9d89-315b41ce0981" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_90718abe-1dfc-4d94-ba67-77adc5c821ea" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_414c524a-c0a7-42f3-9d89-315b41ce0981" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_b044b550-0690-4c10-88e8-6a36b7adb480" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_90718abe-1dfc-4d94-ba67-77adc5c821ea" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_b044b550-0690-4c10-88e8-6a36b7adb480" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_aad0b4e8-8253-4e15-a1e8-48b17d4a15fc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_90718abe-1dfc-4d94-ba67-77adc5c821ea" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_aad0b4e8-8253-4e15-a1e8-48b17d4a15fc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_cf6e3c2b-4548-4975-b498-82eac7321784" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_90718abe-1dfc-4d94-ba67-77adc5c821ea" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_cf6e3c2b-4548-4975-b498-82eac7321784" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_844fba14-9978-4f8c-9232-93f122bd0112" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotReleased_14331a85-27ba-41f6-bb78-d7d97329dfbe" xlink:href="asan-20210131.xsd#asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotReleased"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6e7808cd-ef7b-4f61-aaf3-b6b2ff09e195" xlink:to="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotReleased_14331a85-27ba-41f6-bb78-d7d97329dfbe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_e530b914-03cb-4c4d-b5a0-ebe4712135eb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6e7808cd-ef7b-4f61-aaf3-b6b2ff09e195" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_e530b914-03cb-4c4d-b5a0-ebe4712135eb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_aa46caab-2125-42b9-8aa3-f6b69e4463d1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_e530b914-03cb-4c4d-b5a0-ebe4712135eb" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_aa46caab-2125-42b9-8aa3-f6b69e4463d1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_61153474-1674-4694-bc4b-328a904fd937" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_e530b914-03cb-4c4d-b5a0-ebe4712135eb" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_61153474-1674-4694-bc4b-328a904fd937" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_a0f431b9-35ea-4139-b0ed-fe75247fd05c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_e530b914-03cb-4c4d-b5a0-ebe4712135eb" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_a0f431b9-35ea-4139-b0ed-fe75247fd05c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_071fba32-ea57-4dfc-bd7f-b84b938940f2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_e530b914-03cb-4c4d-b5a0-ebe4712135eb" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_071fba32-ea57-4dfc-bd7f-b84b938940f2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_6f6fdbe0-c2bd-4911-8043-f1aad12dc8dd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotSettledWeightedAverageGrantDateFairValue_199e25d0-97be-4b71-bfb8-be6adf6f3501" xlink:href="asan-20210131.xsd#asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotSettledWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6e7808cd-ef7b-4f61-aaf3-b6b2ff09e195" xlink:to="loc_asan_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodNotSettledWeightedAverageGrantDateFairValue_199e25d0-97be-4b71-bfb8-be6adf6f3501" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardEquityInstrumentsOtherThanOptionsAggregateIntrinsicValueOutstanding_eab9c6f1-69bb-44b4-b283-92d2da05ec46" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardEquityInstrumentsOtherThanOptionsAggregateIntrinsicValueOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6e7808cd-ef7b-4f61-aaf3-b6b2ff09e195" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardEquityInstrumentsOtherThanOptionsAggregateIntrinsicValueOutstanding_eab9c6f1-69bb-44b4-b283-92d2da05ec46" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_fec252f6-b85f-4c7b-80d1-a358870d27c4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_6e7808cd-ef7b-4f61-aaf3-b6b2ff09e195" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_fec252f6-b85f-4c7b-80d1-a358870d27c4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_dd556c25-6cd6-42c4-a26d-5da2ed21a1dd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_fec252f6-b85f-4c7b-80d1-a358870d27c4" xlink:to="loc_us-gaap_AwardTypeAxis_dd556c25-6cd6-42c4-a26d-5da2ed21a1dd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_dd556c25-6cd6-42c4-a26d-5da2ed21a1dd_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_dd556c25-6cd6-42c4-a26d-5da2ed21a1dd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_dd556c25-6cd6-42c4-a26d-5da2ed21a1dd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c4e2c44d-716b-4699-a091-c55b0cad6df6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_dd556c25-6cd6-42c4-a26d-5da2ed21a1dd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c4e2c44d-716b-4699-a091-c55b0cad6df6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_524682be-ac2a-4539-9baf-170d95bf9095" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c4e2c44d-716b-4699-a091-c55b0cad6df6" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_524682be-ac2a-4539-9baf-170d95bf9095" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersDeficitStockbasedCompensationExpenseDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#StockholdersDeficitStockbasedCompensationExpenseDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersDeficitStockbasedCompensationExpenseDetails" xlink:type="extended" id="ib438ee5890ec4142ac87d0b2291df96f_StockholdersDeficitStockbasedCompensationExpenseDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad1ae0d2-a059-4a19-84b1-829df346ab88" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_ea4961e7-1225-4d58-9b9c-74d9e205fcba" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad1ae0d2-a059-4a19-84b1-829df346ab88" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_ea4961e7-1225-4d58-9b9c-74d9e205fcba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_91465bd7-8a72-4f24-a17b-67f3f5fefc5d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad1ae0d2-a059-4a19-84b1-829df346ab88" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_91465bd7-8a72-4f24-a17b-67f3f5fefc5d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_ed63bcf8-8229-417c-8171-d8e3c5078cb9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad1ae0d2-a059-4a19-84b1-829df346ab88" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_ed63bcf8-8229-417c-8171-d8e3c5078cb9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_cfd68532-7284-42d8-b9c7-c5e6ca15feb2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad1ae0d2-a059-4a19-84b1-829df346ab88" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_cfd68532-7284-42d8-b9c7-c5e6ca15feb2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms_402ff4ed-0d03-4e5f-a0af-aa3cd96fc8ce" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad1ae0d2-a059-4a19-84b1-829df346ab88" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms_402ff4ed-0d03-4e5f-a0af-aa3cd96fc8ce" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a0cd376e-4414-4353-b11c-9b792dd7617b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ad1ae0d2-a059-4a19-84b1-829df346ab88" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a0cd376e-4414-4353-b11c-9b792dd7617b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_fb6c0977-7258-4fbb-8012-6e551e27f316" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a0cd376e-4414-4353-b11c-9b792dd7617b" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_fb6c0977-7258-4fbb-8012-6e551e27f316" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_fb6c0977-7258-4fbb-8012-6e551e27f316_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_fb6c0977-7258-4fbb-8012-6e551e27f316" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_fb6c0977-7258-4fbb-8012-6e551e27f316_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_c747553a-498e-473f-ba59-5a656f057c4f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_fb6c0977-7258-4fbb-8012-6e551e27f316" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_c747553a-498e-473f-ba59-5a656f057c4f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_75421349-af9a-4596-a995-d36b97ab0e39" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_c747553a-498e-473f-ba59-5a656f057c4f" xlink:to="loc_us-gaap_CostOfSalesMember_75421349-af9a-4596-a995-d36b97ab0e39" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_4166ca61-bca5-41f9-b2a5-fa89dda5bb8a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_c747553a-498e-473f-ba59-5a656f057c4f" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_4166ca61-bca5-41f9-b2a5-fa89dda5bb8a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpenseMember_4ffd3078-4b00-4ec0-80d8-223de3d85bea" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SellingAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_c747553a-498e-473f-ba59-5a656f057c4f" xlink:to="loc_us-gaap_SellingAndMarketingExpenseMember_4ffd3078-4b00-4ec0-80d8-223de3d85bea" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_73f67d59-6221-4fba-916d-52bf3ed0f0a1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_c747553a-498e-473f-ba59-5a656f057c4f" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_73f67d59-6221-4fba-916d-52bf3ed0f0a1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_948f9283-66fc-4b98-b163-0d6f6abc7ae4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a0cd376e-4414-4353-b11c-9b792dd7617b" xlink:to="loc_us-gaap_AwardTypeAxis_948f9283-66fc-4b98-b163-0d6f6abc7ae4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_948f9283-66fc-4b98-b163-0d6f6abc7ae4_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_948f9283-66fc-4b98-b163-0d6f6abc7ae4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_948f9283-66fc-4b98-b163-0d6f6abc7ae4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e26f061e-d52d-43f3-9ab9-c1d66bd01959" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_948f9283-66fc-4b98-b163-0d6f6abc7ae4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e26f061e-d52d-43f3-9ab9-c1d66bd01959" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_cea27da6-c29b-40bd-b02b-569639d6efaf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e26f061e-d52d-43f3-9ab9-c1d66bd01959" xlink:to="loc_us-gaap_EmployeeStockOptionMember_cea27da6-c29b-40bd-b02b-569639d6efaf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_2a6a85dc-3c4b-4b0d-bde2-c9120a1d1389" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e26f061e-d52d-43f3-9ab9-c1d66bd01959" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_2a6a85dc-3c4b-4b0d-bde2-c9120a1d1389" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/StockholdersDeficitESPPFairValueAssumptionsDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#StockholdersDeficitESPPFairValueAssumptionsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/StockholdersDeficitESPPFairValueAssumptionsDetails" xlink:type="extended" id="i6224392670ed48038b2647543fc15663_StockholdersDeficitESPPFairValueAssumptionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e646a8f0-c191-4879-a73c-d4d9bce144b8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_bb949cf1-a36d-47c1-a4f3-70044f2175ba" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e646a8f0-c191-4879-a73c-d4d9bce144b8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_bb949cf1-a36d-47c1-a4f3-70044f2175ba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_21a7e112-f029-4772-a34a-7c535cfb6a51" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e646a8f0-c191-4879-a73c-d4d9bce144b8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_21a7e112-f029-4772-a34a-7c535cfb6a51" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_7d1e7e7c-8efd-46a4-be2e-b8fbb78543c3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e646a8f0-c191-4879-a73c-d4d9bce144b8" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_7d1e7e7c-8efd-46a4-be2e-b8fbb78543c3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_a200a5b8-858a-4a77-8d09-8d7566a92a0d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e646a8f0-c191-4879-a73c-d4d9bce144b8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_a200a5b8-858a-4a77-8d09-8d7566a92a0d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f09edc5b-a4a7-44ce-b310-d4d50110fef8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_e646a8f0-c191-4879-a73c-d4d9bce144b8" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f09edc5b-a4a7-44ce-b310-d4d50110fef8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_f1fe4f2e-83b7-4bf6-a558-88cb26887834" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f09edc5b-a4a7-44ce-b310-d4d50110fef8" xlink:to="loc_us-gaap_AwardTypeAxis_f1fe4f2e-83b7-4bf6-a558-88cb26887834" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f1fe4f2e-83b7-4bf6-a558-88cb26887834_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_f1fe4f2e-83b7-4bf6-a558-88cb26887834" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f1fe4f2e-83b7-4bf6-a558-88cb26887834_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8f57855e-0657-4035-934d-6271591b597b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_f1fe4f2e-83b7-4bf6-a558-88cb26887834" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8f57855e-0657-4035-934d-6271591b597b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_6fc87385-ac24-43b2-be5a-b42c8c131ac5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8f57855e-0657-4035-934d-6271591b597b" xlink:to="loc_us-gaap_EmployeeStockMember_6fc87385-ac24-43b2-be5a-b42c8c131ac5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_8ef71ff2-c02b-4c66-865f-1cf4694e8f8f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f09edc5b-a4a7-44ce-b310-d4d50110fef8" xlink:to="loc_srt_RangeAxis_8ef71ff2-c02b-4c66-865f-1cf4694e8f8f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_8ef71ff2-c02b-4c66-865f-1cf4694e8f8f_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_8ef71ff2-c02b-4c66-865f-1cf4694e8f8f" xlink:to="loc_srt_RangeMember_8ef71ff2-c02b-4c66-865f-1cf4694e8f8f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_c90bbdfd-ce0a-4e87-9da6-c10092827627" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_8ef71ff2-c02b-4c66-865f-1cf4694e8f8f" xlink:to="loc_srt_RangeMember_c90bbdfd-ce0a-4e87-9da6-c10092827627" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_a0611464-23e0-4116-9681-f6b8bc953363" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_c90bbdfd-ce0a-4e87-9da6-c10092827627" xlink:to="loc_srt_MinimumMember_a0611464-23e0-4116-9681-f6b8bc953363" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_c1937d01-6a38-4a49-bcbf-8115ed4bae8e" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_c90bbdfd-ce0a-4e87-9da6-c10092827627" xlink:to="loc_srt_MaximumMember_c1937d01-6a38-4a49-bcbf-8115ed4bae8e" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/CompensationRelatedCostsPostemploymentBenefits" xlink:type="simple" xlink:href="asan-20210131.xsd#CompensationRelatedCostsPostemploymentBenefits"/>
  <link:definitionLink xlink:role="http://asana.com/role/CompensationRelatedCostsPostemploymentBenefits" xlink:type="extended" id="if40a72ead6ba4c0599f7a253dfbd4198_CompensationRelatedCostsPostemploymentBenefits"/>
  <link:roleRef roleURI="http://asana.com/role/EmployeeBenefitPlansNarrativeDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#EmployeeBenefitPlansNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/EmployeeBenefitPlansNarrativeDetails" xlink:type="extended" id="i3c540ce12e7f4e3a88e4cc9fd08f846f_EmployeeBenefitPlansNarrativeDetails"/>
  <link:roleRef roleURI="http://asana.com/role/InterestIncomeandOtherIncomeExpenseNet" xlink:type="simple" xlink:href="asan-20210131.xsd#InterestIncomeandOtherIncomeExpenseNet"/>
  <link:definitionLink xlink:role="http://asana.com/role/InterestIncomeandOtherIncomeExpenseNet" xlink:type="extended" id="i866d2f6e273f4caeb78cb11c535abe7a_InterestIncomeandOtherIncomeExpenseNet"/>
  <link:roleRef roleURI="http://asana.com/role/InterestIncomeandOtherIncomeExpenseNetTables" xlink:type="simple" xlink:href="asan-20210131.xsd#InterestIncomeandOtherIncomeExpenseNetTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/InterestIncomeandOtherIncomeExpenseNetTables" xlink:type="extended" id="icfa07db6f860499e91a9d3f150374f65_InterestIncomeandOtherIncomeExpenseNetTables"/>
  <link:roleRef roleURI="http://asana.com/role/InterestIncomeandOtherIncomeExpenseNetDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#InterestIncomeandOtherIncomeExpenseNetDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/InterestIncomeandOtherIncomeExpenseNetDetails" xlink:type="extended" id="i37ebc0be9ef54b2baa5a7061b23d71bd_InterestIncomeandOtherIncomeExpenseNetDetails"/>
  <link:roleRef roleURI="http://asana.com/role/IncomeTaxes" xlink:type="simple" xlink:href="asan-20210131.xsd#IncomeTaxes"/>
  <link:definitionLink xlink:role="http://asana.com/role/IncomeTaxes" xlink:type="extended" id="i2fc8b057f24e4cf3b07aad49eca837dd_IncomeTaxes"/>
  <link:roleRef roleURI="http://asana.com/role/IncomeTaxesTables" xlink:type="simple" xlink:href="asan-20210131.xsd#IncomeTaxesTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/IncomeTaxesTables" xlink:type="extended" id="i8a139f68697d40bb9745291e94bf4662_IncomeTaxesTables"/>
  <link:roleRef roleURI="http://asana.com/role/IncomeTaxesComponentsofIncomeandIncomeTaxExpenseDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#IncomeTaxesComponentsofIncomeandIncomeTaxExpenseDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/IncomeTaxesComponentsofIncomeandIncomeTaxExpenseDetails" xlink:type="extended" id="i725d6cbd0e914e9f8bf7399bd93341c7_IncomeTaxesComponentsofIncomeandIncomeTaxExpenseDetails"/>
  <link:roleRef roleURI="http://asana.com/role/IncomeTaxesComponentsofIncomeLossBeforeTaxesDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#IncomeTaxesComponentsofIncomeLossBeforeTaxesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/IncomeTaxesComponentsofIncomeLossBeforeTaxesDetails" xlink:type="extended" id="i083f06a683d94516bc34f3dfc2b44e53_IncomeTaxesComponentsofIncomeLossBeforeTaxesDetails"/>
  <link:roleRef roleURI="http://asana.com/role/IncomeTaxesReconciliationofEffectiveTaxRatesDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#IncomeTaxesReconciliationofEffectiveTaxRatesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/IncomeTaxesReconciliationofEffectiveTaxRatesDetails" xlink:type="extended" id="i48f4c40fb8b94ac6b6e8151474a247f0_IncomeTaxesReconciliationofEffectiveTaxRatesDetails"/>
  <link:roleRef roleURI="http://asana.com/role/IncomeTaxesComponentsofDeferredTaxAssetsandLiabilitiesDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#IncomeTaxesComponentsofDeferredTaxAssetsandLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/IncomeTaxesComponentsofDeferredTaxAssetsandLiabilitiesDetails" xlink:type="extended" id="i7047092975284cfb8d41ae7d88da9af2_IncomeTaxesComponentsofDeferredTaxAssetsandLiabilitiesDetails"/>
  <link:roleRef roleURI="http://asana.com/role/IncomeTaxesNarrativeDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#IncomeTaxesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/IncomeTaxesNarrativeDetails" xlink:type="extended" id="ia10027599e3e49118cf09c95a682002a_IncomeTaxesNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxContingencyLineItems_b424c0c7-14c7-426d-a51c-9dfdad959d83" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxContingencyLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowanceDeferredTaxAssetChangeInAmount_c22de523-ab44-4954-b494-438a1596c357" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationAllowanceDeferredTaxAssetChangeInAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxContingencyLineItems_b424c0c7-14c7-426d-a51c-9dfdad959d83" xlink:to="loc_us-gaap_ValuationAllowanceDeferredTaxAssetChangeInAmount_c22de523-ab44-4954-b494-438a1596c357" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwards_3ce86bb6-d15e-44af-ab24-d3e2bdc7ef56" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLossCarryforwards"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxContingencyLineItems_b424c0c7-14c7-426d-a51c-9dfdad959d83" xlink:to="loc_us-gaap_OperatingLossCarryforwards_3ce86bb6-d15e-44af-ab24-d3e2bdc7ef56" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch_63d018a0-3627-4a7f-bd94-c2ccc77bcc27" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxContingencyLineItems_b424c0c7-14c7-426d-a51c-9dfdad959d83" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch_63d018a0-3627-4a7f-bd94-c2ccc77bcc27" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxContingencyTable_6e368e52-f41f-420e-a324-54ce77ce3ac9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxContingencyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_IncomeTaxContingencyLineItems_b424c0c7-14c7-426d-a51c-9dfdad959d83" xlink:to="loc_us-gaap_IncomeTaxContingencyTable_6e368e52-f41f-420e-a324-54ce77ce3ac9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityAxis_03c87f72-a611-427c-bee8-62fb3aa53011" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxAuthorityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_IncomeTaxContingencyTable_6e368e52-f41f-420e-a324-54ce77ce3ac9" xlink:to="loc_us-gaap_IncomeTaxAuthorityAxis_03c87f72-a611-427c-bee8-62fb3aa53011" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_03c87f72-a611-427c-bee8-62fb3aa53011_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_03c87f72-a611-427c-bee8-62fb3aa53011" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_03c87f72-a611-427c-bee8-62fb3aa53011_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_b05edf37-231e-41d9-80b8-505830d4fe7a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_03c87f72-a611-427c-bee8-62fb3aa53011" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_b05edf37-231e-41d9-80b8-505830d4fe7a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticCountryMember_58cc174b-e0f9-4886-8d82-8ab5b9b9bab9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DomesticCountryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_b05edf37-231e-41d9-80b8-505830d4fe7a" xlink:to="loc_us-gaap_DomesticCountryMember_58cc174b-e0f9-4886-8d82-8ab5b9b9bab9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StateAndLocalJurisdictionMember_c813f3da-00fe-4a8d-bff6-4509d94efac3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StateAndLocalJurisdictionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_b05edf37-231e-41d9-80b8-505830d4fe7a" xlink:to="loc_us-gaap_StateAndLocalJurisdictionMember_c813f3da-00fe-4a8d-bff6-4509d94efac3" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/IncomeTaxesReconciliationofUnrecognizedTaxBenefitsDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#IncomeTaxesReconciliationofUnrecognizedTaxBenefitsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/IncomeTaxesReconciliationofUnrecognizedTaxBenefitsDetails" xlink:type="extended" id="i541e00e2a3d540fe89148f6433be6c66_IncomeTaxesReconciliationofUnrecognizedTaxBenefitsDetails"/>
  <link:roleRef roleURI="http://asana.com/role/GeographicInformation" xlink:type="simple" xlink:href="asan-20210131.xsd#GeographicInformation"/>
  <link:definitionLink xlink:role="http://asana.com/role/GeographicInformation" xlink:type="extended" id="i615632ad00cc4aeab9d161e724865487_GeographicInformation"/>
  <link:roleRef roleURI="http://asana.com/role/GeographicInformationTables" xlink:type="simple" xlink:href="asan-20210131.xsd#GeographicInformationTables"/>
  <link:definitionLink xlink:role="http://asana.com/role/GeographicInformationTables" xlink:type="extended" id="i3f22e37328ca4db48f2ba9c9d08e48e3_GeographicInformationTables"/>
  <link:roleRef roleURI="http://asana.com/role/GeographicInformationDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#GeographicInformationDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/GeographicInformationDetails" xlink:type="extended" id="i2ba514b6667046988b836219bf5813fd_GeographicInformationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_92374576-9beb-40cd-9fe7-fd4631ed7268" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentReportingRevenueReconcilingItemLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_614cee1d-e738-4e5d-b503-307a407b3302" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_92374576-9beb-40cd-9fe7-fd4631ed7268" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_614cee1d-e738-4e5d-b503-307a407b3302" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoncurrentAssets_6775683a-6ef7-415f-906e-53818e50367d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NoncurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_92374576-9beb-40cd-9fe7-fd4631ed7268" xlink:to="loc_us-gaap_NoncurrentAssets_6775683a-6ef7-415f-906e-53818e50367d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_7b451d07-3bf8-4935-9522-8be0d81e1ef6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_92374576-9beb-40cd-9fe7-fd4631ed7268" xlink:to="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_7b451d07-3bf8-4935-9522-8be0d81e1ef6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_0aea9ccb-4e35-4bdd-a152-b442f984a1c7" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_7b451d07-3bf8-4935-9522-8be0d81e1ef6" xlink:to="loc_srt_StatementGeographicalAxis_0aea9ccb-4e35-4bdd-a152-b442f984a1c7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_0aea9ccb-4e35-4bdd-a152-b442f984a1c7_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_0aea9ccb-4e35-4bdd-a152-b442f984a1c7" xlink:to="loc_srt_SegmentGeographicalDomain_0aea9ccb-4e35-4bdd-a152-b442f984a1c7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_8166dbae-6a5f-4812-8d84-06ea5d962be8" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_0aea9ccb-4e35-4bdd-a152-b442f984a1c7" xlink:to="loc_srt_SegmentGeographicalDomain_8166dbae-6a5f-4812-8d84-06ea5d962be8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_5af65113-afa8-4683-acef-31143d23c03c" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_8166dbae-6a5f-4812-8d84-06ea5d962be8" xlink:to="loc_country_US_5af65113-afa8-4683-acef-31143d23c03c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_678cbe32-0612-493e-aabf-5a96e785b2fa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_8166dbae-6a5f-4812-8d84-06ea5d962be8" xlink:to="loc_us-gaap_NonUsMember_678cbe32-0612-493e-aabf-5a96e785b2fa" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://asana.com/role/RelatedPartyTransactions" xlink:type="simple" xlink:href="asan-20210131.xsd#RelatedPartyTransactions"/>
  <link:definitionLink xlink:role="http://asana.com/role/RelatedPartyTransactions" xlink:type="extended" id="i97827299108e47158e328fc2649564ad_RelatedPartyTransactions"/>
  <link:roleRef roleURI="http://asana.com/role/RelatedPartyTransactionsDetails" xlink:type="simple" xlink:href="asan-20210131.xsd#RelatedPartyTransactionsDetails"/>
  <link:definitionLink xlink:role="http://asana.com/role/RelatedPartyTransactionsDetails" xlink:type="extended" id="i746a182893ff46b981252cc7138bb816_RelatedPartyTransactionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_5a13aa44-6f3c-456d-acf8-b7a8e18c7c40" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_NotesReceivableRelatedPartiesAmountAuthorized_c269adba-0286-4a5b-8ebd-4104fc2554d1" xlink:href="asan-20210131.xsd#asan_NotesReceivableRelatedPartiesAmountAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_5a13aa44-6f3c-456d-acf8-b7a8e18c7c40" xlink:to="loc_asan_NotesReceivableRelatedPartiesAmountAuthorized_c269adba-0286-4a5b-8ebd-4104fc2554d1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_1feae333-d6ec-4c44-ae07-a0fe3200b76d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionAmountsOfTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_5a13aa44-6f3c-456d-acf8-b7a8e18c7c40" xlink:to="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_1feae333-d6ec-4c44-ae07-a0fe3200b76d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiability_50607745-8622-4564-993b-455de3575cdf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_5a13aa44-6f3c-456d-acf8-b7a8e18c7c40" xlink:to="loc_us-gaap_ContractWithCustomerLiability_50607745-8622-4564-993b-455de3575cdf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityRevenueRecognized_71898cbc-0418-4e49-b8a0-ecd931218b59" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityRevenueRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_5a13aa44-6f3c-456d-acf8-b7a8e18c7c40" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityRevenueRecognized_71898cbc-0418-4e49-b8a0-ecd931218b59" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableRelatedParties_a0af4bc5-1098-4e46-abc6-eb82c5f1a7ca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsReceivableRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_5a13aa44-6f3c-456d-acf8-b7a8e18c7c40" xlink:to="loc_us-gaap_AccountsReceivableRelatedParties_a0af4bc5-1098-4e46-abc6-eb82c5f1a7ca" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromRelatedParties_46ce2f50-fe0f-42d1-baff-6491825631df" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_5a13aa44-6f3c-456d-acf8-b7a8e18c7c40" xlink:to="loc_us-gaap_RevenueFromRelatedParties_46ce2f50-fe0f-42d1-baff-6491825631df" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_702dbe52-384f-46ec-9b74-94dda70e689c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_5a13aa44-6f3c-456d-acf8-b7a8e18c7c40" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_702dbe52-384f-46ec-9b74-94dda70e689c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_3dfd386c-b77e-4cb0-b57f-2668d03badd3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_702dbe52-384f-46ec-9b74-94dda70e689c" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_3dfd386c-b77e-4cb0-b57f-2668d03badd3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_3dfd386c-b77e-4cb0-b57f-2668d03badd3_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_3dfd386c-b77e-4cb0-b57f-2668d03badd3" xlink:to="loc_us-gaap_RelatedPartyDomain_3dfd386c-b77e-4cb0-b57f-2668d03badd3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_bc4af12e-f6a6-4f5f-90eb-ca7145421583" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_3dfd386c-b77e-4cb0-b57f-2668d03badd3" xlink:to="loc_us-gaap_RelatedPartyDomain_bc4af12e-f6a6-4f5f-90eb-ca7145421583" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_AffiliatedEntityMember_da67cb99-c788-4dfd-b632-fbd58551d3be" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_AffiliatedEntityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_bc4af12e-f6a6-4f5f-90eb-ca7145421583" xlink:to="loc_srt_AffiliatedEntityMember_da67cb99-c788-4dfd-b632-fbd58551d3be" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_e4ac5305-e1af-4e37-a2cf-30b3d8379ba3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_702dbe52-384f-46ec-9b74-94dda70e689c" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_e4ac5305-e1af-4e37-a2cf-30b3d8379ba3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_e4ac5305-e1af-4e37-a2cf-30b3d8379ba3_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_e4ac5305-e1af-4e37-a2cf-30b3d8379ba3" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_e4ac5305-e1af-4e37-a2cf-30b3d8379ba3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_d4d26f8d-c6c9-42f8-b736-8a8672ebf088" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_e4ac5305-e1af-4e37-a2cf-30b3d8379ba3" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_d4d26f8d-c6c9-42f8-b736-8a8672ebf088" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_LeaseExpenseMember_29eba1c2-e4c2-425f-8fb0-dfd8814dbb2d" xlink:href="asan-20210131.xsd#asan_LeaseExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_d4d26f8d-c6c9-42f8-b736-8a8672ebf088" xlink:to="loc_asan_LeaseExpenseMember_29eba1c2-e4c2-425f-8fb0-dfd8814dbb2d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_AdvertisingExpenseMember_0f2a4833-b457-4cfb-8c7a-998511991415" xlink:href="asan-20210131.xsd#asan_AdvertisingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_d4d26f8d-c6c9-42f8-b736-8a8672ebf088" xlink:to="loc_asan_AdvertisingExpenseMember_0f2a4833-b457-4cfb-8c7a-998511991415" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_asan_VariousRecurringSubscriptionAgreementsMember_b209c39f-b8de-4c43-8ff5-d94f1bd47f7e" xlink:href="asan-20210131.xsd#asan_VariousRecurringSubscriptionAgreementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_d4d26f8d-c6c9-42f8-b736-8a8672ebf088" xlink:to="loc_asan_VariousRecurringSubscriptionAgreementsMember_b209c39f-b8de-4c43-8ff5-d94f1bd47f7e" xlink:type="arc" order="2"/>
  </link:definitionLink>
</link:linkbase>
